scholarly journals Carbapenem-Resistant Acinetobacter baumannii in Three Tertiary Care Hospitals in Mexico: Virulence Profiles, Innate Immune Response and Clonal Dissemination

2019 ◽  
Vol 10 ◽  
Author(s):  
María Dolores Alcántar-Curiel ◽  
Roberto Rosales-Reyes ◽  
Ma Dolores Jarillo-Quijada ◽  
Catalina Gayosso-Vázquez ◽  
José Luis Fernández-Vázquez ◽  
...  
Keyword(s):  
Immune Response ◽  
Acinetobacter Baumannii ◽  
Innate Immune Response ◽  
Tertiary Care ◽  
Innate Immune ◽  
Carbapenem Resistant ◽  
Tertiary Care Hospitals

scholarly journals Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii

2019 ◽  
Vol 13 (10) ◽  
pp. 899-905 ◽  
Author(s):  
Mohsin Khurshid ◽  
Muhammad Hidayat Rasool ◽  
Muhammad Hussnain Siddique ◽  
Farrukh Azeem ◽  
Muhammad Naeem ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Molecular Mechanisms ◽  
Antimicrobial Agents ◽  
Tertiary Care ◽  
Antibiotic Resistance Genes ◽  
Polymyxin B ◽  
Beta Lactam ◽  
Genetic Determinants ◽  
Carbapenem Resistant ◽  
Tertiary Care Hospitals

Introduction: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of aminoglycosides, tetracycline, and sulfonamide-resistant determinants among CRAB isolates that carry the blaOXA-23 gene. Methodology: For a total of 160 CRAB strains isolated at tertiary care hospitals of Pakistan that mainly carried blaOXA-23 gene were included in the study to evaluate the assortment of antibiotic resistance genes. Results: The susceptibility rates of CRAB for other than beta-lactam drugs were 2.5% for both ciprofloxacin and aminoglycosides and 18% and 25% for sulfonamides and tetracyclines, respectively. Polymyxin B (MIC90, 1 g/mL) Colistin (MIC90, 1 g/mL) and Tigecycline (MIC90, 2 g/mL) were most active against these extensively drug-resistant CRAB isolates. The isolates were found to possess various genes mainly the tetB and sul2 for tetracycline and sulfonamide but the genes conferring resistance to aminoglycosides were varied with various combinations. Conclusion: Despite the CRAB clones containing blaOXA-23 have been previously reported in Pakistani hospitals, the screening of genetic determinants responsible for other antimicrobial agents is crucial for developing an effective surveillance and mitigation system for infection management.

scholarly journals Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71751 ◽  
Author(s):  
So Hyun Jun ◽  
Jung Hwa Lee ◽  
Bo Ra Kim ◽  
Seung Il Kim ◽  
Tae In Park ◽  
...  
Keyword(s):  
Immune Response ◽  
Membrane Proteins ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Innate Immune Response ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Innate Immune

scholarly journals Dissemination of Acinetobacter baumannii Clones with OXA-23 Carbapenemase in Colombian Hospitals

2007 ◽  
Vol 51 (6) ◽  
pp. 2001-2004 ◽  
Author(s):  
Maria Virginia Villegas ◽  
Juan Nicolas Kattan ◽  
Adriana Correa ◽  
Karen Lolans ◽  
Ana Maria Guzman ◽  
...  
Keyword(s):  
South America ◽  
Acinetobacter Baumannii ◽  
Tertiary Care ◽  
Multidrug Resistant ◽  
Surveillance Network ◽  
Carbapenem Resistant ◽  
Nationwide Surveillance ◽  
Tertiary Care Hospitals ◽  
Widespread Dissemination

ABSTRACT During 2005, 66 carbapenem-resistant isolates of Acinetobacter baumannii were collected from seven tertiary-care hospitals participating in a nationwide surveillance network in Colombia. The isolates were multidrug resistant and produced the carbapenemases OXA-23 and OXA-51. Forty-five belonged to four clones while 21 were unique pulsotypes. One clone was present in two hospitals within one city, while another had spread between two hospitals in different cities. Blood, secretions, and abdominal fluids were the most frequent sites of isolation. This is the first description of widespread dissemination of OXA-23 in South America.

scholarly journals Epithelial Innate Immune Response to Acinetobacter baumannii Challenge

2014 ◽  
Vol 82 (11) ◽  
pp. 4458-4465 ◽  
Author(s):  
Zhimin Feng ◽  
Xun Jia ◽  
Mark D. Adams ◽  
Santosh K. Ghosh ◽  
Robert A. Bonomo ◽  
...  
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Acinetobacter Baumannii ◽  
Innate Immune Response ◽  
Care Delivery ◽  
Growth Factor Receptor ◽  
Adaptive Immune System ◽  
Innate Immune ◽  
Mitogen Activated Protein Kinase ◽  
Content Type

