scholarly journals Potent Inhibition of Zika Virus Replication by Aurintricarboxylic Acid

2019 ◽  
Vol 10 ◽  
Author(s):  
Jun-Gyu Park ◽  
Ginés Ávila-Pérez ◽  
Ferralita Madere ◽  
Thomas A. Hilimire ◽  
Aitor Nogales ◽  
...  
Keyword(s):  
Virus Replication ◽  
Zika Virus ◽  
Aurintricarboxylic Acid ◽  
Potent Inhibition

scholarly journals Potent inhibition of Zika virus replication by curcumin - poly(sodium 4-styrenesulfonate) conjugates

2020 ◽  
Author(s):  
Magdalena Obłoza ◽  
Paweł Botwina ◽  
Gabriela Czerwonka ◽  
Krzysztof Szczubiałka ◽  
Maria Nowakowska ◽  
...  
Keyword(s):  
Virus Replication ◽  
Zika Virus ◽  
Potent Inhibition

The P-MAPA immunomodulator partially prevents apoptosis induced by Zika virus infection in THP-1 cells

2020 ◽  
Vol 21 ◽  
Author(s):  
Morganna C. Lima ◽  
Elisa A. N. Azevedo ◽  
Clarice N. L. de Morais ◽  
Larissa I. O. de Sousa ◽  
Bruno M. Carvalho ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Virus Infection ◽  
Virus Replication ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Caspase 3 ◽  
Neurological Complications ◽  
Zika Virus Infection

Background: Zika virus is an emerging arbovirus of global importance. ZIKV infection is associated with a range of neurological complications such as the Congenital Zika Syndrome and Guillain Barré Syndrome. Despite the magnitude of recent outbreaks, there is no specific therapy to prevent or to alleviate disease pathology. Objective: To investigate the role of P-MAPA immunomodulator in Zika-infected THP-1 cells. Methods: THP-1 cells were subjected at Zika virus infection (Multiplicity of Infection = 0.5) followed by treatment with P-MAPA for until 96 hours post-infection. After that, the cell death was analyzed by annexin+/ PI+ and caspase 3/ 7+ staining by flow cytometry. In addition, the virus replication and cell proliferation were accessed by RT-qPCR and Ki67 staining, respectively. Results: We demonstrate that P-MAPA in vitro treatment significantly reduces Zika virus-induced cell death and caspase-3/7 activation on THP-1 infected cells, albeit it has no role in virus replication and cell proliferation. Conclusions: Our study reveals that P-MAPA seems to be a satisfactory alternative to inhibits the effects of Zika virus infection in mammalian cells.

Valosin‐containing protein ATPase activity regulates the morphogenesis of Zika virus replication organelles and virus‐induced cell death

2021 ◽  
Author(s):  
Anaïs Anton ◽  
Clément Mazeaud ◽  
Wesley Freppel ◽  
Claudia Gilbert ◽  
Nicolas Tremblay ◽  
...  
Keyword(s):  
Cell Death ◽  
Atpase Activity ◽  
Virus Replication ◽  
Zika Virus

Further Characterization of the Potent and Prolonged Inhibition of Herpes Simplex Virus Replication in Human Cell Lines by Penciclovir

1996 ◽  
Vol 7 (3) ◽  
pp. 128-137 ◽  
Author(s):  
T.H. Bacon ◽  
B.A. Howard
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cell Lines ◽  
Virus Replication ◽  
Human Cell ◽  
Human Cell Lines ◽  
Potent Inhibition ◽  
Multiple Cycles ◽  
Simplex Virus ◽  
Hsv 1

The replication of herpes simplex virus type 1 (HSV-1) or HSV-2 in MRC-5 cells infected at 0.01 pfu cell−1 treated continuously for 72 h, was inhibited more efficiently by penciclovir than aciclovir ( p = 0.0001). However, multiple cycles of replication were required in order to distinguish the compounds. Virus from cultures treated for 72 h with either compound, at 3 or 10 μg ml−1 was resistant to penciclovir and aciclovir (50% effective concentrations > 10 μg ml−1), but infectivity titres of supernatants from these aciclovirtreated cultures were higher than for penciclovir. Increased production of resistant virus in aciclovirtreated cultures may be the consequence of the less potent inhibition of virus replication by aciclovir. Penciclovir caused prolonged inhibition of HSV-1 and HSV-2 replication in three human cell lines infected at 1 pfu cell−1 following treatment for 18 h, whereas virus replication resumed rapidly after withdrawal of aciclovir. Neither compound showed prolonged activity after 18 h treatment, when the multiplicity of infection was reduced to 0.01 pfu cell−1. This surprising observation prompted experiments testing the effect of repeated pulse treatment in cultures infected at low multiplicity. Penciclovir inhibited HSV-1 replication significantly more effectively than aciclovir in MRC-5 cells infected at 10−4 pfu cell−1 treated daily for 6 h ( p < 0.001, n = 5) but only a trend was observed for HSV-2 ( p = 0.06, n = 6).

