Global Transcriptional Profiling of the Cyanobacterium Chlorogloeopsis fritschii PCC 9212 in Far-Red Light: Insights Into the Regulation of Chlorophyll d Synthesis

Harvesting Far-Red Light with Plant Antenna Complexes Incorporating Chlorophyll d

Biomacromolecules ◽

10.1021/acs.biomac.1c00435 ◽

2021 ◽

Author(s):

Eduard Elias ◽

Nicoletta Liguori ◽

Yoshitaka Saga ◽

Judith Schäfers ◽

Roberta Croce

Keyword(s):

Red Light ◽

Chlorophyll D

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Far-Red Light Acclimation for Improved Mass Cultivation of Cyanobacteria

Metabolites ◽

10.3390/metabo9080170 ◽

2019 ◽

Vol 9 (8) ◽

pp. 170 ◽

Cited By ~ 3

Author(s):

Alla Silkina ◽

Bethan Kultschar ◽

Carole A. Llewellyn

Keyword(s):

Biomass Production ◽

White Light ◽

Light Emitting Diode ◽

Red Light ◽

Industrial Biotechnology ◽

Light Conditions ◽

Light Emitting ◽

Mass Cultivation ◽

Chlorogloeopsis Fritschii ◽

Bioactive Product

Improving mass cultivation of cyanobacteria is a goal for industrial biotechnology. In this study, the mass cultivation of the thermophilic cyanobacterium Chlorogloeopsis fritschii was assessed for biomass production under light-emitting diode white light (LEDWL), far-red light (FRL), and combined white light and far-red light (WLFRL) adaptation. The induction of chl f was confirmed at 24 h after the transfer of culture from LEDWL to FRL. Using combined light (WLFRL), chl f, a, and d, maintained the same level of concentration in comparison to FRL conditions. However, phycocyanin and xanthophylls (echinone, caloxanthin, myxoxanthin, nostoxanthin) concentration increased 2.7–4.7 times compared to LEDWL conditions. The productivity of culture was double under WLFRL compared with LEDWL conditions. No significant changes in lipid, protein, and carbohydrate concentrations were found in the two different light conditions. The results are important for informing on optimum biomass cultivation of this species for biomass production and bioactive product development.

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Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912

Scientific Reports ◽

10.1038/s41598-020-77402-6 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Carole A. Llewellyn ◽

Carolyn Greig ◽

Alla Silkina ◽

Bethan Kultschar ◽

Matthew D. Hitchings ◽

...

Keyword(s):

Amino Acid ◽

Gene Cluster ◽

White Light ◽

Aromatic Amino Acid ◽

Uv Light ◽

Red Light ◽

Ultra Violet ◽

Filamentous Cyanobacterium ◽

Chlorogloeopsis Fritschii ◽

The Impact

AbstractThe “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. The discovery that MAAs are upregulated under FR light supports MAAs playing a role in photon dissipation and thermoregulation with a possible role in contributing to Earth surface temperature regulation.

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Novel chlorophylls and new directions in photosynthesis research

Functional Plant Biology ◽

10.1071/fp14350 ◽

2015 ◽

Vol 42 (6) ◽

pp. 493 ◽

Cited By ~ 23

Author(s):

Yaqiong Li ◽

Min Chen

Keyword(s):

Chlorophyll A ◽

Red Light ◽

Oxygenic Photosynthesis ◽

Ecological Niches ◽

Absorption Bands ◽

Absorption Region ◽

Chlorophyll D ◽

And Function ◽

Unique Status ◽

Chlorophyll F

Chlorophyll d and chlorophyll f are red-shifted chlorophylls, because their Qy absorption bands are significantly red-shifted compared with chlorophyll a. The red-shifted chlorophylls broaden the light absorption region further into far red light. The presence of red-shifted chlorophylls in photosynthetic systems has opened up new possibilities of research on photosystem energetics and challenged the unique status of chlorophyll a in oxygenic photosynthesis. In this review, we report on the chemistry and function of red-shifted chlorophylls in photosynthesis and summarise the unique adaptations that have allowed the proliferation of chlorophyll d- and chlorophyll f-containing organisms in diverse ecological niches around the world.

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