scholarly journals Formation and Control of the Viable but Non-culturable State of Foodborne Pathogen Escherichia coli O157:H7

2020 ◽  
Vol 11 ◽  
Author(s):  
Yanmei Li ◽  
Teng-Yi Huang ◽  
Congxiu Ye ◽  
Ling Chen ◽  
Yi Liang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Escherichia Coli O157 ◽  
And Control

Inactivation of Escherichia coli O157:H7 in Cattle Drinking Water by Sodium Caprylate

2006 ◽  
Vol 69 (9) ◽  
pp. 2248-2252 ◽  
Author(s):  
MARY ANNE ROSHNI AMALARADJOU ◽  
THIRUNAVUKKARASU ANNAMALAI ◽  
PATRICK MAREK ◽  
PEDRAM REZAMAND ◽  
DAVID SCHREIBER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Storage Temperature ◽  
Foodborne Pathogen ◽  
Escherichia Coli O157 ◽  
Environmental Persistence ◽  
E Coli ◽  
Sodium Caprylate ◽  
And Control

Escherichia coli O157:H7 is an important foodborne pathogen. Cattle serve as one of the major reservoirs of E. coli O157:H7, excreting the pathogen in feces. Environmental persistence of E. coli O157:H7 is critical in its epidemiology on far MS, and the pathogen has been isolated from cattle water troughs. Thus, there is a need for an effective method for killing E. coli O157:H7 in cattle drinking water. In this study, the efficacy of sodium caprylate for killing E. coli O157:H7 in cattle drinking water was investigated. A four-strain mixture of E. coli O157:H7 was inoculated (6.0 log CFU/ml) into 100-ml samples of well water containing 0, 75, 100, or 120 mM sodium caprylate. Water samples containing 1% (wt/vol) bovine feces or feed also were included. The samples were incubated at 21 or 8°C for 21 days. Water samples were analyzed for viable E. coli O157:H7 on days 0, 1, 3, 5, and 7 and weekly thereafter. Triplicate samples of each treatment and control were included, and the study was repeated twice. The magnitude of E. coli O157:H7 inactivation in water significantly increased (P < 0.01) with increases in caprylate concentration and storage temperature. At 120 mM, sodium caprylate completely inactivated E. coli O157:H7 in all the samples after 1 to 20 days, depending on the treatments. The presence of feces or feed also had a significant effect (P < 0.01) on the antibacterial property of caprylate; the presence of feces decreased the antibacterial effect, whereas addition of feed enhanced the effect. These results indicate that sodium caprylate is effective in killing E. coli O157:H7 in cattle drinking water, but detailed cattle palatability studies of water containing caprylate are necessary.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep

2009 ◽  
Vol 89 (2) ◽  
pp. 285-293 ◽  
Author(s):  
S J Bach ◽  
R P Johnson ◽  
K. Stanford ◽  
T A McAllister
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Total Coliforms ◽  
E Coli ◽  
Oral Inoculation ◽  
Experimental Treatments ◽  
And Control

Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages (phage), and control (CON). Oral inoculation of the rams with 109 CFU of a mixture of four nalidixic acid-resistant strains of E. coli O157:H7 was performed on day 0. A mixture of 1010 PFU of bacteriophages P5, P8 and P11 was administered on days -2, -1, 0, 6 and 7. Fecal samples collected on 14 occasions over 21 d were analyzed for E. coli O157:H7, total E. coli, total coliforms and bacteriophages. Sheep in treatment O157+phage shed fewer (P < 0.05) E. coli O157:H7 than did sheep in treatment O157. Populations of total coliforms and total E. coli were similar (P < 0.05) among treatments, implying that bacteriophage lysis of non-target E. coli and coliform bacteria in the gastrointestinal tract did not occur. Bacteriophage numbers declined rapidly over 21 d, which likely reduced the chance of collision between bacteria and bacteriophage. Oral administration of bacteriophages reduced shedding of E. coli O157:H7 by sheep, but a delivery system that would protect bacteriophages during passage through the intestine may increase the effectiveness of this strategy as well as allow phage to be administered in the feed.Key words: Escherichia coli O157:H7, bacteriophage, sheep, environment, coliforms

A Comparison of the Survival in Feces and Water of Escherichia coli O157:H7 Grown under Laboratory Conditions or Obtained from Cattle Feces

2006 ◽  
Vol 69 (1) ◽  
pp. 6-11 ◽  
Author(s):  
L. SCOTT ◽  
P. McGEE ◽  
J. J. SHERIDAN ◽  
B. EARLEY ◽  
N. LEONARD
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Hemorrhagic Colitis ◽  
Contaminated Water ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Cattle Feces ◽  
Oral Inoculation ◽  
Before And After ◽  
Uremic Syndrome

Escherichia coli O157:H7 is an important foodborne pathogen that can cause hemorrhagic colitis and hemolytic uremic syndrome. Cattle feces and fecally contaminated water are important in the transmission of this organism on the farm. In this study, the survival of E. coli O157:H7 in feces and water was compared following passage through the animal digestive tract or preparation in the laboratory. Feces were collected from steers before and after oral inoculation with a marked strain of E. coli O157:H7. Fecal samples collected before cattle inoculation were subsequently inoculated with the marked strain of E. coli O157:H7 prepared in the laboratory. Subsamples were taken from both animal and laboratory-inoculated feces to inoculate 5-liter volumes of water. E. coli O157:H7 in feces survived up to 97 days, and survival was not affected by the method used to prepare the inoculating strain. E. coli O157:H7 survived up to 109 days in water, and the bacteria collected from inoculated cattle were detected up to 10 weeks longer than the laboratory-prepared culture. This study suggests that pathogen survival in low-nutrient conditions may be enhanced by passage through the gastrointestinal tract.

