scholarly journals Mevalonate Diphosphate Decarboxylase MVD/Erg19 Is Required for Ergosterol Biosynthesis, Growth, Sporulation and Stress Tolerance in Aspergillus oryzae

2019 ◽  
Vol 10 ◽  
Author(s):  
Yunlong Sun ◽  
Yali Niu ◽  
Hui Huang ◽  
Bin He ◽  
Long Ma ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Aspergillus Oryzae ◽  
Ergosterol Biosynthesis ◽  
Mevalonate Diphosphate Decarboxylase

scholarly journals Effects on Gene Transcription Profile and Fatty Acid Composition by Genetic Modification of Mevalonate Diphosphate Decarboxylase MVD/Erg19 in Aspergillus Oryzae

2019 ◽  
Vol 7 (9) ◽  
pp. 342
Author(s):  
Hu ◽  
Huang ◽  
Sun ◽  
Niu ◽  
Xu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gene Transcription ◽  
Aspergillus Oryzae ◽  
Acid Content ◽  
Ergosterol Biosynthesis ◽  
Transcription Profile ◽  
Control Strain ◽  
Overexpression Strain ◽  
Mevalonate Diphosphate Decarboxylase ◽  
Gene Transcription Profile

Mevalonate diphosphate decarboxylase MVD/Erg19 is required for ergosterol biosynthesis, growth, sporulation, and stress tolerance in Aspergillus oryzae. In this study, RNA-seq was used to analyze the gene transcription profile in AoErg19 overexpression (OE) and RNAi strains. There were 256 and 74 differentially expressed genes (DEGs) in AoErg19 OE and RNAi strains, respectively, compared with the control strain (CK). The most common DEGs were transport- and metabolism-related genes. Only 22 DEGs were obtained that were regulated in both OE and RNAi strains. The transcriptomic comparison between CK and AoErg19 overexpression strain (CK vs. OE), and between CK and AoErg19 RNAi strain (CK vs. RNAi) revealed that the greatest difference existed in the number of genes belonging to the cytochrome P450 family; 12 were found in CK vs. OE, whereas 1 was found in CK vs. RNAi. The expression patterns of lipid biosynthesis and metabolism related genes were altered in OE and RNAi strains, either by gene induction or suppression. Moreover, the total fatty acid content in the RNAi strain was 12.1% greater than the control strain, but no difference in total acid content was found between the overexpression strain and the control strain. Therefore, this study highlights the gene expression regulation within mevalonate (MVA), ergosterol biosynthesis, and fatty acid biosynthesis pathways.

Aspergillus oryzae atfB encodes a transcription factor required for stress tolerance in conidia

2008 ◽  
Vol 45 (6) ◽  
pp. 922-932 ◽  
Author(s):  
Kazutoshi Sakamoto ◽  
Toshi-hide Arima ◽  
Kazuhiro Iwashita ◽  
Osamu Yamada ◽  
Katsuya Gomi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Tolerance ◽  
Aspergillus Oryzae

AoRim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus Aspergillus oryzae

2016 ◽  
Vol 121 (4) ◽  
pp. 365-371 ◽  
Author(s):  
Hidetoshi Nakamura ◽  
Takashi Kikuma ◽  
Feng Jie Jin ◽  
Jun-ichi Maruyama ◽  
Katsuhiko Kitamoto
Keyword(s):  
Stress Tolerance ◽  
Aspergillus Oryzae ◽  
Filamentous Fungus

Aspergillus oryzae atfA controls conidial germination and stress tolerance

2009 ◽  
Vol 46 (12) ◽  
pp. 887-897 ◽  
Author(s):  
Kazutoshi Sakamoto ◽  
Kazuhiro Iwashita ◽  
Osamu Yamada ◽  
Ken Kobayashi ◽  
Akihiro Mizuno ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Aspergillus Oryzae ◽  
Conidial Germination

scholarly journals Identification of Six Thiolases and their Effects on Fatty Acid and Ergosterol Biosynthesis in Aspergillus oryzae

2021 ◽  
Author(s):  
Hui Huang ◽  
Yali Niu ◽  
Qi Jin ◽  
Kunhai Qin ◽  
Li Wang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Genetic Engineering ◽  
Aspergillus Oryzae ◽  
Bioinformatics Analysis ◽  
Lipid Production ◽  
Ergosterol Biosynthesis ◽  
Dual Function ◽  
Growth Speed ◽  
Growth Stages

AbstractThiolase plays important roles in lipid metabolism. It can be divided into degradative thiolases (Thioase I) and biosynthetic thiolases (thiolases II), which are involved in fatty acid β-oxidation and acetoacetyl-CoA biosynthesis, respectively. The Saccharomyces cerevisiae (S. cerevisiae) genome harbors only one gene each for thioase I and thiolase II, namely, Pot1 and Erg10, respectively. In this study, six thiolases (named AoErg10A−AoErg10F) were identified in Aspergillus oryzae (A. oryzae) genome using bioinformatics analysis. Quantitative reverse transcription–PCR (qRT-PCR) indicated that the expression of these six thiolases varied at different growth stages and under different forms of abiotic stress. Subcellular localization analysis showed that AoErg10A was located in the cytoplasm, AoErg10B and AoErg10C in the mitochondria, and AoErg10D-AoErg10F in the peroxisome. Yeast heterologous complementation assays revealed that AoErg10A, AoErg10D, AoErg10E, AoErg10F and cytoplasmic AoErg10B (AoErg10BΔMTS) recovered the phenotypes of S. cerevisiae erg10 weak and lethal mutants, and that only AoErg10D-F recovered the phenotype of the pot1 mutant that cannot use oleic acid as the carbon source. Overexpression of AoErg10s either affected the growth speed or sporulation of the transgenic strains. In addition, the fatty acid and ergosterol content changed in all the AoErg10-overexpressing strains. This study revealed the function of six thiolases in A. oryzae and their effect on growth, and fatty acid and ergosterol biosynthesis, which may lay the foundation for genetic engineering for lipid metabolism in A. oryzae or other fungi.ImportanceThiolase including thioase I and thiolase II, plays important roles in lipid metabolism. A. oryzae, one of the most industrially important filamentous fungi, has been widely used for manufacturing oriental fermented food such as sauce, miso, and sake for a long time. Besides, A. oryzae has a high capability in production of high lipid content and has been used for lipid production. Thus, it is very important to investiagte the function of thiolases in A. oryzae. In this study, six thiolase (named AoErg10A-AoErg10F) were identified by bioinformatics analysis. Unlike other reported thiolases in fungi, three of the six thiolases showed dual function of thioase I and thiolase II in S. cerevisiae, indicating the lipid metabolism is more complex in A. oryzae. The reveal of founction of these thiolases in A. oryzae can lay the foundation for genetic engineering for lipid metabolism in A. oryzae or other fungi.

Investigating stress tolerance as a predictor of multitasking performance

2009 ◽  
Author(s):  
Ted B. Kinney ◽  
Mei-Chuan Kung ◽  
Kathleen M. Meckley ◽  
Kristin M. Delgado
Keyword(s):  
Stress Tolerance
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