scholarly journals Verticillium longisporum Elicits Media-Dependent Secretome Responses With Capacity to Distinguish Between Plant-Related Environments

2020 ◽  
Vol 11 ◽  
Author(s):  
Miriam Leonard ◽  
Anika Kühn ◽  
Rebekka Harting ◽  
Isabel Maurus ◽  
Alexandra Nagel ◽  
...  
Keyword(s):  
Verticillium Longisporum

scholarly journals Salicylic acid and salicylic acid glucoside in xylem sap of Brassica napus infected with Verticillium longisporum

2009 ◽  
Vol 122 (5) ◽  
pp. 571-579 ◽  
Author(s):  
Astrid Ratzinger ◽  
Nadine Riediger ◽  
Andreas von Tiedemann ◽  
Petr Karlovsky
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Xylem Sap ◽  
Verticillium Longisporum ◽  
Salicylic Acid Glucoside

scholarly journals In-depth evaluation of root infection systems using the vascular fungus Verticillium longisporum as soil-borne model pathogen

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Christian Fröschel
Keyword(s):  
Marker Genes ◽  
Root Infection ◽  
Root Pathogen ◽  
Verticillium Longisporum ◽  
Indole Glucosinolates ◽  
Advantages And Disadvantages ◽  
Root Pathogens ◽  
Pathogenicity Tests ◽  
Microbe Interactions ◽  
Defence Strategies

Abstract Background While leaves are far more accessible for analysing plant defences, roots are hidden in the soil, leading to difficulties in studying soil-borne interactions. Inoculation strategies for infecting model plants with model root pathogens are described in the literature, but it remains demanding to obtain a methodological overview. To address this challenge, this study uses the model root pathogen Verticillium longisporum on Arabidopsis thaliana host plants and provides recommendations for selecting appropriate infection systems to investigate how plants cope with root pathogens. Results A novel root infection system is introduced, while two existing ones are precisely described and optimized. Step-by-step protocols are presented and accompanied by pathogenicity tests, transcriptional analyses of indole-glucosinolate marker genes and independent confirmations using reporter constructs. Advantages and disadvantages of each infection system are assessed. Overall, the results validate the importance of indole-glucosinolates as secondary metabolites that limit the Verticillium propagation in its host plant. Conclusion Detailed assistances on studying host defence strategies and responses against V. longisporum is provided. Furthermore, other soil-borne microorganisms (e.g., V. dahliae) or model plants, such as economically important oilseed rape and tomato, can be introduced in the infection systems described. Hence, these proven manuals can support finding a root infection system for your specific research questions to further decipher root-microbe interactions.

Verticillium longisporum. [Distribution map].

2011 ◽  
Author(s):  
Keyword(s):  
Czech Republic ◽  
Brassica Napus ◽  
North America ◽  
Geographical Distribution ◽  
Distribution Map ◽  
England And Wales ◽  
Verticillium Longisporum ◽  
Southern Russia ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Verticillium longisporum (C. Starck) Karapapa, Bainbr. & Heale. Ascomycota. Hosts: rape (Brassica napus var. napus). Information is given on the geographical distribution in Europe (Belgium, Czech Republic, France, Germany, Netherlands, Poland, Russia (Southern Russia), Sweden, UK (England and Wales)), Asia (Japan), North America (USA (California, Illinois)).

scholarly journals Gene presence-absence variation associates with quantitative Verticillium longisporum disease resistance in Brassica napus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Iulian Gabur ◽  
Harmeet Singh Chawla ◽  
Daniel Teshome Lopisso ◽  
Andreas von Tiedemann ◽  
Rod J. Snowdon ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Brassica Napus ◽  
Verticillium Longisporum

scholarly journals Endophytic bacterial communities of oilseed rape associate with genotype-specific resistance against Verticillium longisporum

2019 ◽  
Vol 96 (1) ◽  
Author(s):  
Stefanie P Glaeser ◽  
Iulian Gabur ◽  
Hossein Haghighi ◽  
Jens-Ole Bartz ◽  
Peter Kämpfer ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Relative Abundance ◽  
Bacterial Communities ◽  
Quantitative Resistance ◽  
Bacterial Community Composition ◽  
Phylogenetic Group ◽  
Rrna Gene ◽  
Host Genotype ◽  
Brassica Napus L ◽  
Verticillium Longisporum

