scholarly journals Novel Erwinia persicina Infecting Phage Midgardsormr38 Within the Context of Temperate Erwinia Phages

2020 ◽  
Vol 11 ◽  
Author(s):  
Nikita Zrelovs ◽  
Andris Dislers ◽  
Andris Kazaks
Keyword(s):  
Erwinia Persicina

Characterization and Control of Erwinia spp. and Pluralibacter sp. in Tuna Salad Preparations

2019 ◽  
Vol 82 (6) ◽  
pp. 1071-1081
Author(s):  
KRISTIN BJORNSDOTTIR-BUTLER ◽  
SUSAN McCARTHY ◽  
RONALD A. BENNER
Keyword(s):  
Growth Conditions ◽  
Whole Genome ◽  
Processing Conditions ◽  
Optimum Growth ◽  
Significant Growth ◽  
Ph Values ◽  
Histamine Production ◽  
Different Temperatures ◽  
And Control ◽  
Erwinia Persicina

ABSTRACT Histamine-producing Erwinia and Pluralibacter spp. capable of producing toxic histamine levels were isolated from ingredients commonly used in tuna salad preparations. The characterization and control of these histamine-producing bacteria are necessary to prevent illness from tuna salad consumption. We confirmed the identity of two Erwinia spp. and one Pluralibacter sp. previously isolated from tuna salad ingredients through whole genome sequencing and phylogenic analysis and characterized them for growth and histamine production at different temperatures, pH values, and salt concentrations. In addition, we examined the effects of dried vinegar (DV) powder on growth and histamine production of these strains in inoculated tuna salad preparations. Optimum growth temperatures in tryptic soy broth (TSB) for the two Erwinia spp. and one Pluralibacter sp. were 30.1, 31.1, and 33.9°C, respectively, and growth in TSB was observed at 5°C for both genera. Optimum histamine production of Erwinia persicina, Erwinia spp., and Pluralibacter spp. in TSB with histidine occurred from 25 to 30°C, pH 4 to 6, and 0 to 4% NaCl. No significant growth or histamine production was observed in tuna salad preparations stored at 4°C. Growth and histamine production by Erwinia or Pluralibacter spp. was inhibited in tuna salad containing celery and onion and 2% DV, whereas significant growth and histamine production occurred in tuna salad without DV. Understanding optimum growth conditions and histamine production can provide guidance to tuna salad manufacturers in formulating products and adjusting processing conditions that minimize hazards from these histamine-producing bacteria. Addition of 2% DV to tuna salad preparations may prevent histamine production in the event of temperature abuse. HIGHLIGHTS

scholarly journals First Report of a Garlic Bulb Rot Caused by Erwinia persicina in Europe

Plant Disease ◽  
2015 ◽  
Vol 99 (5) ◽  
pp. 723 ◽  
Author(s):  
L. Gálvez ◽  
J. Gil-Serna ◽  
M. García-Díaz ◽  
D. Palmero
Keyword(s):  
First Report ◽  
Garlic Bulb ◽  
Bulb Rot ◽  
Erwinia Persicina

Structural studies on the exopolysaccharide from Erwinia persicina

2005 ◽  
Vol 340 (11) ◽  
pp. 1761-1765 ◽  
Author(s):  
Peggy Kiessling ◽  
Sof’ya N. Senchenkova ◽  
Michael Ramm ◽  
Yuriy A. Knirel
Keyword(s):  
Structural Studies ◽  
Erwinia Persicina

Pink seed of barley caused by Erwinia persicina

2021 ◽  
Author(s):  
Akira Kawaguchi ◽  
Daigo Abe ◽  
Takeshi Saito ◽  
Yoichi Nogata ◽  
Koji Nomiyama ◽  
...  
Keyword(s):  
Erwinia Persicina

scholarly journals First Report of Erwinia persicina Causing Pink Disease in Flammulina velutipes (Enoki Mushroom) in China

Plant Disease ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 1014-1014 ◽  
Author(s):  
J. J. Yan ◽  
Z. Y. Lin ◽  
R. Q. Wang ◽  
F. Liu ◽  
Z. J. Tong ◽  
...  
Keyword(s):  
Flammulina Velutipes ◽  
First Report ◽  
Erwinia Persicina ◽  
Enoki Mushroom

Experimental infection in mice with Erwinia persicina

2019 ◽  
Vol 130 ◽  
pp. 38-43
Author(s):  
Walaa I. Mohamaden ◽  
Zhang Zhen-fen ◽  
Ibrahim M. Hegab ◽  
Shi Shang-li
Keyword(s):  
Experimental Infection ◽  
Erwinia Persicina

scholarly journals Erwinia persicina Causing Chlorosis and Necrotic Spots in Leaves and Tendrils of Pisum sativum in Southeastern Spain

Plant Disease ◽  
2007 ◽  
Vol 91 (4) ◽  
pp. 460-460 ◽  
Author(s):  
A. J. González ◽  
J. C. Tello ◽  
M. R. Rodicio
Keyword(s):  
Pisum Sativum ◽  
Pcr Amplification ◽  
The United States ◽  
Biochemical Tests ◽  
Gene Encoding ◽  
Transparent Plastic ◽  
Southeastern Spain ◽  
Plastic Bags ◽  
Hydrolysis Of ◽  
Erwinia Persicina

