scholarly journals Metabolic Engineering of Oleaginous Yeast Yarrowia lipolytica for Overproduction of Fatty Acids

2020 ◽  
Vol 11 ◽  
Author(s):  
Rishikesh Ghogare ◽  
Shulin Chen ◽  
Xiaochao Xiong
Keyword(s):  
Fatty Acids ◽  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Yeast Yarrowia Lipolytica

Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica

2013 ◽  
Vol 98 (1) ◽  
pp. 251-262 ◽  
Author(s):  
A. Beopoulos ◽  
J. Verbeke ◽  
F. Bordes ◽  
M. Guicherd ◽  
M. Bressy ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Ricinoleic Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Oleaginous Yeast ◽  
Yeast Yarrowia Lipolytica

Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica

2012 ◽  
Vol 114 ◽  
pp. 443-449 ◽  
Author(s):  
Pierre Fontanille ◽  
Vinod Kumar ◽  
Gwendoline Christophe ◽  
Régis Nouaille ◽  
Christian Larroche
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Volatile Fatty Acids ◽  
Oleaginous Yeast ◽  
Yeast Yarrowia Lipolytica

scholarly journals Bioproducts generation from carboxylate platforms by the non-conventional yeast Yarrowia lipolytica

2021 ◽  
Author(s):  
Young-Kyoung Park ◽  
Cristina González-Fernández ◽  
Raúl Robles-Iglesias ◽  
Lea Vidal ◽  
Pierre Fontanille ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Volatile Fatty Acids ◽  
Oleaginous Yeast ◽  
Current Knowledge ◽  
Value Added ◽  
Building Blocks ◽  
Renewable Sources ◽  
The Relationship ◽  
Yeast Yarrowia Lipolytica

Abstract In recent years, there has been a growing interest in the use of renewable sources for bio-based production aiming at developing sustainable and feasible approaches towards a circular economy. Among these renewable sources, organic wastes (OWs) can be anaerobically digested to generate carboxylates like volatile fatty acids (VFAs), lactic acid, and longer-chain fatty acids that are regarded as novel building blocks for the synthesis of value-added compounds by yeasts. This review discusses on the processes that can be used to create valuable molecules from OW-derived VFAs; the pathways employed by the oleaginous yeast Yarrowia lipolytica to directly metabolize such molecules; and the relationship between OW composition, anaerobic digestion, and VFA profiles. The review also summarizes the current knowledge about VFA toxicity, the pathways by which VFAs are metabolized, and the metabolic engineering strategies that can be employed in Y. lipolytica to produce value-added biobased compounds from VFAs.

scholarly journals A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica

2017 ◽  
Vol 101 (11) ◽  
pp. 4605-4616 ◽  
Author(s):  
Nabila Imatoukene ◽  
Jonathan Verbeke ◽  
Athanasios Beopoulos ◽  
Abdelghani Idrissi Taghki ◽  
Brigitte Thomasset ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Conjugated Linoleic Acids ◽  
Metabolic Engineering Strategy ◽  
Engineering Strategy ◽  
Yeast Yarrowia Lipolytica

scholarly journals Metabolic Engineering for Unusual Lipid Production in Yarrowia lipolytica

2020 ◽  
Vol 8 (12) ◽  
pp. 1937
Author(s):  
Young-Kyoung Park ◽  
Jean-Marc Nicaud
Keyword(s):  
Fatty Acids ◽  
Metabolic Engineering ◽  
Genetic Engineering ◽  
Functional Groups ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Lipid Synthesis ◽  
Alternative Method ◽  
Endogenous Lipid ◽  
Yeast Yarrowia Lipolytica

Using microorganisms as lipid-production factories holds promise as an alternative method for generating petroleum-based chemicals. The non-conventional yeast Yarrowia lipolytica is an excellent microbial chassis; for example, it can accumulate high levels of lipids and use a broad range of substrates. Furthermore, it is a species for which an array of efficient genetic engineering tools is available. To date, extensive work has been done to metabolically engineer Y. lipolytica to produce usual and unusual lipids. Unusual lipids are scarce in nature but have several useful applications. As a result, they are increasingly becoming the targets of metabolic engineering. Unusual lipids have distinct structures; they can be generated by engineering endogenous lipid synthesis or by introducing heterologous enzymes to alter the functional groups of fatty acids. In this review, we describe current metabolic engineering strategies for improving lipid production and highlight recent researches on unusual lipid production in Y. lipolytica.

scholarly journals Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Xuan Cao ◽  
Yu-Bei Lv ◽  
Jun Chen ◽  
Tadayuki Imanaka ◽  
Liu-Jing Wei ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Yeast Yarrowia Lipolytica

scholarly journals Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sivamoke Dissook ◽  
Tomohisa Kuzuyama ◽  
Yuri Nishimoto ◽  
Shigeru Kitani ◽  
Sastia Putri ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Authentic Standard ◽  
Chemical Inhibition ◽  
Chromatographic Peaks ◽  
Methyl Erythritol Phosphate ◽  
Limiting Condition ◽  
Yeast Yarrowia Lipolytica

AbstractMethyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.

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