ABSTRACT Associations of endophytic bacterial community composition of oilseed rape (Brassica napus L.) with quantitative resistance against the soil-borne fungal pathogen Verticillium longisporum was assessed by 16S rRNA gene amplicon sequencing in roots and hypocotyls of four plant lines with contrasting genetic composition in regard to quantitative resistance reactions. The plant compartment was found to be the dominating driving factor for the specificity of bacterial communities in healthy plants. Furthermore, V. longisporum infection triggered a stabilization of phylogenetic group abundance in replicated samples suggesting a host genotype-specific selection. Genotype-specific associations with bacterial phylogenetic group abundance were identified by comparison of plant genotype groups (resistant versus susceptible) and treatment groups (healthy versus V. longisporum-infected) allowing dissection into constitutive and induced directional association patterns. Relative abundance of Flavobacteria, Pseudomonas, Rhizobium and Cellvibrio was associated with resistance/susceptibility. Relative abundance of Flavobacteria and Cellvibrio was increased in resistant genotypes according to their known ecological functions. In contrast, a higher relative abundance of Pseudomonas and Rhizobium, which are known to harbor many species with antagonistic properties to fungal pathogens, was found to be associated with susceptibility, indicating that these groups do not play a major role in genetically controlled resistance of oilseed rape against V. longisporum.

scholarly journals The Vascular Pathogen Verticillium longisporum Does Not Affect Water Relations and Plant Responses to Drought Stress of Its Host, Brassica napus

2017 ◽  
Vol 107 (4) ◽  
pp. 444-454 ◽  
Author(s):  
Daniel Teshome Lopisso ◽  
Jessica Knüfer ◽  
Birger Koopmann ◽  
Andreas von Tiedemann
Keyword(s):  
Drought Stress ◽  
Brassica Napus ◽  
Plant Growth ◽  
Water Relations ◽  
Growth Parameters ◽  
Molecular Genetic ◽  
Leaf Water Content ◽  
Plant Responses ◽  
Brassica Napus L ◽  
Verticillium Longisporum

Verticillium longisporum is a host-specific vascular pathogen of oilseed rape (Brassica napus L.) that causes economic crop losses by impairing plant growth and inducing premature senescence. This study investigates whether plant damage through Verticillium stem striping is due to impaired plant water relations, whether V. longisporum affects responses of a susceptible B. napus variety to drought stress, and whether drought stress, in turn, affects plant responses to V. longisporum. Two-factorial experiments on a susceptible cultivar of B. napus infected or noninfected with V. longisporum and exposed to three watering levels (30, 60, and 100% field capacity) revealed that drought stress and V. longisporum impaired plant growth by entirely different mechanisms. Although both stresses similarly affected plant growth parameters (plant height, hypocotyl diameter, and shoot and root dry matter), infection of B. napus with V. longisporum did not affect any drought-related physiological or molecular genetic plant parameters, including transpiration rate, stomatal conductance, photosynthesis rate, water use efficiency, relative leaf water content, leaf proline content, or the expression of drought-responsive genes. Thus, this study provides comprehensive physiological and molecular genetic evidence explaining the lack of wilt symptoms in B. napus infected with V. longisporum. Likewise, drought tolerance of B. napus was unaffected by V. longisporum, as was the level of disease by drought conditions, thus excluding a concerted action of both stresses in the field. Although it is evident that drought and vascular infection with V. longisporum impair plant growth by different mechanisms, it remains to be determined by which other factors V. longisporum causes crop loss.

scholarly journals Internal Resistance in Winter Oilseed Rape Inhibits Systemic Spread of the Vascular Pathogen Verticillium longisporum

2009 ◽  
Vol 99 (7) ◽  
pp. 802-811 ◽  
Author(s):  
C. Eynck ◽  
B. Koopmann ◽  
P. Karlovsky ◽  
A. von Tiedemann
Keyword(s):  
Cell Wall ◽  
Oilseed Rape ◽  
Quantitative Polymerase Chain Reaction ◽  
Defense Responses ◽  
Root Penetration ◽  
Dependent Manner ◽  
Resistant Genotype ◽  
Verticillium Longisporum ◽  
Systemic Spread ◽  
Bound Phenolics

