scholarly journals Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of H7N9 Influenza Viruses

2018 ◽  
Vol 9 ◽  
Author(s):  
Zhihao Sun ◽  
Baolan Shi ◽  
Feifei Meng ◽  
Ruonan Ma ◽  
Qingyun Hu ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza Viruses ◽  
Immunochromatographic Strip ◽  
H7n9 Influenza

Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Disease Virus ◽  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza A ◽  
Field Investigation ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Immunochromatographic Strip ◽  
Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

scholarly journals Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein

2021 ◽  
Vol 12 ◽  
Author(s):  
Ge Li ◽  
Aiping Wang ◽  
Yumei Chen ◽  
Yaning Sun ◽  
Yongkun Du ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Colloidal Gold ◽  
Global Economy ◽  
Chinese Hamster ◽  
Influenza Viruses ◽  
Spike Protein ◽  
Disease Spread ◽  
Ovary Cell ◽  
Immunochromatographic Strip ◽  
Specific Reactions

The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. The strip is highly specific, detecting only SARS-CoV-2 spike protein, and does not show any non-specific reactions with syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and other coronavirus and influenza viruses. The strip detected subunit vaccine in our laboratory with a detection limit of spike protein of 62.5 ng/mL. This strip provides an effective method in monitoring vaccine quality by detecting the antigen content of spike protein.

A colloidal gold immunochromatographic strip assay for the rapid detection of Shigella in milk and meat products

2021 ◽  
Author(s):  
beijun ang ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Bacillary Dysentery ◽  
Foodborne Pathogen ◽  
Meat Products ◽  
Immunochromatographic Strip ◽  
Large Sample ◽  
Colloidal Gold Immunochromatographic Strip

Shigella is the most common foodborne pathogen and can cause bacillary dysentery. Therefore, it is necessary to detect Shigella rapidly, accurately and to process large sample numbers, but it is...

scholarly journals Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

2020 ◽  
Vol 99 (10) ◽  
pp. 4741-4749
Author(s):  
Wenlong Han ◽  
Zongchao Chen ◽  
Pengfei Niu ◽  
Xiaomei Ren ◽  
Chan Ding ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Immunochromatographic Strip ◽  
Riemerella Anatipestifer ◽  
Colloidal Gold Immunochromatographic Strip

scholarly journals Development of an Immunochromatographic Strip for Rapid Detection of H9 Subtype Avian Influenza Viruses

2008 ◽  
Vol 15 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Fuhu Peng ◽  
Zheng Wang ◽  
Shuhui Zhang ◽  
Renwei Wu ◽  
Sishun Hu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Rapid Detection ◽  
Clinical Symptoms ◽  
High Specificity ◽  
Cloacal Swab ◽  
Influenza Viruses ◽  
Control Line ◽  
Avian Influenza Viruses ◽  
Immunochromatographic Strip

ABSTRACT An immunochromatographic strip was developed for the detection of the H9 subtype of avian influenza viruses (H9AIVs) in poultry, using two monoclonal antibodies (MAb), 4C4 for H9AIV hemagglutinin (HA) and 4D4 for nucleoprotein. The 4C4 MAb was labeled with colloidal gold as the detection reagent, and the 4D4 MAb was blotted on the test line while a goat anti-mouse antibody was used on the control line of the nitrocellulose membrane. In comparison with the HA and HA inhibition (HI) tests, the strip was specific for the detection of H9AIV, with a sensitivity at 0.25 HA units within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity and specificity. Evaluation of the strip with experimental tracheal and cloacal swab samples collected from H9N2-infected chickens revealed that the strip detected the H9N2 viruses on day 3 postinoculation, earlier than the appearance of clinical symptoms. Application of the strip for the analysis of 157 tracheal or cloacal samples from potentially infected chickens on five poultry farms showed that four farms had chickens that were infected with H9AIV. Further characterization of 10 positive and 30 negative randomly selected samples showed that no single sample was false positive or negative, as determined by the standard virus isolation and HI assays. Therefore, the immunochromatographic strip for the detection of H9AIVs has high specificity, sensitivity, and stability. This finding, together with the advantages of rapid detection and easy operation and without the requirement for special skills and equipment, makes the strip suitable for onsite detection and the differentiation of H9AIVs from other viruses in poultry.

Colloidal gold-based immunochromatographic strip assay for the rapid detection of bacitracin zinc in milk

2020 ◽  
Vol 327 ◽  
pp. 126879 ◽  
Author(s):  
Guanqiong Na ◽  
Xiaofei Hu ◽  
Jifei Yang ◽  
Yaning Sun ◽  
Sharon Kwee ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Immunochromatographic Strip

Development of a Colloidal Gold Immunochromatographic Strip Assay for Rapid Detection of Bovine Rotavirus

2019 ◽  
Vol 32 (9) ◽  
pp. 393-401
Author(s):  
Zhenxue Li ◽  
Feipeng Zhao ◽  
Tingting Tang ◽  
Mengmeng Wang ◽  
Xiaoli Yu ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Immunochromatographic Strip ◽  
Bovine Rotavirus ◽  
Colloidal Gold Immunochromatographic Strip

Colloidal gold-based immunochromatographic strip assay for the rapid detection of diminazene in milk

2020 ◽  
Vol 37 (10) ◽  
pp. 1667-1677
Author(s):  
Linlin Chen ◽  
Yaning Sun ◽  
Xiaofei Hu ◽  
Yunrui Xing ◽  
Sharon Kwee ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Immunochromatographic Strip
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