scholarly journals RNA Interference as a Prospective Tool for the Control of Human Viral Infections

2018 ◽  
Vol 9 ◽  
Author(s):  
Alesia Levanova ◽  
Minna M. Poranen
Keyword(s):  
Rna Interference ◽  
Viral Infections

scholarly journals RNA Interference for Viral Infections

2012 ◽  
Vol 13 (11) ◽  
pp. 1411-1420 ◽  
Author(s):  
Stephen J. Blake ◽  
Fawzi F. Bokhari ◽  
Nigel A.J. McMillan
Keyword(s):  
Rna Interference ◽  
Viral Infections

scholarly journals Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber

2021 ◽  
Vol 22 (22) ◽  
pp. 12237
Author(s):  
Shuo Miao ◽  
Chaoqiong Liang ◽  
Jianqiang Li ◽  
Barbara Baker ◽  
Laixin Luo
Keyword(s):  
Rna Interference ◽  
Mosaic Virus ◽  
Viral Infections ◽  
Seed Transmission ◽  
Artificial Microrna ◽  
Virulent Strains ◽  
Environmentally Safe ◽  
Different Strains ◽  
Interfering Rna ◽  
Fine Tune

Cucumber green mottle mosaic virus (CGMMV), as a typical seed-borne virus, causes costly and devastating diseases in the vegetable trade worldwide. Genetic sources for resistance to CGMMV in cucurbits are limited, and environmentally safe approaches for curbing the accumulation and spread of seed-transmitted viruses and cultivating completely resistant plants are needed. Here, we describe the design and application of RNA interference-based technologies, containing artificial microRNA (amiRNA) and synthetic trans-acting small interfering RNA (syn-tasiRNA), against conserved regions of different strains of the CGMMV genome. We used a rapid transient sensor system to identify effective anti-CGMMV amiRNAs. A virus seed transmission assay was developed, showing that the externally added polycistronic amiRNA and syn-tasiRNA can successfully block the accumulation of CGMMV in cucumber, but different virulent strains exhibited distinct influences on the expression of amiRNA due to the activity of the RNA-silencing suppressor. We also established stable transgenic cucumber plants expressing polycistronic amiRNA, which conferred disease resistance against CGMMV, and no sequence mutation was observed in CGMMV. This study demonstrates that RNA interference-based technologies can effectively prevent the occurrence and accumulation of CGMMV. The results provide a basis to establish and fine-tune approaches to prevent and treat seed-based transmission viral infections.

scholarly journals Antiviral RNA Interference Activity in Cells of the Predatory Mosquito, Toxorhynchites amboinensis

Viruses ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 694 ◽  
Author(s):  
Claire Donald ◽  
Margus Varjak ◽  
Eric Aguiar ◽  
João Marques ◽  
Vattipally Sreenu ◽  
...  
Keyword(s):  
Biological Control ◽  
Rna Interference ◽  
Small Rnas ◽  
Semliki Forest Virus ◽  
Viral Infections ◽  
Biological Control Agent ◽  
Mosquito Species ◽  
Similar Species ◽  
Vector Mosquito ◽  
Rnai Response

Arthropod vectors control the replication of arboviruses through their innate antiviral immune responses. In particular, the RNA interference (RNAi) pathways are of notable significance for the control of viral infections. Although much has been done to understand the role of RNAi in vector populations, little is known about its importance in non-vector mosquito species. In this study, we investigated the presence of an RNAi response in Toxorhynchites amboinensis, which is a non-blood feeding species proposed as a biological control agent against pest mosquitoes. Using a derived cell line (TRA-171), we demonstrate that these mosquitoes possess a functional RNAi response that is active against a mosquito-borne alphavirus, Semliki Forest virus. As observed in vector mosquito species, small RNAs are produced that target viral sequences. The size and characteristics of these small RNAs indicate that both the siRNA and piRNA pathways are induced in response to infection. Taken together, this data suggests that Tx. amboinensis are able to control viral infections in a similar way to natural arbovirus vector mosquito species. Understanding their ability to manage arboviral infections will be advantageous when assessing these and similar species as biological control agents.

vhfRNAi: a web-platform for analysis of host genes involved in viral infections discovered by genome wide RNAi screens

2017 ◽  
Vol 13 (7) ◽  
pp. 1377-1387 ◽  
Author(s):  
Anamika Thakur ◽  
Abid Qureshi ◽  
Manoj Kumar
Keyword(s):  
Rna Interference ◽  
High Throughput ◽  
Viral Infections ◽  
Host Factors ◽  
Genome Wide ◽  
Host Virus Interactions ◽  
Virus Interactions ◽  
Web Platform ◽  
Host Genes

Knockdown of host genes using high-throughput genome-wide RNA interference screens has identified numerous host factors that affect viral infections, which would be helpful in understanding host–virus interactions.

Exploring the associations of host genes for viral infection revealed by genome-wide RNAi and virus–host protein interactions

2015 ◽  
Vol 11 (9) ◽  
pp. 2511-2519 ◽  
Author(s):  
Dafei Xie ◽  
Lu Han ◽  
Yifu Luo ◽  
Yang Liu ◽  
Song He ◽  
...  
Keyword(s):  
Rna Interference ◽  
Viral Infection ◽  
Cell Viability ◽  
Virus Replication ◽  
Protein Interactions ◽  
Viral Infections ◽  
Host Factors ◽  
Host Protein ◽  
Genome Wide ◽  
Host Genes

Genome-wide RNA interference screens have greatly facilitated the identification of essential host factors (EHFs) for viral infections, whose knockdown effects significantly influence virus replication but not host cell viability.

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