ABSTRACTCurrently,Acinetobacter baumanniiis recognized as one of the major pathogens seriously threatening our health care delivery system. Aspects of the innate immune response toA. baumanniiinfection are not yet well understood. Human β-defensins (hBDs) are epithelial cell-derived cationic antimicrobial peptides (AMPs) that also function to bridge the innate and adaptive immune system. We tested the induction of hBD-2 and -3 byA. baumanniion primary oral and skin epithelial cells and found thatA. baumanniiinduces hBD-3 transcripts to a greater extent than it induces hBD-2 transcripts on both types of cells. In addition, we found thatA. baumanniiis susceptible to hBD-2 and -3 killing at submicromolar concentrations. Moreover, hBD-3 induction byA. baumanniiwas found to be dependent on epidermal growth factor receptor (EGFR) signaling. Inhibition of mitogen-activated protein kinase resulted in reduced expression of both hBD-2 and -3. Lastly, a disintegrin and metalloprotease 17 (ADAM17; also known as TACE) was found to be critical for hBD-3 induction, while ADAM10 and dual oxidase 1 (Duox1) were not required for hBD-3 induction. Induction of AMPs is an important component of innate sensing of pathogens and may play an important role in triggering systemic immune responses toA. baumanniiinfection. Further studies on the interactions between epithelial cells andA. baumanniiwill help us understand early stages of infection and may shed light on why some individuals are more vulnerable toA. baumanniiinfection.

scholarly journals Prevalence of OXA-Type β-Lactamase Genes among Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Thailand

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 864
Author(s):  
Krit Thirapanmethee ◽  
Thayapa Srisiri-a-nun ◽  
Jantana Houngsaitong ◽  
Preecha Montakantikul ◽  
Piyatip Khuntayaporn ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Resistance Mechanisms ◽  
Clinical Isolates ◽  
Health Concern ◽  
Drug Of Choice ◽  
Carbapenem Resistant ◽  
Resistance Rates ◽  
Tertiary Care Hospitals

Carbapenem-resistant Acinetobacter baumannii (CRAB) is a critical health concern for the treatment of infectious diseases. The aim of this study was to investigate the molecular epidemiology of CRAB emphasizing the presence of oxacillinase (OXA)-type β-lactamase-encoding genes, one of the most important carbapenem resistance mechanisms. In this study, a total of 183 non-repetitive CRAB isolates collected from 11 tertiary care hospitals across Thailand were investigated. As a result, the blaoxa-51-like gene, an intrinsic enzyme marker, was detected in all clinical isolates. The blaoxa-23-like gene was presented in the majority of isolates (68.31%). In contrast, the prevalence rates of blaoxa-40/24-like and blaoxa-58-like gene occurrences in CRAB isolates were only 4.92% and 1.09%, respectively. All isolates were resistant to carbapenems, with 100% resistance to imipenem, followed by meropenem (98.91%) and doripenem (94.54%). Most isolates showed high resistance rates to ciprofloxacin (97.81%), ceftazidime (96.72%), gentamicin (91.26%), and amikacin (80.87%). Interestingly, colistin was found to be a potential drug of choice due to the high susceptibility of the tested isolates to this antimicrobial (87.98%). Most CRAB isolates in Thailand were of ST2 lineage, but some belonged to ST25, ST98, ST129, ST164, ST215, ST338, and ST745. Further studies to monitor the spread of carbapenem-resistant OXA-type β-lactamase genes from A. baumannii in hospital settings are warranted.

scholarly journals Differences in Acinetobacter baumannii Strains and Host Innate Immune Response Determine Morbidity and Mortality in Experimental Pneumonia

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e30673 ◽  
Author(s):  
Anna de Breij ◽  
Matthieu Eveillard ◽  
Lenie Dijkshoorn ◽  
Peterhans J. van den Broek ◽  
Peter H. Nibbering ◽  
...  
Keyword(s):  
Immune Response ◽  
Acinetobacter Baumannii ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Morbidity And Mortality ◽  
Experimental Pneumonia ◽  
Host Innate Immune Response

Oral Immune Activation by Disgust and Disease-Related Pictures

2015 ◽  
Vol 29 (3) ◽  
pp. 119-129 ◽  
Author(s):  
Richard J. Stevenson ◽  
Deborah Hodgson ◽  
Megan J. Oaten ◽  
Luba Sominsky ◽  
Mehmet Mahmut ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Innate Immune Response ◽  
Negative Control ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Immune Markers ◽  
Before And After ◽  
Secondary Analyses ◽  
Image Set

Abstract. Both disgust and disease-related images appear able to induce an innate immune response but it is unclear whether these effects are independent or rely upon a common shared factor (e.g., disgust or disease-related cognitions). In this study we directly compared these two inductions using specifically generated sets of images. One set was disease-related but evoked little disgust, while the other set was disgust evoking but with less disease-relatedness. These two image sets were then compared to a third set, a negative control condition. Using a wholly within-subject design, participants viewed one image set per week, and provided saliva samples, before and after each viewing occasion, which were later analyzed for innate immune markers. We found that both the disease related and disgust images, relative to the negative control images, were not able to generate an innate immune response. However, secondary analyses revealed innate immune responses in participants with greater propensity to feel disgust following exposure to disease-related and disgusting images. These findings suggest that disgust images relatively free of disease-related themes, and disease-related images relatively free of disgust may be suboptimal cues for generating an innate immune response. Not only may this explain why disgust propensity mediates these effects, it may also imply a common pathway.

IL-17C is a mediator of respiratory epithelial innate immune response

Pneumologie ◽  
2013 ◽  
Vol 67 (S 01) ◽  
Author(s):  
P Pfeifer ◽  
M Voss ◽  
B Wonnenberg ◽  
M Bischoff ◽  
F Langer ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Respiratory Epithelial
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