scholarly journals The Skin Microbiota of Eleutherodactylus Frogs: Effects of Host Ecology, Phylogeny, and Local Environment

2019 ◽  
Vol 10 ◽  
Author(s):  
Liza Garcia-Recinos ◽  
Patricia A. Burrowes ◽  
Maria Dominguez-Bello
Keyword(s):  
Local Environment ◽  
Skin Microbiota ◽  
Host Ecology

scholarly journals Ecology and Host Identity Outweigh Evolutionary History in Shaping the Bat Microbiome

mSystems ◽  
2019 ◽  
Vol 4 (6) ◽  
Author(s):  
Holly L. Lutz ◽  
Elliot W. Jackson ◽  
Paul W. Webala ◽  
Waswa S. Babyesiza ◽  
Julian C. Kerbis Peterhans ◽  
...  
Keyword(s):  
Microbial Community ◽  
Bacterial Community ◽  
Community Composition ◽  
Evolutionary History ◽  
Bacterial Community Composition ◽  
Microbial Community Composition ◽  
Anatomical Site ◽  
Skin Microbiota ◽  
Host Identity ◽  
Host Ecology

ABSTRACT Recent studies of mammalian microbiomes have identified strong phylogenetic effects on bacterial community composition. Bats (Mammalia: Chiroptera) are among the most speciose mammals on the planet and the only mammal capable of true flight. We examined 1,236 16S rRNA amplicon libraries of the gut, oral, and skin microbiota from 497 Afrotropical bats (representing 9 families, 20 genera, and 31 species) to assess the extent to which host ecology and phylogeny predict microbial community similarity in bats. In contrast to recent studies of host-microbe associations in other mammals, we found no correlation between chiropteran phylogeny and bacterial community dissimilarity across the three anatomical sites sampled. For all anatomical sites, we found host species identity and geographic locality to be strong predictors of microbial community composition and observed a positive correlation between elevation and bacterial richness. Last, we identified significantly different bacterial associations within the gut microbiota of insectivorous and frugivorous bats. We conclude that the gut, oral, and skin microbiota of bats are shaped predominantly by ecological factors and do not exhibit the same degree of phylosymbiosis observed in other mammals. IMPORTANCE This study is the first to provide a comprehensive survey of bacterial symbionts from multiple anatomical sites across a broad taxonomic range of Afrotropical bats, demonstrating significant associations between the bat microbiome and anatomical site, geographic locality, and host identity—but not evolutionary history. This study provides a framework for future systems biology approaches to examine host-symbiont relationships across broad taxonomic scales, emphasizing the need to elucidate the interplay between host ecology and evolutionary history in shaping the microbiome of different anatomical sites.

scholarly journals Skin microbiota differs drastically between co-occurring frogs and newts

2017 ◽  
Vol 4 (4) ◽  
pp. 170107 ◽  
Author(s):  
Molly C. Bletz ◽  
R. G. Bina Perl ◽  
Miguel Vences
Keyword(s):  
Bacterial Communities ◽  
Operational Taxonomic Unit ◽  
Amplicon Sequencing ◽  
Amphibian Skin ◽  
Skin Microbiota ◽  
Triturus Cristatus ◽  
Bacterial Composition ◽  
Host Ecology ◽  
Rana Dalmatina ◽  
Ichthyosaura Alpestris

Diverse microbial assemblages inhabit amphibian skin and are known to differ among species; however, few studies have analysed these differences in systems that minimize confounding factors, such as season, location or host ecology. We used high-throughput amplicon sequencing to compare cutaneous microbiotas among two ranid frogs ( Rana dalmatina, R. temporaria ) and four salamandrid newts ( Ichthyosaura alpestris, Lissotriton helveticus, L. vulgaris, Triturus cristatus ) breeding simultaneously in two ponds near Braunschweig, Germany. We found that bacterial communities differed strongly and consistently between these two distinct amphibian clades. While frogs and newts had similar cutaneous bacterial richness, their bacterial composition strongly differed. Average Jaccard distances between frogs and newts were over 0.5, while between species within these groups distances were only 0.387 and 0.407 for frogs and newts, respectively. At the operational taxonomic unit (OTU) level, 31 taxa exhibited significantly different relative abundances between frogs and newts. This finding suggests that chemical or physical characteristics of these amphibians' mucosal environments provide highly selective conditions for bacterial colonizers. Multi-omics analyses of hosts and their microbiota as well as directed efforts to understand chemical differences in the mucosal environments (e.g. pH), and the specificities of host-produced compounds against potential colonizers will help to better understand this intriguing pattern.

Residual Gas Reaction in the Electron Microscope: II The Design of a Gas-Control Chamber for Quantitative Observations

1969 ◽  
Vol 27 ◽  
pp. 82-83
Author(s):  
Chester J. Calbick ◽  
Richard E. Hartman
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Local Environment ◽  
Chamber Pressure ◽  
Objective Lens ◽  
Ion Pump ◽  
Quantitative Studies ◽  
Precise Knowledge ◽  
Differential Pumping ◽  
And Control

Quantitative studies of the phenomenon associated with reactions induced by the electron beam between specimens and gases present in the electron microscope require precise knowledge and control of the local environment experienced by the portion of the specimen in the electron beam. Because of outgassing phenomena, the environment at the irradiated portion of the specimen is very different from that in any place where gas pressures and compositions can be measured. We have found that differential pumping of the specimen chamber by a 4" Orb-Ion pump, following roughing by a zeolite sorption pump, can produce a specimen-chamber pressure 100- to 1000-fold less than that in the region below the objective lens.