Rapid and accurate detection of Escherichia coli O157:H7 in beef using microfluidic wax-printed paper-based ELISA

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3106-3115 ◽  
Author(s):  
Yanan Zhao ◽  
Dexin Zeng ◽  
Chao Yan ◽  
Wei Chen ◽  
Jianluan Ren ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Point Of Care ◽  
Foodborne Pathogen ◽  
Escherichia Coli O157 ◽  
Good Sensitivity ◽  
Point Of Care Testing ◽  
Accurate Detection ◽  
New Ideas ◽  
Sensitivity Specificity

Escherichia coli O157:H7 is a severe foodborne pathogen. Paper-based ELISA can rapidly and accurately detect E.coli O157:H7 in beef. The method has good sensitivity, specificity and repeatability. It is suitable for point-of-care testing and offers new ideas for the detection of other foodborne pathogens.

Incidence and Toxin Production Ability of Escherichia coli O157:H7 Isolated from Cattle Trucks†

2007 ◽  
Vol 70 (10) ◽  
pp. 2383-2385 ◽  
Author(s):  
E. P. CUESTA ALONSO ◽  
S. E. GILLILAND ◽  
C. R. KREHBIEL
Keyword(s):  
Escherichia Coli ◽  
Potential Risk ◽  
Foodborne Pathogen ◽  
Toxin Production ◽  
Escherichia Coli O157 ◽  
Foodborne Illnesses ◽  
E Coli

Twelve cattle trucks were analyzed for the presence of Escherichia coli O157:H7. Three of them had been washed prior to arrival, and the others had not. Seventy-five percent of the trailers were positive for the presence of this foodborne pathogen. A total of 54 cultures were isolated and identified as E. coli O157:H7, all from the trucks that had not been cleaned. Most of the cultures (96.4%) produced Shiga-like toxin (verotoxin). No E. coli O157:H7 was detected in cattle trucks that were cleaned before arrival at the cattle pens. The incidence of E. coli O157:H7 in transport trailers increases the potential risk of contamination of cattle and transmission from farms to feedlots and to packing plants. This contamination increases the potential of contamination of meat during harvest and the risk of foodborne illnesses.

Growth of Escherichia coli O157:H7 at Fluctuating Incubation Temperatures

1995 ◽  
Vol 58 (12) ◽  
pp. 1307-1313 ◽  
Author(s):  
KATHLEEN T. RAJKOWSKI ◽  
BENNE S. MARMER
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Brain Heart Infusion ◽  
Growth Curves ◽  
Escherichia Coli O157 ◽  
Growth Responses ◽  
Growth Data ◽  
Fluctuating Temperature ◽  
E Coli ◽  
Fluctuating Temperatures

Temperature abuse of foods is often transitory and little information is available describing the response of the foodborne pathogen, Escherichia coli O157:H7, to nonisothermal and/or fluctuating temperature storage. Growth responses were determined for a mixture of three E. coli O157:H7 strains in brain heart infusion (BHI) broth as a function of temperature (static and fluctuating), initial pH (5, 6, and 7), and NaCl content (0.5, 1, 2, and 3%). Five 6-h “square-wave” fluctuating temperature regimes were used: 4 to 12, 4 to 19, 4 to 28, 8 to 19, and 12 to 28°C and compared with growth at 8, 10, 12, 19, and 28°C. The growth curves obtained from fitting the Gompertz equation for the fluctuating temperatures were compared to those obtained for the static temperatures. Increased NaCl concentration decreased growth temperature both for the fluctuating temperature growth curves and the static growth data. The cells grew or remained viable for up to 21 days under all conditions and fluctuating temperatures. Growth kinetics at fluctuating temperatures more closely approximated the higher temperature than the midpoint temperature of each cyclic range. The results indicate that transitory abuse could lead to more rapid growth than expected of E. coli O157:H7 in foods and that given sufficient time E. coli O157:H7 can grow at as low as 8°C.

Escherichia coli O157:H7 Prevalence in Fecal Samples of Cattle from a Southeastern Beef Cow-Calf Herd

2003 ◽  
Vol 66 (10) ◽  
pp. 1778-1782 ◽  
Author(s):  
D. G. RILEY ◽  
J. T. GRAY ◽  
G. H. LONERAGAN ◽  
K. S. BARLING ◽  
C. C. CHASE
Keyword(s):  
Escherichia Coli ◽  
Control Point ◽  
Foodborne Pathogen ◽  
Beef Cows ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
E Coli ◽  
Critical Control Point ◽  
Cow Calf ◽  
Culture Positive

The proportion of fecal samples culture-positive for Escherichia coli O157:H7 was determined for samples collected from 296 beef cows on pasture in a single Florida herd in October, November, and December 2001. The overall proportion of samples that cultured positive was 0.03. The proportion of cows that were culture-positive on at least one occasion was 0.091. No effect of pregnancy status or nutritional regimen on the proportion of culture-positive samples for E. coli O157:H7 was detected. We detected a breed effect on the shedding of E. coli O157, with Romosinuano cows having a lower (P &lt; 0.01) proportion of samples culture-positive than Angus or Brahman cows. This difference might have resulted from the presence of confounding variables; however, it also might represent evidence of breed-to-breed genetic variation in E. coli O157 shedding. Further research is warranted to evaluate breed as a possible risk factor for shedding of this important foodborne pathogen. Further substantiated findings could indicate that breed is a cow-calf–level critical control point of E. coli O157:H7.

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