In 2003, symptoms of generalized chlorosis as well as necrosis in leaves and tendrils were observed in Pisum sativum L. cv Tirabeque grown in green fields in southeastern Spain (Granada Province), and by 2004, the disease affected approximately 12 ha. Bacteria isolated from symptomatic samples were gram negative, rod shaped, motile, oxidase negative, facultatively anaerobic, and fermentative, which coincided with the general characteristics of the family Enterobacteriaceae. The gene encoding the 16S rRNA from two isolates (LPPA 406 and LPPA 408) was sequenced after PCR amplification (1). The two sequences were identical (EMBL Accession No. AM294946 for LPPA 408) and showed 99% similarity with several strains of Erwinia persicina (including the type strain ATCC 35998, LPPA 373, LMG 11254, GS04, and LMG 2691). Additional biochemical tests were performed using E. persicina ATCC 49742 as a control. The three strains were negative for arginine dihydrolase activity, indol production, hydrolysis of casein, and hydrolysis of gelatin. In contrast, they were positive for assimilation of adonitol, l-lactate, mannitol, m-inositol, erythritol, sorbitol, sucrose, nitrate reduction, hydrolysis of aesculin, and growth in 5% NaCl at 36°C. Nevertheless, E. persicina ATTC 49742, but not the isolates from P. sativum, produced a pink pigment. The latter isolates were also tested for pathogenicity. Bacterial suspensions (108 CFU/ml) were spray inoculated on 10 pea seedlings of cv. Tirabeque. Seedlings were covered with transparent plastic bags for 2 days and held in an incubation chamber at 22°C and 80% relative humidity with a 12-h photoperiod. Assays were conducted twice. Symptoms that developed were similar to those originally observed in the field, whereas symptoms did not occur on control seedlings sprayed with sterile distilled water. Bacteria sharing the characteristics of the inoculated isolates were recovered from symptomatic plants, hence fulfilling Koch's postulates. E. persicina has been isolated previously from bean in the United States (3) and southeastern Spain (1) and from tomato, banana, and cucumber in Japan (2). To our knowledge, this is the first report of the bacterium on P. sativum. References: (1) A. J. González et al. Plant Dis. 89:109, 2005. (2) M. V. Hao et al. Int. J. Syst. Bacteriol. 40:379, 1990. (3) M. L. Schuster et al. Fitopatol. Bras. 6:345, 1990.

scholarly journals First Report of Bacterial Leaf Spot of Cucurbita pepo Caused by Erwinia persicina in China

Plant Disease ◽  
2020 ◽  
Author(s):  
Lei Li ◽  
Huanling Li ◽  
Yanxia Shi ◽  
A LI CHAI ◽  
Xuewen Xie ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Leaf Spot ◽  
Cucurbita Pepo ◽  
Housekeeping Genes ◽  
Leaf Spot Disease ◽  
Bacterial Isolates ◽  
First Report ◽  
Initial Symptoms ◽  
Erwinia Persicina

In February 2020, the common symptoms of water-soaked spots on Cucurbita pepo L. cotyledon were observed in Guangrao county in Shandong province, China. Field investigation showed that 40% of the Cucurbita pepo cotyledons in an area of approximately 0.8 ha were infected. The disease resulted in a severe loss in seedling production. Samples of C. pepo with water-soaked leaf spots were collected and prepared for pathogen analysis. Symptomatic cotyledon tissue was surface disinfested in 75% ethanol for 30 sec, then rinsed three times in sterilized water. Bacteria were released in sterile water in Petri dish for 2 min by cutting symptomatic tissue into small sections and stirring the plant tissue mixture fully. The diffusate was streaked onto plates containing nutrient agar (NA) and plates were incubated at 28℃ for 2 days. Three representative isolates were purified eventually from each of the plates. Colonies on NA were small, round and with smooth margins. All bacterial isolates characterized as gram-negative, white to cream color, and pink pigment was formed on the plates over long-term culture. The isolates were positive for catalase, Voges­Proskauer, potato rot, methyl red, acetoin production, nitrate utilization and citrate utilization, and acid production from maltose, glucose, melezitose, sucrose, D-arabinose, D-trehalose, cellobiose, lactose, raffinose, mannitol, D-sorbitol, melibiose and xylitol. KOH production was demonstrated according to strand formation within the potassium hydroxide test (Suslow et al. 1982). Isolates were negative for oxidase, arginine dihydrolase, phenylalanine deaminase, gelatinase, esculine, indole production and H2S production. Total genomic DNA was extracted from isolate XHL2002230201 with TIANamp Bacteria DNA Kit (TIANGEN). Universal primers 27F and 1492R (Monciardini et al. 2002) were used in PCR to amplify a 1,307-bp DNA fragment of the 16S rRNA region for molecular identification. Furthermore, four additional housekeeping genes (gyrB, atpD, rho, and rpoS) were selected and amplified using specially designed primers. The amplification products of 16S rRNA were sequenced and submitted to GenBank under accession number (MT568607.1). Sequence analysis showed 99% similarity to Erwinia persicina strains B57 (LM651373.1) and B64 (CI789_17875) by BLAST search in GenBank database (Gálvez et al. 2015; Cho et al. 2019). A phylogenetic tree was constructed, and the taxonomic position of strain XHL2002230201 was determined from the multilocus sequence analysis (MLSA) on 16S rRNA and other four housekeeping genes with E. persicina and not with other closely related Erwinia species. Pathogenicity tests and re-isolation and re-identification of the bacteria were performed to confirm the isolate and fulfill the Koch' postulates. The strain XHL2002230201 suspensions (108 CFU ml−1) were spray inoculated onto fifteen Cucurbita pepo seedlings with two true leaves, and the same number of control plants were inoculated with water. Experiments were repeated three times. All inoculated plants were kept in a moist chamber placed in a greenhouse at 28℃. Initial symptoms were observed on leaves of inoculated plants at 5 days post-inoculation, whereas no symptoms appeared on the plants inoculated with sterile distilled water. Based on morphological and biochemical characteristics, phylogenetic analysis, and Koch's postulates, the bacterial isolates were identified as E. persicina. To our knowledge, this is the first report of E. persicina causing leaf spot disease on Cucurbita pepo in China.

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