scholarly journals Single-Cell Transcriptome Analysis of CD34+ Stem Cell-Derived Myeloid Cells Infected With Human Cytomegalovirus

2019 ◽  
Vol 10 ◽  
Author(s):  
Melissa Galinato ◽  
Kristen Shimoda ◽  
Alexis Aguiar ◽  
Fiona Hennig ◽  
Dario Boffelli ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Human Cytomegalovirus ◽  
Transcriptome Analysis ◽  
Myeloid Cells ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals TAMI-26. SINGLE-CELL TRANSCRIPTOME ANALYSIS REVEALS THAT ENDOTHELIAL TRANSFORMATION DRIVES GLIOBLASTOMA CHEMORESISTANCE VIA Wnt/β-CATENIN/MRP-1

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii218-ii218
Author(s):  
Menggui Huang ◽  
Duo Zhang ◽  
Yi Fan
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Transcriptome Analysis ◽  
Therapeutic Resistance ◽  
Tumor Resistance ◽  
Genetic Ablation ◽  
Cytotoxic Treatment ◽  
Cell Transcriptome ◽  
Mesenchymal Transformation ◽  
Single Cell Transcriptome

Abstract Therapeutic resistance remains a persistent challenge for patients with glioblastoma (GBM). Here, we report that endothelial cells (ECs) acquire transformation into mesenchymal stem cell (MSC)-like cells in GBM, driving tumor resistance to cytotoxic treatment. Single-cell transcriptome analysis identified an EC-derived cell population that robustly expresses mesenchymal genes and stem cell-associated markers, suggesting that ECs undergo mesenchymal transformation and stemness-like activation in GBM microenvironment. Furthermore, we identified a c-Met-mediated axis that induces β-catenin phosphorylation at Ser675 and Wnt signaling activation, inducing multidrug resistance-associated protein-1 (MRP-1) expression and leading to EC stemness-like activation and chemoresistance. Last, genetic ablation of β-catenin in ECs overcome GBM tumor resistance to temozolomide (TMZ) chemotherapy in vivo. Combination of Wnt inhibition and TMZ chemotherapy eliminated tumor-associated ECs, inhibited GBM growth, and increased mouse survival. These findings identified a cell plasticity-based, microenvironment-dependent mechanism that controls tumor chemoresistance, and suggest that targeting Wnt/β-catenin-mediated EC transformation and stemness activation may overcome therapeutic resistance in GBM.

scholarly journals Single cell transcriptome analysis upon MCF-7 breast cancer

2010 ◽  
Vol 11 (Suppl 1) ◽  
pp. P8 ◽  
Author(s):  
Yih-Shien Chiang ◽  
Lock Seng ◽  
You-Yu Lin ◽  
Shih-Hao Chen ◽  
Yu-Chang Su ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Single Cell ◽  
Transcriptome Analysis ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome ◽  
Mcf 7

scholarly journals Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation

2019 ◽  
Vol 47 (9) ◽  
pp. 4539-4553 ◽  
Author(s):  
Minoru Kubo ◽  
Tomoaki Nishiyama ◽  
Yosuke Tamada ◽  
Ryosuke Sano ◽  
Masaki Ishikawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Transcriptome Analysis ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome
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