scholarly journals Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix

2020 ◽  
Vol 11 ◽  
Author(s):  
Paul Telengech ◽  
Sakae Hisano ◽  
Cyrus Mugambi ◽  
Kiwamu Hyodo ◽  
Juan Manuel Arjona-López ◽  
...  
Keyword(s):  
Phytopathogenic Fungus ◽  
Rosellinia Necatrix

scholarly journals A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing

2013 ◽  
Vol 87 (12) ◽  
pp. 6727-6738 ◽  
Author(s):  
S. Chiba ◽  
Y.-H. Lin ◽  
H. Kondo ◽  
S. Kanematsu ◽  
N. Suzuki
Keyword(s):  
Rna Silencing ◽  
Phytopathogenic Fungus ◽  
Rosellinia Necatrix

Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus,Rosellinia necatrix: insights into evolutionary biology of fungal viruses

2018 ◽  
Vol 20 (4) ◽  
pp. 1464-1483 ◽  
Author(s):  
Juan Manuel Arjona-Lopez ◽  
Paul Telengech ◽  
Atif Jamal ◽  
Sakae Hisano ◽  
Hideki Kondo ◽  
...  
Keyword(s):  
Evolutionary Biology ◽  
Rna Viruses ◽  
Phytopathogenic Fungus ◽  
Rosellinia Necatrix ◽  
Fungal Viruses

scholarly journals Differential Inductions of RNA Silencing among Encapsidated Double-Stranded RNA Mycoviruses in the White Root Rot Fungus Rosellinia necatrix

2016 ◽  
Vol 90 (12) ◽  
pp. 5677-5692 ◽  
Author(s):  
Hajime Yaegashi ◽  
Takeo Shimizu ◽  
Tsutae Ito ◽  
Satoko Kanematsu
Keyword(s):  
Rna Silencing ◽  
Defense Mechanism ◽  
Fluorescent Protein ◽  
Constitutive Expression ◽  
Phytopathogenic Fungus ◽  
Rosellinia Necatrix ◽  
Double Stranded Rna ◽  
Content Type ◽  
Molecular Features ◽  
Fungal Viruses

ABSTRACTRNA silencing acts as a defense mechanism against virus infection in a wide variety of organisms. Here, we investigated inductions of RNA silencing against encapsidated double-stranded RNA (dsRNA) fungal viruses (mycoviruses), including a partitivirus (RnPV1), a quadrivirus (RnQV1), a victorivirus (RnVV1), a mycoreovirus (RnMyRV3), and a megabirnavirus (RnMBV1) in the phytopathogenic fungusRosellinia necatrix. Expression profiling of RNA silencing-related genes revealed that a dicer-like gene, an Argonaute-like gene, and two RNA-dependent RNA polymerase genes were upregulated by RnMyRV3 or RnMBV1 infection but not by other virus infections or by constitutive expression of dsRNA inR. necatrix. Massive analysis of viral small RNAs (vsRNAs) from the five mycoviruses showed that 19- to 22-nucleotide (nt) vsRNAs were predominant; however, their ability to form duplexes with 3′ overhangs and the 5′ nucleotide preferences of vsRNAs differed among the five mycoviruses. The abundances of 19- to 22-nt vsRNAs from RnPV1, RnQV1, RnVV1, RnMyRV3, and RnMBV1 were 6.8%, 1.2%, 0.3%, 13.0%, and 24.9%, respectively. Importantly, the vsRNA abundances and accumulation levels of viral RNA were not always correlated, and the origins of the vsRNAs were distinguishable among the five mycoviruses. These data corroborated diverse interactions between encapsidated dsRNA mycoviruses and RNA silencing. Moreover, a green fluorescent protein (GFP)-based sensor assay inR. necatrixrevealed that RnMBV1 infection induced silencing of the target sensor gene (GFP gene and the partial RnMBV1 sequence), suggesting that vsRNAs from RnMBV1 activated the RNA-induced silencing complex. Overall, this study provides insights into RNA silencing against encapsidated dsRNA mycoviruses.IMPORTANCEEncapsidated dsRNA fungal viruses (mycoviruses) are believed to replicate inside their virions; therefore, there is a question of whether they induce RNA silencing. Here, we investigated inductions of RNA silencing against encapsidated dsRNA mycoviruses (a partitivirus, a quadrivirus, a victorivirus, a mycoreovirus, and a megabirnavirus) inRosellinia necatrix. We revealed upregulation of RNA silencing-related genes inR. necatrixinfected with a mycoreovirus or a megabirnavirus but not with other viruses, which was consistent with the relatively high abundances of vsRNAs from the two mycoviruses. We also showed common and different molecular features and origins of the vsRNAs from the five mycoviruses. Furthermore, we demonstrated the activation of RNA-induced silencing complex by mycoviruses inR. necatrix. Taken together, our data provide insights into an RNA silencing pathway against encapsidated dsRNA mycoviruses which is differentially induced among encapsidated dsRNA mycoviruses; that is, diverse replication strategies exist among encapsidated dsRNA mycoviruses.