An emerging technology: Nuclear magnetic resonance microscopy

1988 ◽  
Vol 46 ◽  
pp. 1000-1001
Author(s):  
M.J. Hennessy ◽  
E. Kwok
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Relaxation Times ◽  
Basic Research ◽  
Local Environment ◽  
Magnetic Resonance Images ◽  
Nmr Microscopy ◽  
Mri Scans ◽  
Temperature Relaxation ◽  
Nuclear Magnetic

Much progress in nuclear magnetic resonance microscope has been made in the last few years as a result of improved instrumentation and techniques being made available through basic research in magnetic resonance imaging (MRI) technologies for medicine. Nuclear magnetic resonance (NMR) was first observed in the hydrogen nucleus in water by Bloch, Purcell and Pound over 40 years ago. Today, in medicine, virtually all commercial MRI scans are made of water bound in tissue. This is also true for NMR microscopy, which has focussed mainly on biological applications. The reason water is the favored molecule for NMR is because water is,the most abundant molecule in biology. It is also the most NMR sensitive having the largest nuclear magnetic moment and having reasonable room temperature relaxation times (from 10 ms to 3 sec). The contrast seen in magnetic resonance images is due mostly to distribution of water relaxation times in sample which are extremely sensitive to the local environment.

Electron energy-loss near-edge structure in silicate minerals

1992 ◽  
Vol 50 (2) ◽  
pp. 1258-1259
Author(s):  
D W McComb ◽  
R S Payne ◽  
P L Hansen ◽  
R Brydson
Keyword(s):  
Solid State ◽  
Energy Loss ◽  
Electron Energy ◽  
State Energy ◽  
Local Environment ◽  
Edge Structure ◽  
Silicate Minerals ◽  
Two Samples ◽  
Electron Energy Loss ◽  
Careful Processing

Electron energy-loss near-edge structure (ELNES) is an effective probe of the local geometrical and electronic environment around particular atomic species in the solid state. Energy-loss spectra from several silicate minerals were mostly acquired using a VG HB501 STEM fitted with a parallel detector. Typically a collection angle of ≈8mrad was used, and an energy resolution of ≈0.5eV was achieved.Other authors have indicated that the ELNES of the Si L2,3-edge in α-quartz is dominated by the local environment of the silicon atom i.e. the SiO4 tetrahedron. On this basis, and from results on other minerals, the concept of a coordination fingerprint for certain atoms in minerals has been proposed. The concept is useful in some cases, illustrated here using results from a study of the Al2SiO5 polymorphs (Fig.l). The Al L2,3-edge of kyanite, which contains only 6-coordinate Al, is easily distinguished from andalusite (5- & 6-coordinate Al) and sillimanite (4- & 6-coordinate Al). At the Al K-edge even the latter two samples exhibit differences; with careful processing, the fingerprint for 4-, 5- and 6-coordinate aluminium may be obtained.

ALKYL CH STRETCH VIBRATIONS AS A PROBE OF LOCAL ENVIRONMENT AND STRUCTURE

2015 ◽  
Author(s):  
Edwin Sibert ◽  
Timothy Zwier ◽  
Jacob Dean ◽  
Nathanael Kidwell ◽  
Daniel Tabor
Keyword(s):  
Local Environment

Motivations and attitudes toward domestic tourism in Saudi Arabia

2019 ◽  
Vol 9 (1) ◽  
pp. 27-37
Author(s):  
Mourad Mansour ◽  
Alhassan G Mumuni
Keyword(s):  
Saudi Arabia ◽  
Local Environment ◽  
Domestic Environment ◽  
Domestic Tourism ◽  
Tourism Destinations ◽  
Tourism Information ◽  
The Government ◽  
Using Data ◽  
Tourism Organization ◽  
Self Administered Questionnaire

AbstractBeginning with the establishment of a Supreme Commission for Tourism and Antiquities’ (SCTA) in 2000, there have been official attempts by the government of Saudi Arabia to encourage domestic tourism in order to tap into the huge amounts that Saudis spend annually on vacations. This paper examines the motivations and attitudes of consumers toward tourism destinations and activities within the country (domestic tourism). Using data collected through a structured self-administered questionnaire, the study finds that familiarity and trust of the local environment, perceptions of the safer domestic environment, and limitations imposed by respondents’ vacation timing are the primary motives for choosing to spend their vacations locally, while lack of quality domestic tourist sites and services (including entertainment facilities), lack of tourism information, insufficient tourism organization services, and the harsh local environmental conditions during summer are factors that ‘push’ people from spending the vacations locally. Attitudes toward domestic tourism are generally negative, although there are significant differences in attitudes between respondents who prefer domestic destinations and those who prefer to travel out of Kingdom. Implications of the findings are outlined and discussed.

Local environment surrounding the MSX satellite during the first week after launch

1997 ◽  
Author(s):  
B. Green ◽  
Gary Galica ◽  
Phillip Mulhall ◽  
O. Uy ◽  
Jeffrey Lesho ◽  
...  
Keyword(s):  
Local Environment
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