scholarly journals Therapeutic Targets in Chlamydial Fatty Acid and Phospholipid Synthesis

2018 ◽  
Vol 9 ◽  
Author(s):  
Jiangwei Yao ◽  
Charles O. Rock
Keyword(s):  
Fatty Acid ◽  
Therapeutic Targets ◽  
Phospholipid Synthesis

scholarly journals Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion during Autophagy

Cell ◽  
2020 ◽  
Vol 180 (1) ◽  
pp. 135-149.e14 ◽  
Author(s):  
Maximilian Schütter ◽  
Patrick Giavalisco ◽  
Susanne Brodesser ◽  
Martin Graef
Keyword(s):  
Fatty Acid ◽  
Phospholipid Synthesis

Regulation of ethanolamine and choline phosphatide biosynthesis in isolated rat intestinal villus cells

1980 ◽  
Vol 58 (7) ◽  
pp. 527-533 ◽  
Author(s):  
P. J. A. O'Doherty
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lauric Acid ◽  
Unsaturated Fatty Acids ◽  
Phospholipid Synthesis ◽  
Intestinal Villus ◽  
Maximal Stimulation ◽  
Rate Limiting ◽  
Isolated Rat

The effects of ethanolamine, choline, and different fatty acids on phospholipid synthesis via the CDP-ester pathways were studied in isolated rat intestinal villus cells. The incorporation of [14C]glucose into phosphatidylethanolamine was stimulated severalfold by the addition of ethanolamine and long-chained unsaturated fatty acids, while the addition of lauric acid inhibited the incorporation of radioactivity into phosphatidylethanolamine. At concentrations of ethanolamine higher than 0.2 mM, phosphoethanolamine accumulated, but the concentration of CDP-ethanolamine and the incorporation of radioactivity into phosphatidylethanolamine did not increase further. The incorporation of [14C]glucose into phosphatidylcholine responded in a way similar to that of phosphatidylethanolamine, except that a 10-fold higher concentration of choline was required for maximal stimulation. CCC inhibited the incorporation of choline into phosphatidylcholine. In contrast with hepatocytes, villus cells did not form phosphatidylcholine via phospholipid N-methylation. The data indicate that, in intestinal villus cells, the cytidylyltransferase reactions are rate limiting in the synthesis of phosphatidylethanolamine and probably also of phosphatidylcholine. The availability of diacylglycerol and its fatty acid composition may also significantly affect the rate of phospholipid synthesis.

Induction of fatty acid and phospholipid synthesis is a key requirement for phagocytic differentiation of human monocytes

2010 ◽  
Vol 163 ◽  
pp. S29
Author(s):  
Josef Ecker ◽  
Gerhard Liebisch ◽  
Marion Englmaier ◽  
Margot Grandl ◽  
Horst Robenek ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Human Monocytes ◽  
Phospholipid Synthesis

scholarly journals On the tight coupling of phospholipid synthesis and fatty acid desaturation in Chlorella vulgaris

1968 ◽  
Vol 110 (4) ◽  
pp. 49P-50P ◽  
Author(s):  
M I Gurr ◽  
M P Robinson ◽  
R W Sword ◽  
A T James
Keyword(s):  
Fatty Acid ◽  
Chlorella Vulgaris ◽  
Fatty Acid Desaturation ◽  
Phospholipid Synthesis ◽  
Tight Coupling

Biosynthetic utilization of photoreactive fatty acids by rat liver microsomes

1984 ◽  
Vol 62 (6) ◽  
pp. 375-378 ◽  
Author(s):  
Pierre Leblanc ◽  
Gerhard E. Gerber
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rat Liver ◽  
Alkyl Chain ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Phospholipid Synthesis ◽  
Derivatives Of

The photoreactive ω-diazirinophenoxy derivatives of nonanoate, undecanoate, tridecanoate, and pentadecanoate were shown to be activated by rat liver microsomes to the corresponding acyl-CoA derivatives. The Km and Vmax for these fatty acid analogues were determined; the values obtained indicate that the addition of a photoreactive group to an alkyl chain has an effect similar to that of elongation of the chain by about seven carbons. Incubation of microsomes in the presence of lysophospholipids resulted in the incorporation of the photoreactive fatty acids into the corresponding phospholipids. The ability of mammalian systems to utilize these photoreactive fatty acids for phospholipid synthesis establishes their suitability as photoaffinity analogues of fatty acids.

scholarly journals Reconstruction of phospholipid synthesis by combing in vitro fatty acid synthesis and cell-free gene expression

2021 ◽  
Author(s):  
Sumie Eto ◽  
Rumie Matsumura ◽  
Mai Fujimi ◽  
Yasuhiro Shimane ◽  
Samuel Berhanu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Phospholipid Synthesis ◽  
Giant Vesicles ◽  
Phosphatidic Acids ◽  
A Cell ◽  
Free Gene

Phospholipid synthesis is a fundamental process that promotes cell propagation and, presently, is the most challenging issue in artificial cell research aimed at reconstituting living cells from biomolecules. Here, we constructed a cell-free phospholipid synthesis system that combines in vitro fatty acid synthesis and a cell-free gene expression system that synthesizes acyltransferases for phospholipid synthesis. Fatty acids were synthesized from acetyl-CoA and malonyl-CoA, then continuously converted into phosphatidic acids by the cell-free synthesized acyltransferases. Because the system can avoid the accumulation of synthetic intermediates that suppress the reaction, the yield of phospholipid has significantly improved from previous schemes (up to 400 μM). Additionally, by adding enzymes for recycling CoA, we synthesized phosphatidic acids from acetic acid and bicarbonate as carbon sources. The constructed system is available to express the genes from pathogenic bacteria and to analyze the synthesized phospholipids. By encapsulating our system inside giant vesicles, it would be possible to construct the artificial cells in which the membrane grows and divides sustainably.

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