scholarly journals Single-Cell Genomics Reveals a Diverse Metabolic Potential of Uncultivated Desulfatiglans-Related Deltaproteobacteria Widely Distributed in Marine Sediment

2018 ◽  
Vol 9 ◽  
Author(s):  
Lara M. Jochum ◽  
Lars Schreiber ◽  
Ian P. G. Marshall ◽  
Bo B. Jørgensen ◽  
Andreas Schramm ◽  
...  
Keyword(s):  
Single Cell ◽  
Marine Sediment ◽  
Metabolic Potential ◽  
Single Cell Genomics

scholarly journals Harnessing the Genetic Diversity and Metabolic Potential of Extremophilic Microorganisms through the Integration of Metagenomics and Single-Cell Genomics

2020 ◽  
Author(s):  
Deepika Goyal ◽  
Shiv Swaroop ◽  
Janmejay Pandey
Keyword(s):  
Microbial Diversity ◽  
Single Cell ◽  
Molecular Mechanisms ◽  
Extreme Environments ◽  
Metabolic Potential ◽  
Single Cell Genomics ◽  
Metabolic Characteristics ◽  
Culture Independent ◽  
Synergistic Approach

Microorganisms thriving under extreme environments have proven to be an invaluable resource for metabolic products and processes. While studies carried out on microbial characterization of extremophilic environments during golden era of microbiology adapted a ‘reductionist approach’ and focused on isolation, purification and characterization of individual microbial isolates; the recent studies have implemented a holistic approach using both culture-dependent and culture-independent approaches for characterization of total microbial diversity of the extreme environments. Findings from these studies have unmistakably indicated that microbial diversity within extreme environments is much higher than anticipated. Consequently, unraveling the taxonomic and metabolic characteristics of microbial diversity in extreme environments has emerged as an imposing challenge in the field of microbiology and microbial biotechnology. To a great extent, this challenge has been addressed with inception and advancement of next-generation sequencing and computing methods for NGS data analyses. However, further it has been realized that in order to maximize the exploitation of genetic and metabolic diversity of extremophilic microbial diversity, the metagenomic approaches must be combined synergistically with single-cell genomics. A synergistic approach is expected to provide comprehensions into the biology of extremophilic microorganism, including their metabolic potential, molecular mechanisms of adaptations, unique genomic features including codon reassignments etc.

scholarly journals Gezielte Zellsortierung in der Einzelzellgenomik

BIOspektrum ◽  
2021 ◽  
Vol 27 (3) ◽  
pp. 274-276
Author(s):  
Morgan S. Sobol ◽  
Anne-Kristin Kaster
Keyword(s):  
Dark Matter ◽  
Single Cell ◽  
Cell Sorting ◽  
Genetic Information ◽  
Fluorescent Labeling ◽  
Single Cell Genomics ◽  
Rare Biosphere ◽  
Prokaryotic Genomes ◽  
Selection Of

AbstractSingle cell genomics (SCG) can provide reliable context for assembled genome fragments on the level of individual prokaryotic genomes and has rapidly emerged as an essential complement to cultivation-based and metagenomics research approaches. Targeted cell sorting approaches, which enable the selection of specific taxa by fluorescent labeling, compatible with subsequent single cell genomics offers an opportunity to access genetic information from rare biosphere members which would have otherwise stayed hidden as microbial dark matter.

scholarly journals What's New Is Old: Resolving the Identity of Leptothrix ochracea Using Single Cell Genomics, Pyrosequencing and FISH

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17769 ◽  
Author(s):  
Emily J. Fleming ◽  
Amy E. Langdon ◽  
Manuel Martinez-Garcia ◽  
Ramunas Stepanauskas ◽  
Nicole J. Poulton ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Genomics

scholarly journals The metabolic potential of the single cell genomes obtained from the Challenger Deep, Mariana Trench within the candidate superphylum Parcubacteria (OD1)

2017 ◽  
Vol 19 (7) ◽  
pp. 2769-2784 ◽  
Author(s):  
Rosa León-Zayas ◽  
Logan Peoples ◽  
Jennifer F. Biddle ◽  
Sheila Podell ◽  
Mark Novotny ◽  
...  
Keyword(s):  
Single Cell ◽  
Metabolic Potential ◽  
Mariana Trench ◽  
Challenger Deep

Development of a platform for single cell genomics using convex lens-induced confinement

Lab on a Chip ◽  
2015 ◽  
Vol 15 (14) ◽  
pp. 3013-3020 ◽  
Author(s):  
Sara Mahshid ◽  
Mohammed Jalal Ahamed ◽  
Daniel Berard ◽  
Susan Amin ◽  
Robert Sladek ◽  
...  
Keyword(s):  
Single Cell ◽  
Base Pair ◽  
Single Cell Analysis ◽  
Lab On A Chip ◽  
Cell Analysis ◽  
Next Generation ◽  
Single Cell Genomics ◽  
Convex Lens ◽  
Genomic Dnas

We present a lab-on-a-chip for the next generation of single-cell genomics, performing full-cycle single-cell analysis by demonstrating mega-base pair genomic DNAs in nanochannels extracted in situ.

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