scholarly journals Bacillus cereus Isolated From Vegetables in China: Incidence, Genetic Diversity, Virulence Genes, and Antimicrobial Resistance

2019 ◽  
Vol 10 ◽  
Author(s):  
Pengfei Yu ◽  
Shubo Yu ◽  
Juan Wang ◽  
Hui Guo ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Bacillus Cereus ◽  
Virulence Genes

scholarly journals Genetic diversity, antimicrobial resistance, and virulence genes of thermophilic Campylobacter isolated from broiler production chain

2020 ◽  
Vol 51 (4) ◽  
pp. 2021-2032
Author(s):  
Tassiana Ramires ◽  
Mauricéia Greici de Oliveira ◽  
Natalie Rauber Kleinubing ◽  
Simone de Fátima Rauber Würfel ◽  
Marcia Magalhães Mata ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Production Chain ◽  
Broiler Production ◽  
Thermophilic Campylobacter

scholarly journals Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products

2021 ◽  
Vol 9 (4) ◽  
Author(s):  
Angelica Bianco ◽  
Loredana Capozzi ◽  
Angela Miccolupo ◽  
Simona Iannetti ◽  
Maria Luisa Danzetta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Bacillus Cereus ◽  
Dairy Products ◽  
Virulence Genes ◽  
Food Poisoning ◽  
Important Food ◽  
Food Contaminants ◽  
Bacillus Cereus Group ◽  
Mlst Analysis ◽  
Sequence Types

Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products.

scholarly journals Prevalence, Antimicrobial Resistance, Virulence Genes and Genetic Diversity of Salmonella Isolated from Retail Duck Meat in Southern China

2020 ◽  
Vol 8 (3) ◽  
pp. 444
Author(s):  
Zhengquan Chen ◽  
Jie Bai ◽  
Shaojun Wang ◽  
Xibin Zhang ◽  
Zeqiang Zhan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Southern China ◽  
Public Health Issue ◽  
Serotype Distribution ◽  
Retail Markets ◽  
High Prevalence ◽  
Salmonella Serotypes ◽  
Potential Public Health

Salmonella is an important cause of foodborne diseases. This study was undertaken to investigate the prevalence, serotype distribution, antimicrobial resistance, virulence genes, and genetic diversity of Salmonella isolates recovered from fresh duck meat obtained from retail markets in Southern China. In total, 365 samples of fresh duck meat were collected from retail markets in six different cities of Guangdong Province between May 2017 and April 2019. High levels of Salmonella contamination were detected in duck meat (151/365, 41.4%). Twenty-six different Salmonella serotypes were identified: S. Corvallis (n = 25, 16.6%), S. Kentucky (n = 22, 14.6%) and S. Agona (n = 20, 13.3%) were the most prevalent serotypes. All isolates were resistant to at least one antibiotic and 133 (88.1%) isolates exhibited multidrug resistance (MDR). Most (86.1%) Salmonella isolates carried seven classes of virulence-associated genes. This study showed the diversity of Salmonella serotypes and genotypes and the high prevalence of MDR isolates carrying multiple virulence-associated genes among isolates from duck meat obtained from retail markets in Southern China. Isolates from different districts had similar pulsed-field gel electrophoresis (PFGE) patterns indicating that circulating foodborne Salmonella constitutes a potential public health issue across different districts.

scholarly journals Prevalence, virulence genes, and genetic diversity of Bacillus cereus isolated from convenience food [pdf]

2021 ◽  
Vol 20 (1) ◽  
pp. 113-120
Author(s):  
Alicja Dłubała ◽  
◽  
Elżbieta Bogusławska-Wąs ◽  
Elżbieta Daczkowska-Kozon
Keyword(s):  
Genetic Diversity ◽  
Bacillus Cereus ◽  
Virulence Genes ◽  
Convenience Food

scholarly journals Antimicrobial Resistance Profiles, Virulence Genes, and Genetic Diversity of Thermophilic Campylobacter Species Isolated From a Layer Poultry Farm in Korea

2021 ◽  
Vol 12 ◽  
Author(s):  
Noel Gahamanyi ◽  
Dae-Geun Song ◽  
Kye-Yoon Yoon ◽  
Leonard E. G. Mboera ◽  
Mecky I. Matee ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
South Korea ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Campylobacter Coli ◽  
Poultry Farm ◽  
Virulence Potential ◽  
Chicken Feces ◽  
High Level

Thermophilic Campylobacter species are among the major etiologies of bacterial enteritis globally. This study aimed at assessing the antimicrobial resistance (AMR) profiles, virulence genes, and genetic diversity of thermophilic Campylobacter species isolated from a layer poultry farm in South Korea. One hundred fifty-three chicken feces were collected from two layer poultry farms in Gangneung, South Korea. The Campylobacter species were isolated by cultural techniques, while PCR and sequencing were used for species confirmation. Antimicrobial susceptibility testing for six antimicrobials [ciprofloxacin (CIP), nalidixic acid (NAL), sitafloxacin (SIT), erythromycin (ERY), tetracycline (TET), and gentamicin (GEN)] was carried out by broth microdilution. Three AMR and nine virulence genes were screened by PCR. Genotyping was performed by flaA-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). Of the 153 samples, Campylobacter spp. were detected in 55 (35.9%), with Campylobacter jejuni and Campylobacter coli being 49 (89.1%) and six (10.9%), respectively. High-level resistance was observed for CIP (100%), NAL (100%), and TET (C. jejuni, 93.9%; C. coli: 83.3%). No resistance was observed for SIT. The missense mutation (C257T) in gyrA gene was confirmed by sequencing, while the tet(O) gene was similar to known sequences in GenBank. The rate of multidrug-resistant (MDR) strains was 8.2%, and they all belonged to C. jejuni. All Campylobacter isolates possessed five virulence genes (cdtB, cstII, flaA, cadF, and dnaJ), but none possessed ggt, while the rates for other genes (csrA, ciaB, and pldA) ranged between 33.3 and 95.9%. The flaA-RFLP yielded 26 flaA types (C. jejuni: 21 and C. coli: five), while the MLST showed 10 sequence types (STs) for C. jejuni and three STs for C. coli, with CC-607 (STs 3611) and CC-460 (ST-460) being predominant. Among the 10 STs of C. jejuni, three were newly assigned. The findings of this study highlight the increased resistance to quinolones and TET, the virulence potential, and the diverse genotypes among Campylobacter strains isolated from the layer poultry farm.

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