scholarly journals Bacteroides thetaiotaomicron Starch Utilization Promotes Quercetin Degradation and Butyrate Production by Eubacterium ramulus

2019 ◽  
Vol 10 ◽  
Author(s):  
Gina Paola Rodriguez-Castaño ◽  
Matthew R. Dorris ◽  
Xingbo Liu ◽  
Bradley W. Bolling ◽  
Alejandro Acosta-Gonzalez ◽  
...  
Keyword(s):  
Bacteroides Thetaiotaomicron ◽  
Starch Utilization ◽  
Butyrate Production

scholarly journals Bacteroides thetaiotaomicron Fosters the Growth of Butyrate-Producing Anaerostipes caccae in the Presence of Lactose and Total Human Milk Carbohydrates

2020 ◽  
Vol 8 (10) ◽  
pp. 1513
Author(s):  
Loo Wee Chia ◽  
Marko Mank ◽  
Bernadet Blijenberg ◽  
Steven Aalvink ◽  
Roger S. Bongers ◽  
...  
Keyword(s):  
Human Milk ◽  
Early Life ◽  
Low Molecular Weight ◽  
Proof Of Concept ◽  
Human Milk Oligosaccharides ◽  
Bacteroides Thetaiotaomicron ◽  
Microbial Network ◽  
Anaerobic Bioreactors ◽  
Set Up ◽  
Butyrate Production

The development of infant gut microbiota is strongly influenced by nutrition. Human milk oligosaccharides (HMOSs) in breast milk selectively promote the growth of glycan-degrading microbes, which lays the basis of the microbial network. In this study, we investigated the trophic interaction between Bacteroides thetaiotaomicron and the butyrate-producing Anaerostipes caccae in the presence of early-life carbohydrates. Anaerobic bioreactors were set up to study the monocultures of B. thetaiotaomicron and the co-cultures of B. thetaiotaomicron with A. caccae in minimal media supplemented with lactose or a total human milk carbohydrate fraction. Bacterial growth (qPCR), metabolites (HPLC), and HMOS utilization (LC-ESI-MS2) were monitored. B. thetaiotaomicron displayed potent glycan catabolic capability with differential preference in degrading specific low molecular weight HMOSs, including the neutral trioses (2′-FL and 3-FL), neutral tetraoses (DFL, LNT, LNnT), neutral pentaoses (LNFP I, II, III, V), and acidic trioses (3′-SL and 6′-SL). In contrast, A. caccae was not able to utilize lactose and HMOSs. However, the signature metabolite of A. caccae, butyrate, was detected in co-culture with B. thetaiotaomicron. As such, A. caccae cross-fed on B. thetaiotaomicron-derived monosaccharides, acetate, and d-lactate for growth and concomitant butyrate production. This study provides a proof of concept that B. thetaiotaomicron could drive the butyrogenic metabolic network in the infant gut.

Suggested alternative starch utilization system from the human gut bacterium Bacteroides thetaiotaomicron

2016 ◽  
Vol 94 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Marcia M. Chaudet ◽  
David R. Rose
Keyword(s):  
Outer Membrane ◽  
Bacterial Species ◽  
Protein A ◽  
Human Colon ◽  
Bacteroides Thetaiotaomicron ◽  
Starch Utilization ◽  
Human Digestive System ◽  
Polysaccharide Utilization Loci

The human digestive system is host to a highly populated ecosystem of bacterial species that significantly contributes to our assimilation of dietary carbohydrates. Bacteroides thetaiotaomicron is a member of this ecosystem, and participates largely in the role of the gut microbiome by breaking down dietary complex carbohydrates. This process of acquiring glycans from the colon lumen is predicted to rely on the mechanisms of proteins that are part of a classified system known as polysaccharide utilization loci (PUL). These loci are responsible for binding substrates at the cell outer membrane, internalizing them, and then hydrolyzing them within the periplasm into simple sugars. Here we report our investigation into specific components of a PUL, and suggest an alternative starch utilization system in B. thetaiotaomicron. Our analysis of an outer membrane binding protein, a SusD homolog, highlights its contribution to this PUL by acquiring starch-based sugars from the colon lumen. Through our structural characterization of two Family GH31 α-glucosidases, we reveal the flexibility of this bacterium with respect to utilizing a range of starch-derived glycans with an emphasis on branched substrates. With these results we demonstrate the predicted function of a gene locus that is capable of contributing to starch hydrolysis in the human colon.

scholarly journals Utilization of four galactans by Bacteroides thetaiotaomicron A4 based on transcriptome

2021 ◽  
Author(s):  
Song Li ◽  
Baojie Zhang ◽  
Jielun Hu ◽  
Yadong Zhong ◽  
Yonggan Sun ◽  
...  
Keyword(s):  
Bacteroides Thetaiotaomicron
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