scholarly journals The Impacts of Reassortant Avian Influenza H5N2 Virus NS1 Proteins on Viral Compatibility and Regulation of Immune Responses

2020 ◽  
Vol 11 ◽  
Author(s):  
Wen-Chien Wang ◽  
Chih-Ying Kuan ◽  
Yu-Jing Tseng ◽  
Chia-Hsuan Chang ◽  
Yee-Chen Liu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Immune Responses ◽  
H5n2 Virus

Vaccination with CpG-Adjuvanted Avian Influenza Virosomes Promotes Antiviral Immune Responses and Reduces Virus Shedding in Chickens

2012 ◽  
pp. 120418071632002 ◽  
Author(s):  
Amirul I. Mallick ◽  
Raveendra R. Kulkarni ◽  
Michael St. Paul ◽  
Payvand Parvizi ◽  
Éva Nagy ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Immune Responses ◽  
Virus Shedding

scholarly journals Cross reactive cellular immune responses in chickens previously exposed to low pathogenic avian influenza

2011 ◽  
Vol 5 (S4) ◽  
Author(s):  
Darrell R Kapczynski ◽  
Karen Liljebjelke ◽  
Gururaj Kulkarni ◽  
Henry Hunt ◽  
Hai Jun Jiang ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Immune Responses ◽  
Cellular Immune Responses ◽  
Pathogenic Avian Influenza ◽  
Low Pathogenic Avian Influenza ◽  
Cellular Immune

scholarly journals Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses

Drug Delivery ◽  
2018 ◽  
Vol 25 (1) ◽  
pp. 773-779 ◽  
Author(s):  
Weiping Cao ◽  
Margarita Mishina ◽  
Samuel Amoah ◽  
Wadzanai P. Mboko ◽  
Caitlin Bohannon ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Immune Responses ◽  
Influenza Vaccine ◽  
Nasal Delivery ◽  
H5n1 Avian Influenza

Evaluation of a new viral vaccine vector in mice and rhesus macaques: J paramyxovirus (JPV)-expressing HA of influenza A virus H5N1

2021 ◽  
Author(s):  
Mathew Abraham ◽  
Ashley C. Beavis ◽  
Peng Xiao ◽  
Francois J Villinger ◽  
Zhuo Li ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Immune Responses ◽  
Respiratory Tract ◽  
Rhesus Macaques ◽  
Host Genome ◽  
Vaccine Vector ◽  
Viral Vaccine ◽  
Hpai H5n1 ◽  
Foreign Genes ◽  
H5n1 Vaccine

H5N1, an avian influenza virus, is known to circulate in many Asian countries like Bangladesh, China, Cambodia, Indonesia, and Vietnam. The current FDA-approved H5N1 vaccine has a moderate level of efficacy. A safe and effective vaccine is needed to prevent the outbreaks of highly pathogenic avian influenza (HPAI) H5N1 in humans. Non-segmented negative-sense single-stranded viruses (NNSVs) are widely used as a vector to develop vaccines for humans, animals, and poultry. NNSVs stably express foreign genes without integrating with the host genome. J Paramyxovirus (JPV) is a non-segmented negative-strand RNA virus and a member of the proposed genus Jeilongvirus in the family Paramyxoviridae . JPV-specific antibodies have been detected in rodents, bats, humans, and pigs, but the virus is not associated with disease in any species other than mice. JPV replicates in the respiratory tract of mice and efficiently expresses the virus-vectored foreign genes in tissue culture cells. In this work, we explored JPV as a vector for developing an H5N1 vaccine using intranasal delivery. We incorporated hemagglutinin (HA) of H5N1 into the JPV genome by replacing the small hydrophobic (SH) gene to generate a recombinant JPV expressing HA (rJPV-ΔSH-H5). A single intranasal administration of rJPV-ΔSH-H5 protected mice from a lethal HPAI H5N1 challenge. Intranasal vaccination of rJPV-ΔSH-H5 in rhesus macaques elicited antigen-specific humoral and cell-mediated immune responses. This work demonstrates that JPV is a promising vaccine vector. IMPORTANCE HPAI H5N1 outbreak in Southeast Asia destroyed millions of birds. Transmission of H5N1 into humans resulted in deaths in many countries. In this work, we developed a novel H5N1 vaccine candidate using JPV as a vector and demonstrated that JPV is an efficacious vaccine vector in animals. NNSVs stably express foreign genes without integrating into the host genome. JPV, an NNSV, replicates efficiently in the respiratory tract and induces robust immune responses.

scholarly journals Characterization of Host Responses against a Recombinant Fowlpox Virus-Vectored Vaccine Expressing the Hemagglutinin Antigen of an Avian Influenza Virus

2010 ◽  
Vol 17 (3) ◽  
pp. 454-463 ◽  
Author(s):  
Hamid R. Hghihghi ◽  
Leah R. Read ◽  
Hakimeh Mohammadi ◽  
Yanlong Pei ◽  
Claudia Ursprung ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Immune Responses ◽  
Avian Influenza Virus ◽  
Cultured Cells ◽  
Type I Interferons ◽  
Host Responses ◽  
Type I ◽  
Fowlpox Virus ◽  
Time Points

ABSTRACT There currently are commercial fowlpox virus (FPV)-vectored vaccines for use in chickens, including TROVAC-AIV H5, which expresses the hemagglutinin (HA) antigen of an avian influenza virus and can confer immunity against avian influenza in chickens. Despite the use of recombinant FPV (rFPV) for vaccine delivery, very little is known about the immune responses generated by these viruses in chickens. The present study was designed to investigate host responses to rFPV in vivo and in vitro. In cultured cells infected with TROVAC-AIV H5, there was an early increase in the expression of type I interferons (IFN), Toll-like receptors 3 and 7 (TLR3 and TLR7, respectively), TRIF, and MyD88, which was followed by a decrease in the expression of these genes at later time points. There also was an increase in the expression of interleukin-1β (IL-1β), IL-8, and beta-defensin genes at early time points postinfection. In chickens immunized with TROVAC-AIV H5, there was higher expression of IFN-γ and IL-10 at day 5 postvaccination in spleen of vaccinated birds than in that of control birds. We further investigated the ability of the vaccine to induce immune responses against the HA antigen and discovered that there was a cell-mediated response elicited in vaccinated chickens against this antigen. The findings of this study demonstrate that FPV-vectored vaccines can elicit a repertoire of responses marked by the early expression of TLRs, type I interferons, and proinflammatory cytokines, as well as cytokines associated with adaptive immune responses. This study provides a platform for designing future generations of rFPV-vectored vaccines.

Cross-clade protective immune responses of NS1-truncated live attenuated H5N1 avian influenza vaccines

Vaccine ◽  
2016 ◽  
Vol 34 (3) ◽  
pp. 350-357 ◽  
Author(s):  
Shaohua Shi ◽  
Sujuan Chen ◽  
Weizhou Han ◽  
Bai Wu ◽  
Xiaojian Zhang ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Immune Responses ◽  
Influenza Vaccines ◽  
H5n1 Avian Influenza
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