scholarly journals Transcription Factor SsSte12 Was Involved in Mycelium Growth and Development in Sclerotinia sclerotiorum

2018 ◽  
Vol 9 ◽  
Author(s):  
Tingtao Xu ◽  
Jingtao Li ◽  
Baodong Yu ◽  
Ling Liu ◽  
Xianghui Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Sclerotinia Sclerotiorum ◽  
Growth And Development ◽  
Mycelium Growth

scholarly journals Redox-Responsive Transcription Factor 1 (RRFT1) Is Involved in Extracellular ATP-Regulated Arabidopsis thaliana Seedling Growth

2020 ◽  
Vol 61 (4) ◽  
pp. 685-698 ◽  
Author(s):  
Ruojia Zhu ◽  
Xiaoxia Dong ◽  
Yingying Xue ◽  
Jiawei Xu ◽  
Aiqi Zhang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Adenosine Triphosphate ◽  
Seedling Growth ◽  
Growth And Development ◽  
High Throughput Sequencing ◽  
Root Tip ◽  
Loss Of Function ◽  
Redox Responsive ◽  
Transcription Factor 1

Abstract Extracellular adenosine triphosphate (eATP) is an apoplastic signaling molecule that plays an essential role in the growth and development of plants. Arabidopsis seedlings have been reported to respond to eATP; however, the downstream signaling components are still not well understood. In this study, we report that an ethylene-responsive factor, Redox-Responsive Transcription Factor 1 (RRTF1), is involved in eATP-regulated Arabidopsis thaliana seedling growth. Exogenous adenosine triphosphate inhibited green seedling root growth and induced hypocotyl bending of etiolated seedlings. RRTF1 loss-of-function mutant (rrtf1) seedlings showed decreased responses to eATP, while its complementation or overexpression led to recovered or increased eATP responsiveness. RRTF1 was expressed rapidly after eATP stimulation and then migrated into the nuclei of root tip cells. eATP-induced auxin accumulation in root tip or hypocotyl cells was impaired in rrtf1. Chromatin immunoprecipitation and high-throughput sequencing results indicated that eATP induced some genes related to cell growth and development in wild type but not in rrtf1 cells. These results suggest that RRTF1 may be involved in eATP signaling by regulating functional gene expression and cell metabolism in Arabidopsis seedlings.

scholarly journals The role of gum induced by Fusarium oxysporum Schlecht. Snyd. et Hans. f.sp. tulipae Apt. in tulip bulbs on growth and development of the pathogen

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 432-435
Author(s):  
A. Saniewska
Keyword(s):  
Fusarium Oxysporum ◽  
Growth And Development ◽  
Mycelium Growth ◽  
Tulip Bulbs ◽  
Bacto Agar

It was showed that gums induced by Fusarium oxysporum f.sp. tulipae in tulip bulbs applied to mineral Czapek-Dox Broth with Bacto Agar (CzDA) medium, containing sucrose substantially stimulated abundant growth of mycelium and sporulation of the pathogen. Addition of arabinose, xylose and their mixture, sugars occurring in tulip gum polysaccharide, to CzDA medium caused that mycelium was very sparse and sporulation was only slightly stimulated by arabinose. Mycelium growth on mineral CzDA medium without sucrose, was poor and sparse but addition of gum to the medium caused formation of abundant mycelium and increased sporulation of the pathogen. It is possible that polysaccharide of tulip gum may act mainly as elicitor and partially as substrate in regulation of mycelium growth and sporulation of Fusarium oxysporum f.sp. tulipae.

scholarly journals The Formaldehyde Dehydrogenase SsFdh1 Is Regulated by and Functionally Cooperates with the GATA Transcription Factor SsNsd1 in Sclerotinia sclerotiorum

mSystems ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Genglin Zhu ◽  
Gang Yu ◽  
Xianghui Zhang ◽  
Jinliang Liu ◽  
Yanhua Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Sclerotinia Sclerotiorum ◽  
Promoter Region ◽  
Nuclear Translocation ◽  
Effective Control ◽  
Formaldehyde Dehydrogenase ◽  
Content Type ◽  
Gata Transcription Factor ◽  
Gata Box