scholarly journals The Rhizobacterium Pseudomonas alcaligenes AVO110 Induces the Expression of Biofilm-Related Genes in Response to Rosellinia necatrix Exudates

2021 ◽  
Vol 9 (7) ◽  
pp. 1388
Author(s):  
Adrián Pintado ◽  
Isabel Pérez-Martínez ◽  
Isabel M. Aragón ◽  
José Antonio Gutiérrez-Barranquero ◽  
Antonio de Vicente ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Variable Region ◽  
Phytopathogenic Fungus ◽  
Gene Pools ◽  
Rosellinia Necatrix ◽  
Environmental Isolates ◽  
Pseudomonas Alcaligenes ◽  
Domain Protein ◽  
Genome Analyses ◽  
Soil And Water

The rhizobacterium Pseudomonas alcaligenes AVO110 exhibits antagonism toward the phytopathogenic fungus Rosellinia necatrix. This strain efficiently colonizes R. necatrix hyphae and is able to feed on their exudates. Here, we report the complete genome sequence of P. alcaligenes AVO110. The phylogeny of all available P. alcaligenes genomes separates environmental isolates, including AVO110, from those obtained from infected human blood and oyster tissues, which cluster together with Pseudomonas otitidis. Core and pan-genome analyses showed that P. alcaligenes strains encode highly heterogenic gene pools, with the AVO110 genome encoding the largest and most exclusive variable region (~1.6 Mb, 1795 genes). The AVO110 singletons include a wide repertoire of genes related to biofilm formation, several of which are transcriptionally modulated by R. necatrix exudates. One of these genes (cmpA) encodes a GGDEF/EAL domain protein specific to Pseudomonas spp. strains isolated primarily from the rhizosphere of diverse plants, but also from soil and water samples. We also show that CmpA has a role in biofilm formation and that the integrity of its EAL domain is involved in this function. This study contributes to a better understanding of the niche-specific adaptations and lifestyles of P. alcaligenes, including the mycophagous behavior of strain AVO110.

A novel totivirus isolated from the phytopathogenic fungus Rhodosporidiobolus odoratus strain GZ2017

2020 ◽  
Vol 165 (8) ◽  
pp. 1911-1914
Author(s):  
Tingting Zhang ◽  
Xiaoyao Cai ◽  
Zhu Zeng ◽  
Hang Du ◽  
Hongmei Liu
Keyword(s):  
Phytopathogenic Fungus

Self- and nonself recognition during hyphal interactions in Rosellinia necatrix

2015 ◽  
Vol 81 (6) ◽  
pp. 420-428 ◽  
Author(s):  
Takahiro Uwamori ◽  
Kanako Inoue ◽  
Chiaki Kida ◽  
Yuichi Morita ◽  
Pyoyun Park ◽  
...  
Keyword(s):  
Rosellinia Necatrix ◽  
Nonself Recognition
Sign in / Sign up

Export Citation Format

Share Document