scholarly journals Sequence Determinants Spanning −10 Motif and Spacer Region Implicated in Unique Ehrlichia chaffeensis Sigma 32-Dependent Promoter Activity of dnaK Gene

2019 ◽  
Vol 10 ◽  
Author(s):  
Huitao Liu ◽  
Roman R. Ganta
Keyword(s):  
Promoter Activity ◽  
Ehrlichia Chaffeensis ◽  
Sigma 32

scholarly journals Transcription of Ehrlichia chaffeensis Genes Is Accomplished by RNA Polymerase Holoenzyme Containing either Sigma 32 or Sigma 70

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e81780 ◽  
Author(s):  
Huitao Liu ◽  
Tonia Von Ohlen ◽  
Chuanmin Cheng ◽  
Bonto Faburay ◽  
Roman R. Ganta
Keyword(s):  
Rna Polymerase ◽  
Ehrlichia Chaffeensis ◽  
Sigma 70 ◽  
Rna Polymerase Holoenzyme ◽  
Sigma 32

scholarly journals Sigma 32-Dependent Promoter Activity In Vivo: Sequence Determinants of the groE Promoter

2003 ◽  
Vol 185 (19) ◽  
pp. 5800-5806 ◽  
Author(s):  
Yang Wang ◽  
Pieter L. deHaseth
Keyword(s):  
Escherichia Coli ◽  
Transcription Factor ◽  
Transcription Initiation ◽  
Promoter Activity ◽  
Rna Synthesis ◽  
The Other ◽  
Base Pairs ◽  
E Coli ◽  
Sigma 32

ABSTRACT The Escherichia coli transcription factor sigma 32 binds to core RNA polymerase to form the holoenzyme responsible for transcription initiation at heat shock promoters, utilized upon exposure of the cell to higher temperatures. We have developed two ways to assay sigma 32-dependent RNA synthesis in E. coli. The plasmid-borne reporter gene for both is lacZ (β-galactosidase), driven by the groE promoter. In one application, the cells are exposed to a temperature of 42°C in order to induce accumulation of endogenous sigma 32. The other involves isopropylthiogalactopyranoside (IPTG)-induced synthesis of sigma 32 at 30°C from a gene contained on a second plasmid. The latter employs DnaK− cells, which additionally contained a second mutation, inactivating the endogenous sigma 32 gene (Bukau and Walker, EMBO J. 9:4027-4036, 1990). These assays were used to delineate the sequences CTTGA (−37 to −33) and GNCCCCATNT (−18 to −9) as important for sigma 32 promoter activity. At each of the specified base pairs, substitutions were found which reduced promoter activity by greater than 75%. Activity was also dependent upon the number of base pairs separating the two regions.

Exemplar Abstract for Ehrlichia chaffeensis Anderson et al. 1992 emend. Hördt et al. 2020.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Ehrlichia Chaffeensis

UBE2L6 is Involved in Cisplatin Resistance by Regulating the Transcription of ABCB6

2020 ◽  
Vol 20 (12) ◽  
pp. 1487-1496 ◽  
Author(s):  
Midori Murakami ◽  
Hiroto Izumi ◽  
Tomoko Kurita ◽  
Chiho Koi ◽  
Yasuo Morimoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Promoter Activity ◽  
Anticancer Agent ◽  
Cisplatin Resistance ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Molecular Target ◽  
Transcriptional Level ◽  
And Control ◽  
Resistant Cells

Background: Cisplatin is an important anticancer agent in cancer chemotherapy, but when resistant cells appear, treatment becomes difficult, and the prognosis is poor. Objective: In this study, we investigated the gene expression profile in cisplatin sensitive and resistant cells, and identified the genes involved in cisplatin resistance. Methods: Comparison of gene expression profiles revealed that UBE2L6 mRNA is highly expressed in resistant cells. To elucidate whether UBE2L6 is involved in the acquisition of cisplatin resistance, UBE2L6- overexpressing cells established from cisplatin-sensitive cells and UBE2L6-silenced cells developed from cisplatin- resistant cells were generated, and the sensitivity of cisplatin was examined. Results: The sensitivity of the UBE2L6-overexpressing cells did not change compared with the control cells, but the UBE2L6-silenced cells were sensitized to cisplatin. To elucidate the mechanism of UBE2L6 in cisplatin resistance, we compared the gene expression profiles of UBE2L6-silenced cells and control cells and found that the level of ABCB6 mRNA involved in cisplatin resistance was decreased. Moreover, ABCB6 promoter activity was partially suppressed in UBE2L6-silenced cells. Conclusion: These results suggest that cisplatin-resistant cells have upregulated UBE2L6 expression and contribute to cisplatin resistance by regulating ABCB6 expression at the transcriptional level. UBE2L6 might be a molecular target that overcomes cisplatin resistance.

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