ABSTRACT GATA transcription factors (TFs) are common eukaryotic regulators, and glutathione-dependent formaldehyde dehydrogenases (GD-FDH) are ubiquitous enzymes with formaldehyde detoxification activity. In this study, the formaldehyde dehydrogenase Sclerotinia sclerotiorum Fdh1 (SsFdh1) was first characterized as an interacting partner of a GATA TF, SsNsd1, in S. sclerotiorum. Genetic analysis reveals that SsFdh1 functions in formaldehyde detoxification, nitrogen metabolism, sclerotium development, and pathogenicity. Both SsNsd1 and SsFdh1 harbor typical zinc finger motifs with conserved cysteine residues. SsNsd1 regulates SsFdh1 in two distinct manners. SsNsd1 directly binds to GATA-box DNA in the promoter region of Ssfdh1; SsNsd1 associates with SsFdh1 through disulfide bonds formed by conserved Cys residues. The SsNsd1-SsFdh1 interaction and nuclear translocation were found to prevent efficient binding of SsNsd1 to GATA-box DNA. Site-directed point mutation of these Cys residues influences the SsNsd1-SsFdh1 interaction and SsNsd1 DNA binding capacity. SsFdh1 is regulated by and functions jointly with the SsNsd1 factor, providing new insights into the complex transcriptional regulatory mechanisms of GATA factors. IMPORTANCE S. sclerotiorum is a pathogenic fungus with sclerotium and infection cushion development, making S. sclerotiorum one of the most challenging agricultural pathogens with no effective control method. We identified important sclerotium and compound appressorium formation determinants, SsNsd1 and SsFdh1, and investigated their regulatory mechanism at the molecular level. SsNsd1 and SsFdh1 are zinc finger motif-containing proteins and associate with each other in the nucleus. On other hand, SsNsd1, as a GATA transcription factor, directly binds to GATA-box DNA in the promoter region of Ssfdh1. The SsNsd1-SsFdh1 interaction and nuclear translocation were found to prevent efficient binding of SsNsd1 to GATA-box DNA. Our results provide insights into the role of the GATA transcription factor and its regulation of formaldehyde dehydrogenase in stress resistance, fungal sclerotium and compound appressorium development, and pathogenicity.

scholarly journals Genome-Wide Identification and Expression Analyses of the bZIP Transcription Factor Genes in moso bamboo (Phyllostachys edulis)

2019 ◽  
Vol 20 (9) ◽  
pp. 2203 ◽  
Author(s):  
Feng Pan ◽  
Min Wu ◽  
Wenfang Hu ◽  
Rui Liu ◽  
Hanwei Yan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Growth And Development ◽  
Expression Profiles ◽  
Forest Products ◽  
Regulatory Elements ◽  
Moso Bamboo ◽  
Bzip Transcription Factor ◽  
Phyllostachys Edulis

The basic leucine zipper (bZIP) transcription factor (TF) family is one of the largest gene families, and play crucial roles in many processes, including stress responses, hormone effects. The TF family also participates in plant growth and development. However, limited information is available for these genes in moso bamboo (Phyllostachys edulis), one of the most important non-timber forest products in the world. In the present study, 154 putative PhebZIP genes were identified in the moso bamboo genome. The phylogenetic analyses indicate that the PhebZIP gene proteins classify into 9 subfamilies and the gene structures and conserved motifs that analyses identified among all PhebZIP proteins suggested a high group-specificity. Microsynteny and evolutionary patterns analyses of the non-synonymous (Ka) and synonymous (Ks) substitution rates and their ratios indicated that paralogous pairs of PhebZIP genes in moso bamboo underwent a large-scale genome duplication event that occurred 7–15 million years ago (MYA). According to promoter sequence analysis, we further selected 18 genes which contain the higher number of cis-regulatory elements for expression analysis. The result showed that these genes are extensively involved in GA-, ABA- and MeJA-responses, with possibly different mechanisms. The tissue-specific expression profiles of PhebZIP genes in five plant tissues/organs/developmental stages suggested that these genes are involved in moso bamboo organ development, especially seed development. Subcellular localization and transactivation activity analysis showed that PhebZIP47 and PhebZIP126 were localized in the nucleus and PhebZIP47 with no transcriptional activation in yeast. Our research provides a comprehensive understanding of PhebZIP genes and may aid in the selection of appropriate candidate genes for further cloning and functional analysis in moso bamboo growth and development, and improve their resistance to stress during their life.

