scholarly journals Metabolic Engineering of Escherichia coli for Enhanced Production of Naringenin 7-Sulfate and Its Biological Activities

2018 ◽  
Vol 9 ◽  
Author(s):  
Luan L. Chu ◽  
Dipesh Dhakal ◽  
Hee J. Shin ◽  
Hye J. Jung ◽  
Tokutaro Yamaguchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Biological Activities ◽  
Enhanced Production

Metabolic engineering of Escherichia coli for the production of isoflavonoid‐4′‐ O ‐methoxides and their biological activities

2017 ◽  
Vol 66 (4) ◽  
pp. 484-493 ◽  
Author(s):  
Niranjan Koirala ◽  
Ramesh Prasad Pandey ◽  
Nguyen Huy Thuan ◽  
Gopal Prasad Ghimire ◽  
Hye Jin Jung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Biological Activities

scholarly journals Metabolic engineering of Saccharomyces cerevisiae for enhanced production of caffeic acid

2020 ◽  
Author(s):  
Pingping Zhou ◽  
Chunlei Yue ◽  
Bin Shen ◽  
Yi Du ◽  
Nannan Xu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Caffeic Acid ◽  
Acid Production ◽  
De Novo ◽  
Feedback Inhibition ◽  
Biological Activities ◽  
Acid Product ◽  
Enhanced Production ◽  
Microbial Biosynthesis

Abstract Background As a natural phenolic acid product of plant source, caffeic acid displays diverse biological activities and acts as an important precursor for the synthesis of other valuable compounds. Limitations in chemical synthesis or plant extraction of caffeic acid trigger interest in its microbial biosynthesis. Recently, Saccharomyces cerevisiae has been reported sporadically for biosynthesis of caffeic acid via free plasmid‑mediated pathway assembly. However, the production was far from satisfactory and even relied on the addition of precursor. Results In this study, we first established a controllable caffeic acid pathway by employing a modified GAL regulatory system in S. cerevisiae and realized de novo biosynthesis of 313.8 mg/L caffeic acid from glucose. Combinatorial engineering strategies including eliminating the tyrosine-induced feedback inhibition, deleting genes involved in competing pathways and overexpressing rate-limiting enzymes led to about 2.5-fold improvement in the caffeic acid production, reaching up to 769.3 mg/L in shake-flask cultures. To our knowledge, this is the highest ever reported titer of caffeic acid de novo synthesized by engineered yeast. Conclusions Caffeic acid production in S. cerevisiae strain was successfully improved by adopting a glucose-regulated GAL system and comprehensive metabolic engineering strategies. This work showed the prospect for microbial biosynthesis of caffeic acid and laid the foundation for constructing biosynthetic pathways of its derived metabolites.

Metabolic engineering for the production of chitooligosaccharides: advances and perspectives

2018 ◽  
Vol 2 (3) ◽  
pp. 377-388 ◽  
Author(s):  
Meixi Ling ◽  
Jianghua Li ◽  
Guocheng Du ◽  
Long Liu
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Biological Activities ◽  
Future Research ◽  
Microbial Fermentation ◽  
Chitosan Oligosaccharides ◽  
Pharmaceutical Industries ◽  
Environmentally Friendly Process ◽  
Hydrolysis Of ◽  
Related Compounds

Chitin oligosaccharides (CTOs) and its related compounds chitosan oligosaccharides (CSOs), collectively known as chitooligosaccharides (COs), exhibit numerous biological activities in applications in the nutraceutical, cosmetics, agriculture, and pharmaceutical industries. COs are currently produced by acid hydrolysis of chitin or chitosan, or enzymatic techniques with uncontrollable polymerization. Microbial fermentation by recombinant Escherichia coli, as an alternative method for the production of COs, shows new potential because it can produce a well-defined COs mixture and is an environmentally friendly process. In addition, Bacillus subtilis, a nonpathogenic, endotoxin-free, GRAS status bacterium, presents a new opportunity as a platform to produce COs. Here, we review the applications of COs and differences between CTOs and CSOs, summarize the current preparation approaches of COs, and discuss the future research potentials and challenges in the production of well-defined COs in B. subtilis by metabolic engineering.

Enhanced production of polysialic acid by metabolic engineering of Escherichia coli

2015 ◽  
Vol 99 (6) ◽  
pp. 2603-2611 ◽  
Author(s):  
Fang Chen ◽  
Yong Tao ◽  
Cheng Jin ◽  
Yang Xu ◽  
Bai-Xue Lin
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Polysialic Acid ◽  
Enhanced Production

Metabolic engineering of Escherichia coli for the production of malic acid

2008 ◽  
Vol 40 (2) ◽  
pp. 312-320 ◽  
Author(s):  
Soo Yun Moon ◽  
Soon Ho Hong ◽  
Tae Yong Kim ◽  
Sang Yup Lee
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Malic Acid
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