Verticillium longisporum is a vascular fungal pathogen presently threatening oilseed rape production in Europe. Systemic spread and vascular responses were studied in a susceptible (‘Falcon’) and a resistant genotype (SEM 05-500256) of Brassica napus. Colonization of both genotypes after dip-inoculation of the roots followed by quantitative polymerase chain reaction revealed similarities only in the initial stages of root penetration and colonization of the hypocotyl, while a substantial invasion of the shoot was only recorded in ‘Falcon’. It is concluded that the type of resistance represented in SEM 05-500256 does not prevent the plant base from being invaded as it is internally expressed well after root penetration and colonization of the plant base. The morphological and biochemical nature of barriers induced in the hypocotyl tissue upon infection was studied with histochemical methods accompanied by biochemical analyses. Histochemical studies revealed the build-up of vascular occlusions and the reinforcement of tracheary elements through the deposition of cell wall-bound phenolics and lignin. Furthermore, the accumulation of soluble phenolics was observed. Although these responses were found in vascular tissues of both genotypes, they occurred with a significantly higher intensity in the resistant genotype and corresponded with the disease phenotype. In the resistant genotype phenols were differentially expressed in a time-dependent manner with preformed soluble and cell wall-bound phenolics at earlier time points and de novo formation of lignin and lignin-like polymers at later stages of infection. This is the first study identifying a crucial role of phenol metabolism in internal defense of B. napus against V. longisporum and locating the crucial defense responses in the plant hypocotyl.

Quantitative nested real-time PCR detection of Verticillium longisporum and V. dahliae in the soil of cabbage fields

2011 ◽  
Vol 77 (5) ◽  
pp. 282-291 ◽  
Author(s):  
Shinpei Banno ◽  
Hidenari Saito ◽  
Hiroshi Sakai ◽  
Toshihiko Urushibara ◽  
Kentaro Ikeda ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Verticillium Longisporum

scholarly journals The Cpc1 Regulator of the Cross-Pathway Control of Amino Acid Biosynthesis Is Required for Pathogenicity of the Vascular Pathogen Verticillium longisporum

2013 ◽  
Vol 26 (11) ◽  
pp. 1312-1324 ◽  
Author(s):  
Christian Timpner ◽  
Susanna A. Braus-Stromeyer ◽  
Van Tuan Tran ◽  
Gerhard H. Braus
Keyword(s):  
Amino Acid ◽  
Oilseed Rape ◽  
Host Plants ◽  
Pathogenic Fungus ◽  
Amino Acid Biosynthesis ◽  
Amino Acid Starvation ◽  
Acid Biosynthesis ◽  
Plant Host ◽  
Verticillium Longisporum ◽  
The Cross

The plant-pathogenic fungus Verticillium longisporum is a causal agent of early senescence and ripening in cruciferous crops like Brassica napus. Verticillium wilts have become serious agricultural threats in recent decades. Verticillium species infect host plants through the roots and colonize xylem vessels of the host plant. The xylem fluid provides an environment with limited carbon sources and unbalanced amino acid supply, which requires V. longisporum to induce the cross-pathway control of amino acid biosynthesis. RNA-mediated gene silencing reduced the expression of the two CPC1 isogenes (VlCPC1-1 and VlCPC1-2) of the allodiploid V. longisporum up to 85%. VlCPC1 encodes the conserved transcription factor of the cross-pathway control. The silenced mutants were highly sensitive to amino-acid starvation, and the infected plants showed significantly fewer symptoms such as stunting or early senescence in oilseed rape plant infection assays. Consistently, deletion of single CPC1 of the haploid V. dahliae resulted in strains that are sensitive to amino-acid starvation and cause strongly reduced symptoms in the plant-host tomato (Solanum lycopersicum). The allodiploid V. longisporum and the haploid V. dahliae are the first phytopathogenic fungi that were shown to require CPC1 for infection and colonization of their respective host plants, oilseed rape and tomato.

Characterization and distribution of two subtypes of Verticillium longisporum isolated from cabbage fields in Japan

2014 ◽  
Vol 81 (2) ◽  
pp. 118-126 ◽  
Author(s):  
Shinpei Banno ◽  
Kentaro Ikeda ◽  
Hidenari Saito ◽  
Hiroshi Sakai ◽  
Toshihiko Urushibara ◽  
...  
Keyword(s):  
Verticillium Longisporum
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