A putative APSES transcription factor is necessary for normal growth and development of Aspergillus nidulans

2013 ◽  
Vol 51 (6) ◽  
pp. 800-806 ◽  
Author(s):  
Ji-Yeon Lee ◽  
Lee-Han Kim ◽  
Ha-Eun Kim ◽  
Jae-Sin Park ◽  
Kap-Hoon Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Aspergillus Nidulans ◽  
Growth And Development ◽  
Normal Growth

scholarly journals pH Signaling in Sclerotinia sclerotiorum: Identification of a pacC/RIM1 Homolog

2001 ◽  
Vol 67 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Jeffrey A. Rollins ◽  
Martin B. Dickman
Keyword(s):  
Transcription Factor ◽  
Oxalic Acid ◽  
Signaling Pathway ◽  
Zinc Finger ◽  
Sclerotinia Sclerotiorum ◽  
Culture Conditions ◽  
Phytopathogenic Fungi ◽  
Molecular Signaling ◽  
Ph Conditions ◽  
Zinc Finger Domains

ABSTRACT Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotiorum. Exposure to increasing alkaline ambient pH increased the oxalic acid accumulation independent of carbon source, sclerotial development was favored by acidic ambient pH conditions but inhibited by neutral ambient pH, and transcripts encoding the endopolygalacturonase gene pg1 accumulated maximally under acidic culture conditions. We cloned a putative transcription factor-encoding gene, pac1, that may participate in a molecular signaling pathway for regulating gene expression in response to ambient pH. The three zinc finger domains of the predicted Pac1 protein are similar in sequence and organization to the zinc finger domains of the A. nidulans pH-responsive transcription factor PacC. The promoter of pac1 contains eight PacC consensus binding sites, suggesting that this gene, like its homologs, is autoregulated. Consistent with this suggestion, the accumulation ofpac1 transcripts paralleled increases in ambient pH. Pac1 was determined to be a functional homolog of PacC by complementation of an A. nidulans pacC-null strain with pac1. Our results suggest that ambient pH is a regulatory cue for processes linked to pathogenicity, development, and virulence and that these processes may be under the molecular regulation of a conserved pH-dependent signaling pathway analogous to that in the nonpathogenic fungus A. nidulans.

scholarly journals Molecular Mechanisms Governing Shade Responses in Maize

2018 ◽  
Author(s):  
Qingbiao Shi ◽  
Fanying Kong ◽  
Haisen Zhang ◽  
Yu’e Jiang ◽  
Siqi Heng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Regulatory Network ◽  
Growth And Development ◽  
Molecular Mechanisms ◽  
Enrichment Analysis ◽  
Shade Avoidance ◽  
Factors Affecting ◽  
Transcriptional Dynamics ◽  
Enhanced Expression ◽  
Maize Genetics

AbstractLight is one of the most important environmental factors affecting plant growth and development. Plants use shade avoidance and shade tolerance strategies to adjust their growth and development thus increase their success in the competition for incoming light. To investigate the mechanism of shade responses in maize (Zea mays), we examined the anatomical and transcriptional dynamics of the early shade response in seedlings of the B73 inbred line. Transcriptome analysis identified 912 differentially expressed genes, including genes involved in light signaling, auxin responses, and cell elongation pathways. Grouping transcription factor family genes and performing enrichment analysis identified multiple types of transcription factors that are differentially regulated by shade and predicted putative core genes responsible for regulating shade avoidance syndrome. For functional tests, we ectopically over-expressed ZmHB53, a type II HD-ZIP transcription factor gene significantly induced by shade, in Arabidopsis thaliana. Transgenic Arabidopsis plants overexpressing ZmHB53 exhibited narrower leaves, earlier flowering, and enhanced expression of shade-responsive genes, suggesting that ZmHB53 participates in the regulation of shade responses in maize. This study increases our understanding of the regulatory network of the shade response in maize and provides a useful resource for maize genetics and breeding.HighlightOur findings not only increase the understanding of the regulatory network of the shade avoidance in maize, and also provide a useful resource for maize genetics and breeding.

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