scholarly journals Retraction: Molecular Cloning and Docking of speB Gene Encoding Cysteine Protease With Antibiotic Interaction in Streptococcus pyogenes NBMKU12 From the Clinical Isolates

2018 ◽  
Vol 9 ◽  
Author(s):  
Keyword(s):  
Molecular Cloning ◽  
Streptococcus Pyogenes ◽  
Cysteine Protease ◽  
Clinical Isolates ◽  
Gene Encoding

scholarly journals Molecular Cloning and Docking of speB Gene Encoding Cysteine Protease With Antibiotic Interaction in Streptococcus pyogenes NBMKU12 From the Clinical Isolates

2018 ◽  
Vol 9 ◽  
Author(s):  
Natesan Balasubramanian ◽  
Govintharaj Varatharaju ◽  
Vellasamy Shanmugaiah ◽  
Karuppiah Balakrishnan ◽  
Mandayam A. Thirunarayan
Keyword(s):  
Molecular Cloning ◽  
Streptococcus Pyogenes ◽  
Cysteine Protease ◽  
Clinical Isolates ◽  
Gene Encoding

scholarly journals Role of RopB in Growth Phase Expression of the SpeB Cysteine Protease of Streptococcus pyogenes

2003 ◽  
Vol 185 (17) ◽  
pp. 5166-5174 ◽  
Author(s):  
Melody N. Neely ◽  
William R. Lyon ◽  
Donna L. Runft ◽  
Michael Caparon
Keyword(s):  
Streptococcus Pyogenes ◽  
Growth Phase ◽  
Cysteine Protease ◽  
Ectopic Expression ◽  
Phase Control ◽  
Logarithmic Phase ◽  
Gene Encoding ◽  
Family Control ◽  
Phase Signal ◽  
Transcription Regulators

ABSTRACT The Rgg family of transcription regulators is widely distributed among gram-positive bacteria; however, how the members of this family control transcription is poorly understood. In the pathogen Streptococcus pyogenes, the Rgg family member RopB is required for transcription of the gene that encodes the secreted SpeB cysteine protease. Expression of the protease follows distinct kinetics that involves control of transcription in response to the growth phase. In this study, the contribution of RopB to growth phase control was examined. The gene encoding the protease (speB) and ropB are transcribed divergently from a 940-bp intergenic region. Primer extension analyses, in conjunction with reporter fusion studies, revealed that the major region controlling the transcription of both speB and ropB is adjacent to ropB and that the promoters for the two genes likely overlap. Furthermore, it was found that RopB is a DNA-binding protein that specifically binds to sequences in this control region. The interrelationship between ropB and speB expression was further reflected in the observation that transcription of ropB itself is subject to growth phase control. However, while expression of ropB from a promoter expressed during the early logarithmic phase of growth could complement a ropB deletion mutant, ectopic expression of ropB did not uncouple the expression of speB from its growth phase signal. These data implicate other factors in growth phase control and suggest that regulation of ropB expression itself is not the central mechanism of control.

Molecular cloning of the gene encoding RecF, a DNA repair enzyme, from Streptococcus pyogenes

Gene ◽  
1995 ◽  
Vol 156 (1) ◽  
pp. 89-91 ◽  
Author(s):  
Paul L. Deangelis ◽  
Ning Yang ◽  
Paul H. Weigel
Keyword(s):  
Dna Repair ◽  
Molecular Cloning ◽  
Streptococcus Pyogenes ◽  
Repair Enzyme ◽  
Gene Encoding

scholarly journals Effect of erythritol and xylitol on Streptococcus pyogenes causing peritonsillar abscesses

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Siiri Kõljalg ◽  
Risto Vaikjärv ◽  
Imbi Smidt ◽  
Tiiu Rööp ◽  
Anirikh Chakrabarti ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Bacterial Growth ◽  
Growth Curves ◽  
Culture Collection ◽  
Exponential Phase ◽  
Clinical Isolates ◽  
Growth Inhibitors ◽  
Throat Culture ◽  
Potent Growth

AbstractPolyols are effective against caries-causing streptococci but the effect on oropharynx-derived pyogenic streptococci is not well characterised. We aimed to study the effect of erythritol (ERY) and xylitol (XYL) against Streptococcus pyogenes isolated from peritonsillar abscesses (PTA). We used 31 clinical isolates and 5 throat culture collection strains. Inhibition of bacterial growth by polyols at 2.5%, 5% and 10% concentrations was studied and the results were scored. Amylase levels in PTA pus were compared to polyol effectivity scores (PES). Growth curves of four S. pyogenes isolates were analysed. Our study showed that XYL was more effective than ERY inhibiting 71–97% and 48–84% of isolates, respectively, depending of concentrations. 48% of clinical and all throat strains were inhibited by polyols in all concentrations (PES 3). PES was negative or zero in 26% of the isolates in the presence of ERY and in 19% of XYL. ERY enhanced the growth of S. pyogenes isolated from pus with high amylase levels. Polyols in all concentrations inhibited the growth in exponential phase. In conclusion, ERY and XYL are potent growth inhibitors of S. pyogenes isolated from PTA. Therefore, ERY and XYL may have potential in preventing PTA in the patients with frequent tonsillitis episodes.

Molecular cloning and nucleotide sequence analysis of the gene encoding the immunoreactive Brucella abortus Hsp60 protein, BA60K

1992 ◽  
Vol 12 (1) ◽  
pp. 47-62 ◽  
Author(s):  
R.Martin Roop ◽  
Michelle L. Price ◽  
Bruce E. Dunn ◽  
Stephen M. Boyle ◽  
Nammalwar Sriranganathan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Molecular Cloning ◽  
Brucella Abortus ◽  
Nucleotide Sequence Analysis ◽  
Gene Encoding ◽  
Hsp60 Protein

Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

2013 ◽  
Vol 35 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Chang-Ning Li ◽  
Manoj-Kumar Srivastava ◽  
Qian Nong ◽  
Li-Tao Yang ◽  
Yang-Rui Li
Keyword(s):  
Molecular Cloning ◽  
Saccharum Officinarum ◽  
Gene Encoding ◽  
Novel Gene
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