scholarly journals Isolation and Characterization of Human Gut Bacteria Capable of Extracellular Electron Transport by Electrochemical Techniques

2019 ◽  
Vol 9 ◽  
Author(s):  
Divya Naradasu ◽  
Waheed Miran ◽  
Mitsuo Sakamoto ◽  
Akihiro Okamoto
Keyword(s):  
Electron Transport ◽  
Electrochemical Techniques ◽  
Gut Bacteria ◽  
Human Gut ◽  
Isolation And Characterization ◽  
Extracellular Electron Transport

Isolation and Characterization of Bifidobacteria from diverse ecosystems

2019 ◽  
Vol 8 (2) ◽  
pp. 43-48
Author(s):  
Halima Aurass Bahloul ◽  
Hadadji Miloud ◽  
Mebrouk Kihal
Keyword(s):  
Newborn Infants ◽  
Food Samples ◽  
High Concentration ◽  
Dominant Group ◽  
Human Gut ◽  
Important Place ◽  
Isolation And Characterization ◽  
Probiotic Strains ◽  
Commercial Milk

Bifidobacteria represent the largest group of human intes nal bacteria. They have an important place in human health and represent the dominant group of micro ora. For this reason, they are key members of the human gut microbiota, particularly during the first one to two years following birth. The aim of this study was to test the sensibility of Bifidobacterium isolated from some strains at different concentrations of salinity and high concentration of bile as a criterion to select probiotic strains. Following the behavior of strains of Bifidobacteria isolated from different ecosystems was considered in order to develop the therapeu c aliment food . Samples from newborn infants aged 3 months, bee intestine, fermented commercial milk and yoghurt of bifidus containing Bifidobacteria was used and the isolated strains belonged to -breve and -longum species. The results of resisitance of our species of Bifidobacteria demonstrated a total inibition of croissance at 5.5% of NaCl but in 5% media of NaCl we observed a moderate croissance at di erent me of kinetics croissance .

scholarly journals An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies

2017 ◽  
Vol 33 (S1) ◽  
pp. S-31-S-39 ◽  
Author(s):  
Martin Trapecar ◽  
Shahzada Khan ◽  
Nadia R. Roan ◽  
Tsui-Hua Chen ◽  
Sushama Telwatte ◽  
...  
Keyword(s):  
Human Gut ◽  
Isolation And Characterization

Isolation and characterization of gut bacteria of fruit fly, Bactrocera tau (Walker)

2012 ◽  
Vol 41 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Chandra S. Prabhakar ◽  
Pankaj Sood ◽  
Sarbjit S. Kanwar ◽  
Prem N. Sharma ◽  
Ajay Kumar ◽  
...  
Keyword(s):  
Fruit Fly ◽  
Gut Bacteria ◽  
Isolation And Characterization

scholarly journals Characterization and Detection of a Widely Distributed Gene Cluster That Predicts Anaerobic Choline Utilization by Human Gut Bacteria

mBio ◽  
2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Ana Martínez-del Campo ◽  
Smaranda Bodea ◽  
Hilary A. Hamer ◽  
Jonathan A. Marks ◽  
Henry J. Haiser ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Gene Cluster ◽  
Gut Bacteria ◽  
Functional Gene ◽  
Metagenomic Data ◽  
Human Gut ◽  
Bacterial Gene ◽  
Human Gut Microbiota ◽  
Choline Metabolism

ABSTRACTElucidation of the molecular mechanisms underlying the human gut microbiota's effects on health and disease has been complicated by difficulties in linking metabolic functions associated with the gut community as a whole to individual microorganisms and activities. Anaerobic microbial choline metabolism, a disease-associated metabolic pathway, exemplifies this challenge, as the specific human gut microorganisms responsible for this transformation have not yet been clearly identified. In this study, we established the link between a bacterial gene cluster, the choline utilization (cut) cluster, and anaerobic choline metabolism in human gut isolates by combining transcriptional, biochemical, bioinformatic, and cultivation-based approaches. Quantitative reverse transcription-PCR analysis andin vitrobiochemical characterization of twocutgene products linked the entire cluster to growth on choline and supported a model for this pathway. Analyses of sequenced bacterial genomes revealed that thecutcluster is present in many human gut bacteria, is predictive of choline utilization in sequenced isolates, and is widely but discontinuously distributed across multiple bacterial phyla. Given that bacterial phylogeny is a poor marker for choline utilization, we were prompted to develop a degenerate PCR-based method for detecting the key functional gene choline TMA-lyase (cutC) in genomic and metagenomic DNA. Using this tool, we found that new choline-metabolizing gut isolates universally possessedcutC. We also demonstrated that this gene is widespread in stool metagenomic data sets. Overall, this work represents a crucial step toward understanding anaerobic choline metabolism in the human gut microbiota and underscores the importance of examining this microbial community from a function-oriented perspective.IMPORTANCEAnaerobic choline utilization is a bacterial metabolic activity that occurs in the human gut and is linked to multiple diseases. While bacterial genes responsible for choline fermentation (thecutgene cluster) have been recently identified, there has been no characterization of these genes in human gut isolates and microbial communities. In this work, we use multiple approaches to demonstrate that the pathway encoded by thecutgenes is present and functional in a diverse range of human gut bacteria and is also widespread in stool metagenomes. We also developed a PCR-based strategy to detect a key functional gene (cutC) involved in this pathway and applied it to characterize newly isolated choline-utilizing strains. Both our analyses of thecutgene cluster and this molecular tool will aid efforts to further understand the role of choline metabolism in the human gut microbiota and its link to disease.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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