Antiviral activity of pinocembrin against Zika virus replication

2019 ◽  
Vol 167 ◽  
pp. 13-24 ◽  
Author(s):  
Jia Le Lee ◽  
Marcus Wing Choy Loe ◽  
Regina Ching Hua Lee ◽  
Justin Jang Hann Chu
Keyword(s):  
Antiviral Activity ◽  
Virus Replication ◽  
Zika Virus

scholarly journals Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures

2017 ◽  
Vol 91 (14) ◽  
Author(s):  
Michaela J. Schultz ◽  
Sharon Isern ◽  
Scott F. Michael ◽  
Ronald B. Corley ◽  
John H. Connor ◽  
...  
Keyword(s):  
Virus Replication ◽  
Zika Virus ◽  
Western Hemisphere ◽  
Mosquito Cell ◽  
Mosquito Vector ◽  
Viral Inhibition ◽  
Virus Growth ◽  
Content Type ◽  
Bacterial Endosymbiont ◽  
Mosquito Cells

ABSTRACT Mosquito-borne arboviruses are a major source of human disease. One strategy to reduce arbovirus disease is to reduce the mosquito's ability to transmit virus. Mosquito infection with the bacterial endosymbiont Wolbachia pipientis wMel is a novel strategy to reduce Aedes mosquito competency for flavivirus infection. However, experiments investigating cyclic environmental temperatures have shown a reduction in maternal transmission of wMel, potentially weakening the integration of this strain into a mosquito population relative to that of other Wolbachia strains. Consequently, it is important to investigate additional Wolbachia strains. All Zika virus (ZIKV) suppression studies are limited to the wMel Wolbachia strain. Here we show ZIKV inhibition by two different Wolbachia strains: wAlbB (isolated from Aedes albopictus mosquitoes) and wStri (isolated from the planthopper Laodelphax striatellus) in mosquito cells. Wolbachia strain wStri inhibited ZIKV most effectively. Single-cycle infection experiments showed that ZIKV RNA replication and nonstructural protein 5 translation were reduced below the limits of detection in wStri-containing cells, demonstrating early inhibition of virus replication. ZIKV replication was rescued when Wolbachia was inhibited with a bacteriostatic antibiotic. We observed a partial rescue of ZIKV growth when Wolbachia-infected cells were supplemented with cholesterol-lipid concentrate, suggesting competition for nutrients as one of the possible mechanisms of Wolbachia inhibition of ZIKV. Our data show that wAlbB and wStri infection causes inhibition of ZIKV, making them attractive candidates for further in vitro mechanistic and in vivo studies and future vector-centered approaches to limit ZIKV infection and spread. IMPORTANCE Zika virus (ZIKV) has swiftly spread throughout most of the Western Hemisphere. This is due in large part to its replication in and spread by a mosquito vector host. There is an urgent need for approaches that limit ZIKV replication in mosquitoes. One exciting approach for this is to use a bacterial endosymbiont called Wolbachia that can populate mosquito cells and inhibit ZIKV replication. Here we show that two different strains of Wolbachia, wAlbB and wStri, are effective at repressing ZIKV in mosquito cell lines. Repression of virus growth is through the inhibition of an early stage of infection and requires actively replicating Wolbachia. Our findings further the understanding of Wolbachia viral inhibition and provide novel tools that can be used in an effort to limit ZIKV replication in the mosquito vector, thereby interrupting the transmission and spread of the virus.

scholarly journals Human Sertoli cells support high levels of Zika virus replication and persistence

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Anil Kumar ◽  
Juan Jovel ◽  
Joaquin Lopez-Orozco ◽  
Daniel Limonta ◽  
Adriana M. Airo ◽  
...  
Keyword(s):  
Virus Replication ◽  
Sertoli Cells ◽  
Zika Virus

scholarly journals Toll-like receptor agonist R848 blocks Zika virus replication by inducing the antiviral protein viperin

Virology ◽  
2018 ◽  
Vol 522 ◽  
pp. 199-208 ◽  
Author(s):  
Bénédicte Vanwalscappel ◽  
Takuya Tada ◽  
Nathaniel R. Landau
Keyword(s):  
Virus Replication ◽  
Zika Virus ◽  
Receptor Agonist ◽  
Toll Like Receptor ◽  
Antiviral Protein

scholarly journals A global lipid map defines a network essential for Zika virus replication

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Hans C. Leier ◽  
Jules B. Weinstein ◽  
Jennifer E. Kyle ◽  
Joon-Yong Lee ◽  
Lisa M. Bramer ◽  
...  
Keyword(s):  
Virus Replication ◽  
Zika Virus
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