scholarly journals Bactericidal Effect of Entomopathogenic Bacterium Pseudomonas entomophila Against Xanthomonas citri Reduces Citrus Canker Disease Severity

2020 ◽  
Vol 11 ◽  
Author(s):  
Sonia Villamizar ◽  
Jesus Aparecido Ferro ◽  
Juan Carlos Caicedo ◽  
L. M. C. Alves
Keyword(s):  
Disease Severity ◽  
Citrus Canker ◽  
Bactericidal Effect ◽  
Xanthomonas Citri ◽  
Canker Disease ◽  
Citrus Canker Disease

scholarly journals Modified Monosaccharides Content of Xanthan Gum Impairs Citrus Canker Disease by Affecting the Epiphytic Lifestyle of Xanthomonas citri subsp. citri

2021 ◽  
Vol 9 (6) ◽  
pp. 1176
Author(s):  
Simone Cristina Picchi ◽  
Laís Moreira Granato ◽  
Maria Júlia Festa Franzini ◽  
Maxuel Oliveira Andrade ◽  
Marco Aurélio Takita ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Xanthan Gum ◽  
Wild Type Strain ◽  
Natural Infection ◽  
Citrus Canker ◽  
Wild Type ◽  
Xanthomonas Citri ◽  
Canker Disease ◽  
Key Enzyme ◽  
Citrus Canker Disease

Xanthomonas citri subsp. citri (X. citri) is a plant pathogenic bacterium causing citrus canker disease. The xanA gene encodes a phosphoglucomutase/phosphomannomutase protein that is a key enzyme required for the synthesis of lipopolysaccharides and exopolysaccharides in Xanthomonads. In this work, firstly we isolated a xanA transposon mutant (xanA::Tn5) and analyzed its phenotypes as biofilm formation, xanthan gum production, and pathogenesis on the sweet orange host. Moreover, to confirm the xanA role in the impaired phenotypes we further produced a non-polar deletion mutant (ΔxanA) and performed the complementation of both xanA mutants. In addition, we analyzed the percentages of the xanthan gum monosaccharides produced by X. citri wild-type and xanA mutant. The mutant strain had higher ratios of mannose, galactose, and xylose and lower ratios of rhamnose, glucuronic acid, and glucose than the wild-type strain. Such changes in the saccharide composition led to the reduction of xanthan yield in the xanA deficient strain, affecting also other important features in X. citri, such as biofilm formation and sliding motility. Moreover, we showed that xanA::Tn5 caused no symptoms on host leaves after spraying, a method that mimetics the natural infection condition. These results suggest that xanA plays an important role in the epiphytical stage on the leaves that is essential for the successful interaction with the host, including adaptive advantage for bacterial X. citri survival and host invasion, which culminates in pathogenicity.

scholarly journals Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis

2019 ◽  
Vol 35 (5) ◽  
pp. 486-497 ◽  
Author(s):  
Md. Nurul Islam ◽  
Md. Sarafat Ali ◽  
Seong-Jin Choi ◽  
Jae-Wook Hyun ◽  
Kwang-Hyun Baek
Keyword(s):  
Bacillus Thuringiensis ◽  
Citrus Canker ◽  
Xanthomonas Citri ◽  
Canker Disease ◽  
Citrus Canker Disease

scholarly journals The dual nature of trehalose in citrus canker disease: a virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses

2015 ◽  
Vol 66 (9) ◽  
pp. 2795-2811 ◽  
Author(s):  
Ainelén Piazza ◽  
Tamara Zimaro ◽  
Betiana S. Garavaglia ◽  
Florencia A. Ficarra ◽  
Ludivine Thomas ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Plant Defence ◽  
Citrus Canker ◽  
Xanthomonas Citri ◽  
Dual Nature ◽  
Canker Disease ◽  
Defence Responses ◽  
Plant Defence Responses ◽  
Citrus Canker Disease

scholarly journals Dynamic integration and excision of filamentous phage XacF1 in Xanthomonas citri pv. citri , the causative agent of citrus canker disease

FEBS Open Bio ◽  
2017 ◽  
Vol 7 (11) ◽  
pp. 1715-1721 ◽  
Author(s):  
Abdelmonim A. Ahmad ◽  
Makoto Kawabe ◽  
Ahmed Askora ◽  
Takeru Kawasaki ◽  
Makoto Fujie ◽  
...  
Keyword(s):  
Causative Agent ◽  
Citrus Canker ◽  
Filamentous Phage ◽  
Xanthomonas Citri ◽  
Canker Disease ◽  
Dynamic Integration ◽  
Citrus Canker Disease

Bacteria from the citrus phylloplane can disrupt cell-cell signalling inXanthomonas citriand reduce citrus canker disease severity

2015 ◽  
Vol 65 (5) ◽  
pp. 782-791 ◽  
Author(s):  
J. C. Caicedo ◽  
S. Villamizar ◽  
M. I. T. Ferro ◽  
K. C. Kupper ◽  
J. A. Ferro
Keyword(s):  
Disease Severity ◽  
Cell Signalling ◽  
Citrus Canker ◽  
Canker Disease ◽  
Citrus Canker Disease ◽  
Cell Cell

scholarly journals GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Simone Cristina Picchi ◽  
Mariana de Souza e Silva ◽  
Luiz Leonardo Saldanha ◽  
Henrique Ferreira ◽  
Marco Aurélio Takita ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Plant Pathogen ◽  
Model Organism ◽  
Citrus Canker ◽  
Bacterial Cells ◽  
Xanthomonas Citri ◽  
Canker Disease ◽  
Potential Use

AbstractN-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation.

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