scholarly journals Characterization of Calcium-Dependent Protein Kinases 3, a Protein Involved in Growth of Cryptosporidium parvum

2020 ◽  
Vol 11 ◽  
Author(s):  
Qiang Zhang ◽  
Yaqiong Guo ◽  
Na Li ◽  
Yu Li ◽  
Jiayuan Su ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Cryptosporidium Parvum ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

scholarly journals Characterization of Three Calcium-Dependent Protein Kinases of Cryptosporidium parvum

2021 ◽  
Vol 11 ◽  
Author(s):  
Qiang Zhang ◽  
Qian Shao ◽  
Yaqiong Guo ◽  
Na Li ◽  
Yu Li ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Cryptosporidium Parvum ◽  
Protein Localization ◽  
Neutralization Assay ◽  
Gene Expressions ◽  
Calcium Dependent ◽  
Reverse Transcription Pcr ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

In Cryptosporidium spp., calcium-dependent protein kinases (CDPKs) are considered promising targets for the development of pharmaceutical interventions. Whole-genome sequencing has revealed the presence of 11 CDPKs in Cryptosporidium parvum (CpCDPKs). In this study, we expressed recombinant CpCDPK4, CpCDPK5, and CpCDPK6 in Escherichia coli. The biological characteristics and functions of these CpCDPKs were examined by using quantitative reverse transcription PCR (qRT-PCR), immunofluorescence microscopy, and an in vitro neutralization assay. The expression of the CpCDPK4 gene peaked at 12 h post-infection, the CpCDPK5 gene peaked at 12 and 48 h, and the CpCDPK6 gene peaked at 2–6 h. CpCDPK4 protein was located in the anterior and mid-anterior regions of sporozoites, and CpCDPK5 protein was located over the entire sporozoites, while CpCDPK6 protein was expressed in a spotty pattern. Immune sera of CpCDPK4 and CpCDPK6 exhibited significant inhibitory effects on host cell invasion, while the immune sera of CpCDPK5 had no effects. These differences in protein localization, gene expressions, and neutralizing capacities indicated that the CpCDPK proteins may have different roles during the lifecycle of Cryptosporidium spp.

Calcium-Dependent Protein Kinases (CDPK) in Abiotic Stress Tolerance

2018 ◽  
Vol 34 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Hemant B Kardile ◽  
◽  
Vikrant ◽  
Nirmal Kant Sharma ◽  
Ankita Sharma ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Kinases ◽  
Abiotic Stress Tolerance ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases
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