scholarly journals Development of a Blocking Primer to Inhibit the PCR Amplification of the 18S rDNA Sequences of Litopenaeus vannamei and Its Efficacy in Crassostrea hongkongensis

2019 ◽  
Vol 10 ◽  
Author(s):  
Cong Liu ◽  
Ren-Jie Qi ◽  
Jing-Zhe Jiang ◽  
Ming-Qing Zhang ◽  
Jiang-Yong Wang
Keyword(s):  
Litopenaeus Vannamei ◽  
18S Rdna ◽  
Pcr Amplification ◽  
Crassostrea Hongkongensis ◽  
Rdna Sequences ◽  
Blocking Primer ◽  
18S Rdna Sequences

Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows1

2003 ◽  
Vol 81 (3) ◽  
pp. 812-815 ◽  
Author(s):  
S. K. R. Karnati ◽  
Z. Yu ◽  
J. T. Sylvester ◽  
B. A. Dehority ◽  
M. Morrison ◽  
...  
Keyword(s):  
18S Rdna ◽  
Pcr Amplification ◽  
Technical Note ◽  
Specific Pcr ◽  
Rdna Sequences ◽  
18S Rdna Sequences

scholarly journals Phylogeny of the Thoracican Barnacles Based on 18S rDNA Sequences

2000 ◽  
Vol 20 (2) ◽  
pp. 393-398 ◽  
Author(s):  
D. James Harris ◽  
Laura S. Maxson ◽  
Lee F. Braithwaite ◽  
Keith A. Crandall
Keyword(s):  
18S Rdna ◽  
Rdna Sequences ◽  
18S Rdna Sequences

Assessing the Biodiversity of Monoraphidium Using 18S rDNA Sequences

2002 ◽  
Vol 38 (s1) ◽  
pp. 9-9
Author(s):  
M. W. Fawley ◽  
K. P. Fawley ◽  
M. L. Dean
Keyword(s):  
18S Rdna ◽  
Rdna Sequences ◽  
18S Rdna Sequences

scholarly journals Sarcocystis Attenuati N. Sp. (Apicomplexa: Sarcocystidae) Infecting the Asian Gray Shrew, Crocidura Attenuata (Insectivora: Soricidae), in China

2021 ◽  
Author(s):  
junjie hu ◽  
Jun Sun ◽  
Yanmei Guo ◽  
Hongxia Zeng ◽  
Yunzhi Zhang ◽  
...  
Keyword(s):  
Host Specificity ◽  
Definitive Host ◽  
18S Rdna ◽  
Common Species ◽  
Molecular Characteristics ◽  
Intermediate Hosts ◽  
Parasite Life Cycle ◽  
Rdna Sequences ◽  
Transmission Electron ◽  
18S Rdna Sequences

Abstract Background: There are limited data on Sarcocystis in insectivores. The Asian gray shrew, Crocidura attenuata, is one of the most common species of insectivores in the family Soricidae distributed in South Asia and Southeast Asia. To date, Sarcocystis has never been recorded in this host.Methods: Tissues from 42 Asian gray shrews were collected in China in 2017 and 2018. Sarcocysts were observed using light (LM) and transmission electron microscopy (TEM). To complete the parasite life cycle, muscle tissues of the host infected with sarcocysts were force-fed to two beauty rat snakes, Elaphe taeniura. Individual sarcocysts from different Asian gray shrews and oocysts/sporocysts isolated from the small intestines and feces of the experimental snakes were selected for DNA extraction, and seven genetic markers, including two nuclear loci (18S rDNA and ITS1), three mitochondrial genes (cox1, cox3 and cytb), and two apicoplastic genes (rpoB and clpC), were amplified, sequenced and analyzed.Results: Sarcocysts were found in 17 of 42 (40.5%) Asian gray shrews. Under LM, the microscopic sarcocysts were exhibited saw-tooth-like protrusions measuring 3.3–4.5 μm. Ultrastructurally, the sarcocyst wall contained numerous lancet- or leaf-like villous protrusions, similar to type 9h. The experimental beauty rat snakes shed oocysts/sporcysts measuring 11.9–16.7 × 9.2–10.6 μm with a prepatent period of 10 to 11 days. Comparing these sequences with those previously deposited in GenBank revealed that the 18S rDNA sequences and cox1 sequences shared the highest similarity with those of S. scandentiborneensis recorded in tree shrews, Tuaia minor and T. tana (i.e., 97.6–98.3% and 100% identity, respectively). Phylogenetic analysis based on 18S rDNA, ITS1 or cox1 sequences revealed that this parasite formed an independent clade with Sarcocystis spp. that utilize small animals as intermediate hosts and snakes as the known or presumed definitive host. On the basis of morphological and molecular characteristics and host specificity, the parasite was proposed as a new species, named S. attenuati.Conclusions: Sarcocysts were recorded in Asian gray shrews for the first time. The sarcocysts were characterized morphologically and molecularly. The 18S rDNA and cox1 sequences of S. attenuati, named in the present study, shared the highest identities with those of S. scandentiborneensis. However, the sarcocysts of the two species of Sarcocystis were quite different under LM and TEM. Based on experimental infection, beauty rat snakes have been proven to be a definitive host of S. attenuati. As more species of Sarcocystis from insectivores and other small mammals are properly morphologically and molecularly characterized, we may gain a better understanding of the biodiversity, host specificity and evolution of Sarcocystis in the future.

