scholarly journals Detection and Identification of Bacillus cereus, Bacillus cytotoxicus, Bacillus thuringiensis, Bacillus mycoides and Bacillus weihenstephanensis via Machine Learning Based FTIR Spectroscopy

2019 ◽  
Vol 10 ◽  
Author(s):  
Murat Bağcıoğlu ◽  
Martina Fricker ◽  
Sophia Johler ◽  
Monika Ehling-Schulz
Keyword(s):  
Machine Learning ◽  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Ftir Spectroscopy ◽  
Bacillus Mycoides ◽  
Detection And Identification ◽  
Bacillus Weihenstephanensis

scholarly journals Multiple-Locus Sequence Typing Analysis of Bacillus cereus and Bacillus thuringiensis Reveals Separate Clustering and a Distinct Population Structure of Psychrotrophic Strains

2006 ◽  
Vol 72 (2) ◽  
pp. 1569-1578 ◽  
Author(s):  
Alexei Sorokin ◽  
Benjamin Candelon ◽  
Kévin Guilloux ◽  
Nathalie Galleron ◽  
Natalia Wackerow-Kouzova ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Individual Gene ◽  
Mild Winter ◽  
Systematic Testing ◽  
Bacillus Weihenstephanensis ◽  
Paris Area ◽  
Population Structures ◽  
Multiple Strains ◽  
Almost All

ABSTRACT We used multilocus sequence typing (MLST) to characterize phylogenetic relationships for a collection of Bacillus cereus group strains isolated from forest soil in the Paris area during a mild winter. This collection contains multiple strains isolated from the same soil sample and strains isolated from samples from different sites. We characterized 115 strains of this collection and 19 other strains based on the sequences of the clpC, dinB, gdpD, panC, purF, and yhfL loci. The number of alleles ranged from 36 to 53, and a total of 93 allelic profiles or sequence types were distinguished. We identified three major strain clusters—C, T, and W—based on the comparison of individual gene sequences or concatenated sequences. Some less representative clusters and subclusters were also distinguished. Analysis of the MLST data using the concept of clonal complexes led to the identification of two, five, and three such groups in clusters C, T, and W, respectively. Some of the forest isolates were closely related to independently isolated psychrotrophic strains. Systematic testing of the strains of this collection showed that almost all the strains that were able to grow at a low temperature (6°C) belonged to cluster W. Most of these strains, including three independently isolated strains, belong to two clonal complexes and are therefore very closely related genetically. These clonal complexes represent strains corresponding to the previously identified species Bacillus weihenstephanensis. Most of the other strains of our collection, including some from the W cluster, are not psychrotrophic. B. weihenstephanensis (cluster W) strains appear to comprise an effectively sexual population, whereas Bacillus thuringiensis (cluster T) and B. cereus (cluster C) have clonal population structures.

Inhibition of Bacillus anthracis and Potential Surrogate Bacilli Growth from Spore Inocula by Nisin and Other Antimicrobial Peptides

2006 ◽  
Vol 69 (10) ◽  
pp. 2529-2533 ◽  
Author(s):  
THOMAS J. MONTVILLE ◽  
TARA DE SIANO ◽  
ADAM NOCK ◽  
SALLY PADHI ◽  
DAVID WADE
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Thuringiensis ◽  
Antimicrobial Peptides ◽  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Bacillus Mycoides ◽  
Magainin Ii ◽  
Bacillus Strains

The ability of nisin, synthetic temporin analogs, magainins, defensins, and cecropins to inhibit Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, and Bacillus subtilis growth from spore inocula was determined using well diffusion assays. Nisin, magainin II amide, and defensins were inhibitory in screening against B. anthracis Sterne or B. cereus ATCC 7004, but only nisin inhibited virulent B. anthracis strains. The MICs of nisin against the 10 Bacillus strains examined were 0.70 to 13.51 μg/ml. Synthetic temporin analogs also inhibited B. anthracis but were not as potent as nisin. None of the strains examined were appropriate B. anthracis surrogates for testing sensitivity to antimicrobial peptides.

Bacillus cereus, Bacillus thuringiensis and Bacillus mycoides differentiation using a PCR-RE technique

2003 ◽  
Vol 81 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Marisa Manzano ◽  
Luca Cocolin ◽  
Carlo Cantoni ◽  
Giuseppe Comi
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Bacillus Mycoides

scholarly journals Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wilt disease on chili pepper

2018 ◽  
Vol 19 (4) ◽  
pp. 1532-1538 ◽  
Author(s):  
YULMIRA YANTI ◽  
WARNITA WARNITA ◽  
REFLIN REFLIN ◽  
MUNZIR BUSNIAH
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Chili Pepper ◽  
Strain Atcc ◽  
Bacillus Mycoides ◽  
Indigenous Bacteria ◽  
Bacillus Spp ◽  
Thuringiensis Strain

Yanti Y, Warnita, Reflin, Busniah M. 2018. Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wiltdisease on chili pepper. Biodiversitas 19: 1532-1538. Bacterial wilt and Fusarium wilt caused by Ralstonia syzigii subsp. indonesiensisand Fusarium oxysporum f.sp. capsici (Foc), respectively are the most damaging vascular pathogens in chili pepper (Capsicum annuumL.) and many other crops limiting their production, worldwide. Various strategies have been developed to control wilt pathogensincluding the application of chemical pesticides, which generally considered as the most effective and fastest strategy for plant diseasemanagement. However, effective chemicals for wilt pathogens of chili pepper plants are not available, yet. Endophytic bacteriaconsidered as one of options to control vascular wilt disease because of its ability to live and colonize in internal roots of plants.Previous research has been done to select endophytic indigenous bacteria isolates which can promote growth rate of chili pepper. Thepurpose of the research was to identify the selected indigenous endophyte bacteria isolates acquired from our previous study using 16SrRNA identifications and to screen the selected endophytic indigenous bacteria to control both R. syzigii subsp. indonesiensis and Foc.Results from 16S r RNA analysis showed that all of 9 isolates were identified as Bacillus spp., such as Bacillus cereus ATCC 14579,Bacillus pseudomycoides strain NBRC 101232, Bacillus toyonensis strain BCT-7112, Bacillus thuringiensis strain ATCC 10792,Bacillus weihenstephanensis strain DSM 11821, Bacillus mycoides strain 273, Bacillus cereus strain NBRC 15305, Bacillusbingmayongensis strain FJAT-13831 and Bacillus manliponensis strain BL4-6. Our results showed that most of endophytic bacteriaisolates application could control both bacterial and Fusarium wilt diseases. Six out of nine isolates can suppress R. syzigii subsp.indonesiensis without developing any symptoms and five isolates could suppress symptoms of Foc. Isolates Bacillus pseudomycoidesstrain NBRC 101232 Bacillus thuringiensis strain ATCC 10792 and Bacillus mycoides strain 273 were potential for control Foc and R.syzigii subsp. indonesiensis in chili pepper.

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