scholarly journals Quantitative Polymerase Chain Reaction Coupled With Sodium Dodecyl Sulfate and Propidium Monoazide for Detection of Viable Streptococcus agalactiae in Milk

2019 ◽  
Vol 10 ◽  
Author(s):  
Yankun Zhao ◽  
He Chen ◽  
Huimin Liu ◽  
Jianxing Cai ◽  
Lu Meng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sodium Dodecyl Sulfate ◽  
Streptococcus Agalactiae ◽  
Quantitative Polymerase Chain Reaction ◽  
Sodium Dodecyl ◽  
Propidium Monoazide ◽  
Chain Reaction ◽  
Dodecyl Sulfate ◽  
Polymerase Chain

scholarly journals ANALYSIS OF THE INHIBITORY POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM ADULT SALIVA AND THE TONGUE DORSUM FOR THE GROWTH OF CANDIDA ALBICANS

2017 ◽  
Vol 9 ◽  
pp. 3
Author(s):  
Nindy Fairiska ◽  
Boy M Bachtiar ◽  
Ferry P Gultom
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Streptococcus Salivarius ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Inhibitory Potential

Objectives: The objective of this study is to analyze the effectiveness of Streptococcus salivarius and its protein for inhibiting the growth of Candidaalbicans.Methods: The analysis was conducted using polymerase chain reaction, sodium dodecyl sulfate polyacrylamide gel electrophoresis, a Bradford test,deferred antagonism test, and well-diffusion agar.Result: S. salivarius, isolated from saliva and the tongue dorsum, and its protein do not inhibit the growth of C. albicans. The morphology of C. albicansdid not change after being exposed to protein produced by S. salivarius.Conclusions: S. salivarius and its protein do not inhibit the growth of C. albicans. However, the bacterium has the capacity to maintain fungusmorphology in the form of blastospora.

scholarly journals Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae

2021 ◽  
Author(s):  
Nastaran Hemmati ◽  
Farhad Nikkhahi ◽  
Amir Javadi ◽  
Sahar Eskandarion ◽  
Seyed Mahmoud Amin Marashi
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Streptococcus Pneumoniae ◽  
Bacterial Meningitis ◽  
Neisseria Meningitidis ◽  
Streptococcus Agalactiae ◽  
Predictive Value ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

Background and Objectives: Neisseria meningitidis, Escherichia coli K , Streptococcus agalactiae, and Streptococcus pneumoniae cause 90% of bacterial meningitis. Almost all infected people die or have irreversible neurological complica- tions. Therefore, it is essential to have a diagnostic kit with the ability to quickly detect these fatal infections. Materials and Methods: The project involved 212 patients from whom cerebrospinal fluid samples were obtained. After total genome extraction and performing multiplex quantitative polymerase chain reaction (qPCR), the presence or absence of each infectious factor was determined by comparing with standard strains. Results: The specificity, sensitivity, positive predictive value, and negative predictive value calculated were 100%, 92.9%, 50%, and 100%, respectively. So, due to the high specificity and sensitivity of the designed primers, they can be used instead of bacterial culture that takes at least 24 to 48 hours. Conclusion: The remarkable benefit of this method is associated with the speed (up to 3 hours) at which the procedure could be completed. It is also worth noting that this method can reduce the personnel unintentional errors which may occur in the laboratory. On the other hand, as this method simultaneously identifies four common factors that cause bacterial meningitis, it could be used as an auxiliary method diagnostic technique in laboratories particularly in cases of emergency medicine.

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