scholarly journals Laboratory Evolution Assays and Whole-Genome Sequencing for the Development and Safety Evaluation of Lactobacillus plantarum With Stable Resistance to Gentamicin

2019 ◽  
Vol 10 ◽  
Author(s):  
Yushan Dong ◽  
Fuxin Zhang ◽  
Bini Wang ◽  
Jiayuan Gao ◽  
Jiangtao Zhang ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Lactobacillus Plantarum ◽  
Genome Sequencing ◽  
Safety Evaluation ◽  
Whole Genome ◽  
Laboratory Evolution

scholarly journals Whole-genome sequencing reveals the mechanisms for evolution of streptomycin resistance in Lactobacillus plantarum

2018 ◽  
Vol 101 (4) ◽  
pp. 2867-2874 ◽  
Author(s):  
Fuxin Zhang ◽  
Jiayuan Gao ◽  
Bini Wang ◽  
Dongxue Huo ◽  
Zhaoxia Wang ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Lactobacillus Plantarum ◽  
Genome Sequencing ◽  
Streptomycin Resistance ◽  
Whole Genome

scholarly journals In vivorecombination ofSaccharomyces eubayanusmaltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation

2018 ◽  
Author(s):  
Nick Brouwers ◽  
Arthur R. Gorter de Vries ◽  
Marcel van den Broek ◽  
Susan M. Weening ◽  
Tom D. Elink Schuurman ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Tertiary Structure ◽  
Mosaic Structure ◽  
Whole Genome ◽  
Transporter Gene ◽  
Laboratory Evolution ◽  
Saccharomyces Pastorianus ◽  
Maltose Transporter ◽  
Flavor Profile

AbstractSaccharomyces pastorianuslager-brewing yeasts are aneuploidS. cerevisiaexS. eubayanushybrids, whose genomes have been shaped by domestication in brewing-related contexts. In contrast to mostS. cerevisiaeandS. pastorianusstrains,S. eubayanuscannot utilize maltotriose, a major carbohydrate in brewer’s wort. Accordingly,S. eubayanusCBS 12357⊤harbors fourSeMALTmaltose-transporter genes, but no genes resembling theS. cerevisiaemaltotriose-transporter geneScAGT1or theS. pastorianusmaltotriose-transporter geneSpMTY1. To study the evolvability of maltotriose utilization inS. eubayanusCBS 12357⊤, maltotriose-assimilating mutants obtained after UV mutagenesis were subjected to laboratory evolution in carbon-limited chemostat cultures on maltotriose-enriched wort. An evolved strain showed improved maltose and maltotriose fermentation, as well as an improved flavor profile, in 7-L fermenter experiments on industrial wort. Whole-genome sequencing revealed a novel mosaicSeMALT413gene, resulting from repeated gene introgressions by non-reciprocal translocation of at least threeSeMALTgenes. The predicted tertiary structure of SeMalt413 was comparable to the original SeMalt transporters, but overexpression ofSeMALT413sufficed to enable growth on maltotriose, indicating gene neofunctionalization had occurred. The mosaic structure ofSeMALT413resembles the structure ofS. pastorianusmaltotriose-transporter geneSpMTY1, which has sequences with high similarity to alternatinglyScMALx1andSeMALT3. Evolution of the maltotriose-transporter landscape in hybridS. pastorianuslager-brewing strains is therefore likely to have involved mechanisms similar to those observed in the present study.Author SummaryFermentation of the wort sugar maltotriose is critical for the flavor profile obtained during beer brewing. The recently discovered yeastSaccharomyces eubayanusis gaining popularity as an alternative toS. pastorianusandS. cerevisiaefor brewing, however it is unable to utilize maltotriose. Here, a combination of non-GMO mutagenesis and laboratory evolution of theS. eubayanustype strain CBS 12357⊤was used to enable maltotriose fermentation in brewer’s wort. A resultingS. eubayanusstrain showed a significantly improved brewing performance, including improved maltose and maltotriose consumption and a superior flavor profile. Whole genome sequencing identified a novel transporter gene,SeMALT413, which was formed by recombination between three differentSeMALTmaltose-transporter genes. Overexpression ofSeMALT413in CBS 12357⊤confirmed its neofunctionalization as a maltotriose transporter. The mosaic structure of the maltotriose transporter SpMty1 inS. pastorianusresembles that of SeMalt413, suggesting that maltotriose utilization likely emerged through similar recombination events during the domestication of current lager brewing strains.

Whole genome sequencing of Lactobacillus plantarum DMDL 9010 and its effect on growth phenotype under nitrite stress

LWT ◽  
2021 ◽  
pp. 111778
Author(s):  
Yan-yan Huang ◽  
Dong-mei Liu ◽  
Xiang-ze Jia ◽  
Ming-hua Liang ◽  
Yongzhi Lu ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Lactobacillus Plantarum ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Growth Phenotype ◽  
Effect On Growth

Utility of Whole Genome Sequencing in diagnosing complex disorders: lesson from renal tubular disorders

2018 ◽  
Author(s):  
Mark Stevenson ◽  
Alistair T Pagnamenta ◽  
Heather G Mack ◽  
Judith A Savige ◽  
Kate E Lines ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Complex Disorders ◽  
Tubular Disorders ◽  
Renal Tubular Disorders ◽  
Renal Tubular

0306 Exploring the feasibility of using copy number variants as genetic markers through large-scale whole genome sequencing experiments

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 146-146
Author(s):  
D. M. Bickhart ◽  
L. Xu ◽  
J. L. Hutchison ◽  
J. B. Cole ◽  
D. J. Null ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genetic Markers ◽  
Genome Sequencing ◽  
Copy Number ◽  
Large Scale ◽  
Copy Number Variants ◽  
Whole Genome
Sign in / Sign up

Export Citation Format

Share Document