scholarly journals Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

2018 ◽  
Vol 9 ◽  
Author(s):  
Learn-Han Lee ◽  
Nurul-Syakima Ab Mutalib ◽  
Jodi Woan-Fei Law ◽  
Sunny Hei Wong ◽  
Vengadesh Letchumanan
Keyword(s):  
Antibiotic Resistance ◽  
Freshwater Fish ◽  
Vibrio Parahaemolyticus ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Isolation, detection of virulence genes, antibiotic resistance genes, plasmid profile, and molecular typing among Vibrio parahaemolyticus isolated in Malaysian seawater from recreational beaches and fish

2019 ◽  
Vol 12 (7) ◽  
pp. 1140-1149 ◽  
Author(s):  
Orooba Meteab Faja ◽  
Ali Abd Sharad ◽  
Khansa Mohammed Younis ◽  
Merriam Ghadhanfar Alwan ◽  
Basima Jasim Mohammed ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Vibrio Parahaemolyticus ◽  
Plasmid Profile ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
Gene Markers ◽  
Recreational Beaches ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Background and Aim: Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Materials and Methods: Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Results: Seventy-three Vibrio isolates were recovered. Only one gene tdh> from seawater isolates of Vibrio has high virulence gene percentage (95.23%). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87% and 80.64%, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100% of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. Conclusion: This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks.

Molecular characterization and antimicrobial susceptibility patterns of Clostridium difficile strains isolated from hospitals in south-east Scotland

2007 ◽  
Vol 56 (7) ◽  
pp. 921-929 ◽  
Author(s):  
Esvet Mutlu ◽  
Allison J. Wroe ◽  
Karla Sanchez-Hurtado ◽  
Jon S. Brazier ◽  
Ian R. Poxton
Keyword(s):  
Antibiotic Resistance ◽  
Clostridium Difficile ◽  
Restriction Analysis ◽  
Common Type ◽  
Resistance Pattern ◽  
Binary Toxin ◽  
Resistance Patterns ◽  
The Common ◽  
Antibiotic Resistance Patterns ◽  
Susceptibility Patterns

Clostridium difficile isolates (n=149) collected in south-east Scotland between August and October 2005 were typed by four different methods and their susceptibility to seven different antibiotics was determined. The aims were to define the types of strain occurring in this region and to determine whether there were any clonal relationships among them with respect to genotype and antibiotic resistance pattern. Ribotyping revealed that 001 was the most common type (n=113, 75.8 %), followed by ribotype 106 (12 isolates, 8.1 %). The majority of the isolates (96.6 %, n=144) were of toxinotype 0, with two toxinotype V isolates and single isolates of toxinotypes I, IV and XIII. PCR and restriction analysis of the fliC gene from 147 isolates gave two restriction patterns: 145 of pattern VII and two of pattern I. Binary toxin genes were detected in only three isolates: two isolates of ribotype 126, toxinotype V, and one isolate of ribotype 023, toxinotype IV. S-types showed more variation, with 64.5 % (n=40) of the common S-type (4939) and 21 % (n=13) of S-type 4741, with six other S-types (one to three isolates each). All ribotype 001 isolates were of the same S-type (4939), with three isolates of other ribotypes being this S-type. No resistance was found to metronidazole or vancomycin, with resistance to tetracycline only found in 4.3 % of the isolates. A high proportion of isolates were resistant to clindamycin (62.9 %), moxifloxacin, ceftriaxone (both 87.1 %) and erythromycin (94.8 %). Resistance to three antibiotics (erythromycin, clindamycin and ceftriaxone) was seen in 66 isolates, with erythromycin, ceftriaxone and moxifloxacin resistance seen in 96 isolates. Resistance to all four of these antibiotics was found in 62 isolates and resistance to five (the above plus tetracycline) in one isolate: a ribotype 001, toxinotype 0 strain. Whilst ribotype 001 was the most commonly encountered type, there was no evidence of clonal relationships when all other typing and antibiotic resistance patterns were taken into account.

Antibiotic resistance patterns of Acinetobacter calcoaceticus–A. baumannii complex species from Colombian hospitals

2013 ◽  
Vol 31 (3) ◽  
pp. 142-146 ◽  
Author(s):  
María Teresa Reguero ◽  
Olga Esther Medina ◽  
María Andrea Hernández ◽  
Diana Vanessa Flórez ◽  
Emilia María Valenzuela ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Calcoaceticus ◽  
Complex Species ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns
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