scholarly journals Unexplored diversity of microscopic myxomycetes: evidence from environmental DNA

2019 ◽  
Vol 152 (3) ◽  
pp. 499-506 ◽  
Author(s):  
Oleg N. Shchepin ◽  
Martin Schnittler ◽  
Nikki H.A. Dagamac ◽  
Dmitry V. Leontyev ◽  
Yuri K. Novozhilov
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Environmental Dna ◽  
Full Length ◽  
Detection Techniques ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
Environmental Sequences

Background and aims – Recent studies showed the position of two slime mould species with microscopic sporocarps, Echinosteliopsis oligospora and Echinostelium bisporum, within the class Myxomycetes. These minute species are seldom seen in studies based on detection of sporocarps and can easily be confused with protosteloid amoebozoans.Methods – We searched all published ePCR data sets that targeted myxomycete 18S rDNA for the presence of environmental sequences similar to E. oligospora and Echinosteliales in traditional circumscription, and performed phylogenetic analyses that included short environmental sequences and full-length 18S rDNA sequences representing all the major groups of myxomycetes.Key results – We report 19 unique sequences which are closely related to E. bisporum or E. oligospora based on sequence similarity (73.1–95.2% similarity) and which form well-supported monophyletic clades with these species in phylogenetic analyses. They may represent new species that are not yet described. Our phylogeny based on full-length 18S rDNA sequences further confirms the position of E. bisporum and E. oligospora within myxomycetes and the paraphyly of the order Echinosteliales in its traditional circumscription.Conclusions – Our results show that ePCR-based studies can reveal myxomycete taxa that often escape detection by traditional approaches, including potentially new species, and thus provide valuable new data on diversity and ecology of myxomycetes. As such, strategies for studying myxomycetes biodiversity should be revised, focusing also on molecular detection techniques in addition to the sporocarp-based ones.

Eimeria trichosuri: Phylogenetic position of a marsupial coccidium, based on 18S rDNA sequences

2009 ◽  
Vol 122 (2) ◽  
pp. 165-168 ◽  
Author(s):  
M.L. Power ◽  
C. Richter ◽  
S. Emery ◽  
J. Hufschmid ◽  
M.R. Gillings
Keyword(s):  
18S Rdna ◽  
Phylogenetic Position ◽  
Rdna Sequences ◽  
18S Rdna Sequences

Myxobolus species infecting the cartilaginous rays of the gill filaments in cyprinid fishes

2008 ◽  
Vol 53 (4) ◽  
Author(s):  
Kálmán Molnár ◽  
Gábor Cech ◽  
Csaba Székely
Keyword(s):  
18S Rdna ◽  
Rutilus Rutilus ◽  
Molecular Methods ◽  
Roach Rutilus Rutilus ◽  
Myxobolus Species ◽  
Rdna Sequences ◽  
Gill Filaments ◽  
18S Rdna Sequences ◽  
Cyprinid Fishes ◽  
Bleak Alburnus Alburnus

AbstractDuring a survey on myxosporean parasites of cyprinid fishes in Hungary, Myxobolus infections were found in the cartilaginous rays of the gill filaments in roach (Rutilus rutilus) and bleak (Alburnus alburnus). Myxobolus spp. causing the infections were studied by morphological, histological and molecular methods. Small plasmodia surrounded by chondrocytes contained relatively few spores which differed from each other and from the known Myxobolus spp. both in their morphology and 18S rDNA sequences. Both species, described as M. feisti sp. nov. and M. susanlimae sp. nov., are characterised by a specific cartilaginous histotropism.

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