scholarly journals Identification of Secreted O-Mannosylated Proteins From BCG and Characterization of Immunodominant Antigens BCG_0470 and BCG_0980

2020 ◽  
Vol 11 ◽  
Author(s):  
Guoying Deng ◽  
Wenli Zhang ◽  
Na Ji ◽  
Yunpeng Zhai ◽  
Xiaoxia Shi ◽  
...  
Keyword(s):  
Immunodominant Antigens

Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)

Parasitology ◽  
1998 ◽  
Vol 117 (4) ◽  
pp. 321-330 ◽  
Author(s):  
R. A. SKILTON ◽  
R. P. BISHOP ◽  
C. W. WELLS ◽  
P. R. SPOONER ◽  
E. GOBRIGHT ◽  
...  
Keyword(s):  
Immunoelectron Microscopy ◽  
Signal Sequence ◽  
Peptide Sequence ◽  
Theileria Parva ◽  
Repeat Region ◽  
Gene Encoding ◽  
Genes Encoding ◽  
Immunodominant Antigens ◽  
Proline Rich Region

To identify the genes encoding novel immunodominant antigens of Theileria parva a λgt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal sequence and a proline-rich region of repeated amino acid motifs. The repeat region is polymorphic between stocks of T. parva in both copy number and sequence, and analysis of the repeat region from 10 stocks of T. parva revealed 5 p150 variants. A monoclonal antibody (mAb) against the T. parva polymorphic immunodominant molecule (PIM) cross-reacted with the recombinant p150. The p150 has sequence homology with a PIM peptide sequence containing the anti-PIM mAb epitope. Immunoelectron microscopy demonstrated that the p150 antigen, like PIM, is located in the microspheres of the sporozoites and is exocytosed following sporozoite invasion of the host lymphocyte. By immunoelectron microscopy p150 was subsequently transiently detectable on the sporozoite surface and in the lymphocyte cytosol. Immunoblotting showed that p150 is also expressed by the schizont stage, but at much lower levels compared to the sporozoite. These results suggest a major role for p150 in the early events of host–sporozoite interaction.

Characterization of variable immunodominant antigens of Cowdria ruminantium by ELISA and immunoblots

1998 ◽  
Vol 20 (12) ◽  
pp. 613-622 ◽  
Author(s):  
JEAN-MARIE PEREZ ◽  
DOMINIQUE MARTINEZ ◽  
CHRISTIAN SHEIKBOUDOU ◽  
FRANS JONGEJAN ◽  
ALBERT BENSAID
Keyword(s):  
Cowdria Ruminantium ◽  
Immunodominant Antigens

scholarly journals Characterization of novel immunodominant antigens of Mycobacterium tuberculosis

Microbiology ◽  
1998 ◽  
Vol 144 (5) ◽  
pp. 1197-1203 ◽  
Author(s):  
R. R. Amara ◽  
S. Shanti ◽  
V. Satchidanandam
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Immunodominant Antigens

scholarly journals Identification and Characterization of Immunodominant Antigens during the Course of Infection with Brucella Ovis

1995 ◽  
Vol 7 (2) ◽  
pp. 210-218 ◽  
Author(s):  
Reinhold Kittelberger ◽  
Frans Hilbink ◽  
Mike F. Hansen ◽  
Gail P. Ross ◽  
Geoffrey W. de Lisle ◽  
...  
Keyword(s):  
False Positive ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Outer Membrane Proteins ◽  
Infected Sheep ◽  
Infected Group ◽  
Brucella Ovis ◽  
Immunodominant Antigens ◽  
Identification And Characterization

The seroresponse against Brucella ovis of 8 intrapreputially and 6 intravenously infected rams and 9 ewes infected through mating was analyzed by electrophoretic immunoblotting. Additionally, 87 sera from chronically infected rams that were shedding B. ovis in their semen, 226 sera from rams belonging to infected flocks, and 324 sera from false-positive complement fixation test (CFT) reactors were examined. In all infected animals, antibody reactivity was predominantly found against 5 B. ovis components of 8–12, 17, 19, 29, and 63 kD, of which the 29-kD antigen was most dominant in the seroresponse. Antibodies to the 29-kD component were present in 93-100% of the infected sheep in each infected group, whereas the frequency of antibodies to the 4 other components varied considerably among and within the different groups. No reactivity against the 29-kD antigen was found in the false-positive CFT reactors. By using monoclonal antibodies against known bacterial macromolecules, the immunodominant antigens were identified as rough lipopolysaccharide (8-12 kD), outer membrane proteins (17, 19, 29 kD), and a heat-shock protein (63 kD).

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

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