scholarly journals Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris

2020 ◽  
Vol 11 ◽  
Author(s):  
Jyoti Gupta ◽  
Sheetal Kaul ◽  
Akriti Srivastava ◽  
Neha Kaushik ◽  
Sukanya Ghosh ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Purification And Characterization ◽  
Virus Like Particles

P.331 Characterization of recombinant virus-like particles of genotype 3 hepatitis E virus

2006 ◽  
Vol 36 ◽  
pp. S163
Author(s):  
T. Li ◽  
H. Tsunemitsu ◽  
L. Xing ◽  
H. Cheng ◽  
N. Nagata ◽  
...  
Keyword(s):  
Recombinant Virus ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Like Particles

Production of capsid proteins of rat hepatitis E virus in Escherichia coli and characterization of self-assembled virus-like particles

2021 ◽  
pp. 198483
Author(s):  
Tominari Kobayashi ◽  
Masaharu Takahashi ◽  
Satoshi Ohta ◽  
Shigeo Nagashima ◽  
Putu Prathiwi Primadharsini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Capsid Proteins ◽  
Virus Like Particles ◽  
Self Assembled

scholarly journals Characterization of self-assembled virus-like particles of dromedary camel hepatitis e virus generated by recombinant baculoviruses

2015 ◽  
Vol 210 ◽  
pp. 8-17 ◽  
Author(s):  
Xianfeng Zhou ◽  
Michiyo Kataoka ◽  
Zheng Liu ◽  
Naokazu Takeda ◽  
Takaji Wakita ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Baculoviruses ◽  
Dromedary Camel ◽  
Virus Like Particles ◽  
Self Assembled

Robust manufacturing and comprehensive characterization of recombinant hepatitis E virus-like particles in Hecolin®

Vaccine ◽  
2014 ◽  
Vol 32 (32) ◽  
pp. 4039-4050 ◽  
Author(s):  
Xiao Zhang ◽  
Minxi Wei ◽  
Huirong Pan ◽  
Zhijie Lin ◽  
Kaihang Wang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
Virus Like Particles ◽  
Robust Manufacturing ◽  
Comprehensive Characterization

Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles

2017 ◽  
Vol 102 (1) ◽  
pp. 185-198 ◽  
Author(s):  
Martynas Simanavicius ◽  
Paulius Lukas Tamosiunas ◽  
Rasa Petraityte-Burneikiene ◽  
Reimar Johne ◽  
Rainer G. Ulrich ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Capsid ◽  
Virus Like Particles ◽  
Antigenic Characterization ◽  
Virus Capsid Protein

Expression and characterization of hepatitis E virus-like particles and non-virus-like particles from insect cells

2015 ◽  
Vol 63 (3) ◽  
pp. 362-370 ◽  
Author(s):  
Ying Qi ◽  
Jinping Fan ◽  
Weijin Huang ◽  
Chenyan Zhao ◽  
Youchun Wang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Insect Cells ◽  
Hepatitis E ◽  
Virus Like Particles

scholarly journals Antigenic Characterization of ORF2 and ORF3 Proteins of Hepatitis E Virus (HEV)

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1385
Author(s):  
Giulia Pezzoni ◽  
Lidia Stercoli ◽  
Eleonora Pegoiani ◽  
Emiliana Brocchi
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Antigen ◽  
Open Reading Frame ◽  
Reading Frame ◽  
E Coli ◽  
Antigenic Characterization ◽  
Antigenic Properties ◽  
Open Reading Frame 2

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.

scholarly journals Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Gergana Zahmanova ◽  
Milena Mazalovska ◽  
Katerina Takova ◽  
Valentina Toneva ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nicotiana Benthamiana ◽  
Hepatitis E Virus ◽  
Chimeric Protein ◽  
Hepatitis E ◽  
Core Antigen ◽  
Efficient Production ◽  
Virus Like Particles ◽  
B Virus

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

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