scholarly journals Probing Clostridium difficile Infection in Complex Human Gut Cellular Models

2019 ◽  
Vol 10 ◽  
Author(s):  
Blessing O. Anonye ◽  
Jack Hassall ◽  
Jamie Patient ◽  
Usanee Detamornrat ◽  
Afnan M. Aladdad ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Human Gut ◽  
Cellular Models

scholarly journals Evaluation of linezolid for the treatment of Clostridium difficile infection caused by epidemic strains using an in vitro human gut model

2011 ◽  
Vol 66 (7) ◽  
pp. 1537-1546 ◽  
Author(s):  
S. D. Baines ◽  
A. R. Noel ◽  
G. S. Huscroft ◽  
S. L. Todhunter ◽  
R. O'Connor ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Human Gut

scholarly journals Effectiveness of a short (4 day) course of oritavancin in the treatment of simulated Clostridium difficile infection using a human gut model

2012 ◽  
Vol 67 (10) ◽  
pp. 2434-2437 ◽  
Author(s):  
C. H. Chilton ◽  
J. Freeman ◽  
G. S. Crowther ◽  
S. L. Todhunter ◽  
M. H. Wilcox
Keyword(s):  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Human Gut

scholarly journals Mecillinam: a low-risk antimicrobial agent for induction of Clostridium difficile infection in an in vitro human gut model

2009 ◽  
Vol 63 (4) ◽  
pp. 838-839 ◽  
Author(s):  
S. D. Baines ◽  
R. O'Connor ◽  
G. Huscroft ◽  
K. Saxton ◽  
J. Freeman ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Antimicrobial Agent ◽  
Clostridium Difficile Infection ◽  
Low Risk ◽  
Human Gut

scholarly journals Evaluation of NVB302 versus vancomycin activity in an in vitro human gut model of Clostridium difficile infection

2012 ◽  
Vol 68 (1) ◽  
pp. 168-176 ◽  
Author(s):  
G. S. Crowther ◽  
S. D. Baines ◽  
S. L. Todhunter ◽  
J. Freeman ◽  
C. H. Chilton ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Human Gut

scholarly journals Probing Clostridium difficile infection in innovative human gut cellular models

2018 ◽  
Author(s):  
Blessing O. Anonye ◽  
Jack Hassall ◽  
Jamie Patient ◽  
Usanee Detamornrat ◽  
Afnan M. Aladdad ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Epithelial Cells ◽  
Gradual Increase ◽  
Controlled Environment ◽  
Human Gut ◽  
Epithelial Monolayers ◽  
Cellular Models ◽  
Gut Colonization ◽  
D Model

AbstractInteractions of anaerobic gut bacteria, such as Clostridium difficile, with the intestinal mucosa have been poorly studied due to challenges in culturing anaerobes with the oxygen-requiring gut epithelium. Although gut colonization by C. difficile is a key determinant of disease outcome, precise mechanisms of mucosal attachment and spread remain unclear. Here, using human gut epithelial monolayers co-cultured within dual environment chambers, we demonstrate that C. difficile adhesion to gut epithelial cells is accompanied by a gradual increase in bacterial numbers. Prolonged infection causes redistribution of actin and loss of epithelial integrity, accompanied by production of C. difficile spores, toxins and bacterial filaments. This 2-D dual chamber system was used to examine C. difficile interactions with the commensal Bacteroides dorei, and interestingly, C. difficile growth is significantly reduced in presence of B. dorei. Furthermore, in novel multilayer and 3-D gut models containing a myofibroblast layer, C. difficile adheres more efficiently to epithelial cells, as compared to the 2-D model, leading to a quicker destruction of the epithelium. Our study describes new controlled environment human gut models that enable host-anaerobe and pathogen-commensal interaction studies in vitro.

Self-Administered Home Series Fecal “Minitransplants” for Recurrent Clostridium difficile Infection on a Rectal Remnant

2015 ◽  
Vol 24 (4) ◽  
pp. 531-533 ◽  
Author(s):  
Daniel Popa ◽  
Mihaela Laszlo ◽  
Lidia Ciobanu ◽  
Elena Ucenic ◽  
Manuela Mihalache ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Toxic Megacolon ◽  
Fecal Microbiota ◽  
Recurrent Infections ◽  
Resonance Imaging ◽  
Vaginal Infection ◽  
Fecal Microbiota Transplant ◽  
Recurrent Clostridium Difficile Infection

A fecal microbiota transplant has proved to be an extremely effective method for patients with recurrent infections with Clostridium difficile. We present the case of a 65-year-old female patient with multiple Clostridium difficile infection (CDI) relapses on the rectal remnant, post-colectomy for a CDI-related toxic megacolon. The patient also evidenced associated symptomatic Clostridium difficile vaginal infection. She was successfully treated with serial fecal “minitransplants” (self-administered at home) and metronidazole ovules.Abbreviations: GI: gastrointestinal; MRI: magnetic resonance imaging; CDI: Clostridium difficile infection; FMT: fecal microbiota transplant.

Serendipity in Refractory Celiac Disease: Full Recovery of Duodenal Villi and Clinical Symptoms after Fecal Microbiota Transfer

2016 ◽  
Vol 25 (3) ◽  
pp. 385-388 ◽  
Author(s):  
Yvette H. Van Beurden ◽  
Tom Van Gils ◽  
Nienke A. Van Gils ◽  
Zain Kassam ◽  
Chris J.J. Mulder ◽  
...  
Keyword(s):  
Celiac Disease ◽  
T Cell ◽  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Cell Lymphoma ◽  
Fecal Microbiota ◽  
Disease Type ◽  
T Cell Lymphoma ◽  
Type Ii ◽  
Refractory Celiac Disease

Treatment of refractory celiac disease type II (RCD II) and preventing the development of an enteropathy associated T-cell lymphoma in these patients is still difficult. In this case report, we describe a patient with RCD II who received fecal microbiota transfer as treatment for a recurrent Clostridium difficile infection, and remarkably showed a full recovery of duodenal villi and disappearance of celiac symptoms. This case suggests that altering the gut microbiota may hold promise in improving the clinical and histological consequences of celiac disease and/or RCD II. Abbreviations: CDI: Clostridium difficile infection; EATL : enteropathy associated T-cell lymphoma; FMT: fecal microbiota transfer; IEL: intraepithelial lymphocytes; RCD II: refractory celiac disease type II; TPN: total parenteral nutrition.

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