scholarly journals Construction and Evaluation of a Korean Native Microbial Consortium for the Bioremediation of Diesel Fuel-Contaminated Soil in Korea

2018 ◽  
Vol 9 ◽  
Author(s):  
Yunho Lee ◽  
Sang Eun Jeong ◽  
Moonsuk Hur ◽  
Sunghwan Ko ◽  
Che Ok Jeon
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil ◽  
Microbial Consortium

scholarly journals Rapid Mineralization of Benzo[a]pyrene by a Microbial Consortium Growing on Diesel Fuel

2000 ◽  
Vol 66 (10) ◽  
pp. 4205-4211 ◽  
Author(s):  
Robert A. Kanaly ◽  
Richard Bartha ◽  
Kazuya Watanabe ◽  
Shigeaki Harayama
Keyword(s):  
Incubation Period ◽  
Diesel Fuel ◽  
Microbial Consortium ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Cell Protein ◽  
Rrna Gene ◽  
Gradient Gel Electrophoresis ◽  
Balance Analysis ◽  
Pyrene Concentration ◽  
Sequence Types

ABSTRACT A microbial consortium which rapidly mineralized the environmentally persistent pollutant benzo[a]pyrene was recovered from soil. The consortium cometabolically converted [7-14C]benzo[a]pyrene to14CO2 when it was grown on diesel fuel, and the extent of benzo[a]pyrene mineralization was dependent on both diesel fuel and benzo[a]pyrene concentrations. Addition of diesel fuel at concentrations ranging from 0.007 to 0.2% (wt/vol) stimulated the mineralization of 10 mg of benzo[a]pyrene per liter 33 to 65% during a 2-week incubation period. When the benzo[a]pyrene concentration was 10 to 100 mg liter−1 and the diesel fuel concentration was 0.1% (wt/vol), an inoculum containing 1 mg of cell protein per liter (small inoculum) resulted in mineralization of up to 17.2 mg of benzo[a]pyrene per liter in 16 days. This corresponded to 35% of the added radiolabel when the concentration of benzo[a]pyrene was 50 mg liter−1. A radiocarbon mass balance analysis recovered 25% of the added benzo[a]pyrene solubilized in the culture suspension prior to mineralization. Populations growing on diesel fuel most likely promoted emulsification of benzo[a]pyrene through the production of surface-active compounds. The consortium was also analyzed by PCR-denaturing gradient gel electrophoresis of 16S rRNA gene fragments, and 12 dominant bands, representing different sequence types, were detected during a 19-day incubation period. The onset of benzo[a]pyrene mineralization was compared to changes in the consortium community structure and was found to correlate with the emergence of at least four sequence types. DNA from 10 sequence types were successfully purified and sequenced, and that data revealed that eight of the consortium members were related to the classProteobacteria but that the consortium also included members which were related to the genera Mycobacterium andSphingobacterium.

Effect of activated charcoal on bioremediation of diesel fuel-contaminated soil

Microbiology ◽  
2014 ◽  
Vol 83 (5) ◽  
pp. 589-598 ◽  
Author(s):  
N. N. Semenyuk ◽  
V. S. Yatsenko ◽  
E. R. Strijakova ◽  
A. E. Filonov ◽  
K. V. Petrikov ◽  
...  
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil ◽  
Activated Charcoal

scholarly journals Assessment of diesel fuel uptake by fungi isolated from petroleum contaminated soil

2017 ◽  
pp. 221-229
Author(s):  
Zeljko Savkovic ◽  
Nevena Vukojicic ◽  
Milos Stupar ◽  
Nikola Novakovic ◽  
Nikola Unkovic ◽  
...  
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil ◽  
Growth Dynamics ◽  
Colony Growth ◽  
Dry Mass ◽  
Mass Measurements ◽  
Colony Diameter ◽  
Penicillium Sp ◽  
Production Ratio ◽  
Petroleum Contaminated Soil

The aim of this research was to isolate and identify the fungi from petroleum-contaminated soil, to test their ability to grow in the presence of diesel fuel and uptake it in solid and broth media. Among 16 isolated and identified filamentous fungi, six were tested to determine their growth on media enriched with diesel fuel as a sole carbon source. All tested isolates showed different degree of diesel fuel utilization. Colony diameter was measured, where the highest colony growth dynamics ratio was demonstrated for Penicillium sp. (74.16%) and Cladosporium sp. (71.43%), and the lowest for Absidia spinosa (23.15%). Results of dry mass measurements demonstrated the highest potential of A. strictum (71.43%) to utilize diesel fuel in broth medium. All other tested isolates showed insignificant dry mass production ratio values. The highest utilization degree was documented for A. strictum (60.28 %) and the lowest for Penicillium sp. (25.18%).

Ozonation and Fenton Treatment for Remediation of Diesel Fuel Contaminated Soil

2006 ◽  
Vol 28 (1) ◽  
pp. 37-46 ◽  
Author(s):  
A. Goi ◽  
M. Trapido ◽  
N. Kulik ◽  
M. R. T. Palmroth ◽  
T. Tuhkanen
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil
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