scholarly journals Characterization of Two Trichinella spiralis Adult-Specific DNase II and Their Capacity to Induce Protective Immunity

2018 ◽  
Vol 9 ◽  
Author(s):  
Xin Qi ◽  
Xin Yue ◽  
Yue Han ◽  
Peng Jiang ◽  
Fan Yang ◽  
...  
Keyword(s):  
Protective Immunity ◽  
Trichinella Spiralis ◽  
Dnase Ii

Trichinella spiralis: Characterization of phage-displayed specific epitopes and their protective immunity in BALB/c mice

2008 ◽  
Vol 118 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Yuan Gu ◽  
Jie Li ◽  
Xinping Zhu ◽  
Jing Yang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Protective Immunity ◽  
Trichinella Spiralis

Immunochemical Characterization of Setaria cervi Microfilarial Antigens Using Novel Antibodies

2019 ◽  
Vol 19 (14) ◽  
pp. 1263-1274 ◽  
Author(s):  
Anuradha Kalani ◽  
Komal Kalani ◽  
Poonam Chaturvedi ◽  
Pankaj Chaturvedi
Keyword(s):  
Adult Worm ◽  
Diagnostic Tests ◽  
Protective Immunity ◽  
Complex Nature ◽  
Immune Recognition ◽  
Setaria Cervi ◽  
Crossed Immunoelectrophoresis ◽  
Study Results ◽  
Immune Rabbit

Background:Filariasis affects millions of people in tropical and subtropical regions of the world and is caused by nematode roundworm. In order to develop a vaccine and specific diagnostic tests, it is important to characterize different stages of the filarial worms. Microfilariae (Mf) stage of the roundworm is found in host’s blood or lymph vessels and can be important not only for developing better immunodiagnostics but also for understanding immune recognition and its relevance to immunepathogenesis and protective immunity.Objective:The present study aimed to immunocharacterize Mf and adult worm antigens that could be helpful in future diagnostic tests.Method:Four different immune sera against Setaria cervi intact live, intact live with adjuvant, intact glutaraldehyde fixed with adjuvant and total somatic Mf were prepared and used for the immunocharacterization of Mf antigens.Results:Our study results suggest that compared to fixed intact Mf, live intact Mf are more immunogenic, as the immune sera generated against intact live Mf showed high ELISA reactivity with Setaria cervi Mf and adult worm antigens. All the four immune sera IgG fractions had surface specificity as determined through considerable ELISA reactivity with S. cervi intact Mf. When tested under native conditions (immunoelectrophoresis and crossed immunoelectrophoresis), all the four immune rabbit sera were able to detect antigens of S. cervi Mf and adult stages.Conclusion:These results can be useful in detailed understanding of the complex nature of the Mf and adult antigens, which are prerequisites in the development of vaccine and more specific diagnostic tests.

Workshop on a detailed characterization of Trichinella spiralis antigens: a platform for future studies on antigens and antibodies to this parasite

1996 ◽  
Vol 18 (6) ◽  
pp. 273-284 ◽  
Author(s):  
M.G. ORTEGA-PIERRES ◽  
L. YEPEZ-MULIA ◽  
W. HOMAN ◽  
H.R .GAMBLE ◽  
P.L. LIM ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Detailed Characterization ◽  
Future Studies

Oral vaccination with attenuated Salmonella encoding the Trichinella spiralis 43-kDa protein elicits protective immunity in BALB/c mice

Acta Tropica ◽  
2021 ◽  
pp. 106071
Author(s):  
Nan Wang ◽  
Jun-Yao Wang ◽  
Yan-Long Jiang ◽  
Hai-Bin Huang ◽  
Wen-Tao Yang ◽  
...  
Keyword(s):  
Protective Immunity ◽  
Trichinella Spiralis ◽  
Oral Vaccination

Characterization of TsDAF-21/HSP90 protein from the parasitic nematode Trichinella spiralis

2014 ◽  
Vol 113 (6) ◽  
pp. 2209-2217 ◽  
Author(s):  
Yurong Yang ◽  
Weiwen Qin ◽  
Hengtong Qiu ◽  
Yan Liu
Keyword(s):  
Parasitic Nematode ◽  
Trichinella Spiralis ◽  
Hsp90 Protein

scholarly journals Cellular and molecular changes and immune response in the intestinal mucosa during Trichinella spiralis early infection in rats

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
María Priscila Saracino ◽  
Cecilia Celeste Vila ◽  
Melina Cohen ◽  
María Virginia Gentilini ◽  
Guido Hernán Falduto ◽  
...  
Keyword(s):  
Immune Response ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Mucosal Immune Response ◽  
Mesenteric Lymph Nodes ◽  
Muscle Larvae ◽  
Gut Immunity ◽  
Gut Mucosa

Abstract Background: The main targets of the host’s immune system in Trichinella spiralis infection are the adult worms (AW), at the gut level, and the migrant or newborn larvae (NBL), at systemic and pulmonary levels. Most of the studies carried out in the gut mucosa have been performed on the Payer’s patches and/or the mesenteric lymph nodes but not on the lamina propria, therefore, knowledge on the gut immune response against T. spiralis remains incomplete. Methods This study aimed at characterizing the early mucosal immune response against T. spiralis, particularly, the events taking place between 1 and 13 dpi. For this purpose, Wistar rats were orally infected with muscle larvae of T. spiralis and the humoral and cellular parameters of the gut immunity were analysed, including the evaluation of the ADCC mechanism exerted by lamina propria cells. Results A marked inflammation and structural alteration of the mucosa was found. The changes involved an increase in goblet cells, eosinophils and mast cells, and B and T lymphocytes, initially displaying a Th1 profile, characterised by the secretion of IFN-γ and IL-12, followed by a polarization towards a Th2 profile, with a marked increase in IgE, IgG1, IL-4, IL-5 and IL-13 levels, which occurred once the infection was established. In addition, the helminthotoxic activity of lamina propria cells demonstrated the role of the intestine as a place of migrant larvae destruction, indicating that not all the NBLs released in the gut will be able to reach the muscles. Conclusions The characterization of the immune response triggered in the gut mucosa during T. spiralis infection showed that not only an effector mechanism is directed toward the AW but also towards the NBL as a cytotoxic activity was observed against NBL exerted by lamina propria cells.

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