scholarly journals Overexpression of THI4 and HAP4 Improves Glucose Metabolism and Ethanol Production in Saccharomyces cerevisiae

2018 ◽  
Vol 9 ◽  
Author(s):  
Xinchi Shi ◽  
Yanan Zou ◽  
Yong Chen ◽  
Hanjie Ying
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Metabolism ◽  
Ethanol Production

scholarly journals Multicopy FZF1 (SUL1) Suppresses the Sulfite Sensitivity but Not the Glucose Derepression or Aberrant Cell Morphology of a grr1 Mutant of Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 511-521 ◽  
Author(s):  
Dorina Avram ◽  
Alan T Bakalinsky
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Glucose Metabolism ◽  
Cell Morphology ◽  
Glucose Repression ◽  
Carbon Sources ◽  
Single Copy ◽  
Aberrant Cell ◽  
Growth Defects ◽  
Putative Transcription Factor

Abstract An ssu2 mutation in Sacccharomyces cermisiae, previously shown to cause sulfite sensitivity, was found to be allelic to GRR1, a gene previously implicated in glucose repression. The suppressor rgt1, which suppresses the growth defects of grr1 strains on glucose, did not fully suppress the sensitivity on glucose or nonglucose carbon sources, indicating that it is not strictly linked to a defect in glucose metabolism. Because the Cln1 protein was previously shown to be elevated in grr1 mutants, the effect of CLN1 overexpression on sulfite sensitivity was investigated. Overexpression in GRR1 cells resulted in sulfite sensitivity, suggesting a connection between CLN1 and sulfite metabolism. Multicopy FZF1, a putative transcription factor, was found to suppress the sulfite sensitive phenotype of grr1 strains, but not the glucose derepression or aberrant cell morphology. Multicopy FZF1 was also found to suppress the sensitivity of a number of other unrelated sulfite-sensitive mutants, but not that of ssu1 or met20, implying that FZF1 may act through Ssulp and Met20p. Disruption of FZF1 resulted in sulfite sensitivity when the construct was introduced in single copy at the FZF1 locus in a GRR1 strain, providing evidence that FZF1 is involved in sulfite metabolism.

scholarly journals Effects of Ultrasound on Fermentation of Glucose to Ethanol by Saccharomyces cerevisiae

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 16 ◽  
Author(s):  
Luis Huezo ◽  
Ajay Shah ◽  
Frederick Michel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mass Transfer ◽  
Glucose Uptake ◽  
Ethanol Production ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Inhibitory Effects ◽  
Negative Effects ◽  
Intraparticle Mass Transfer ◽  
Improve Mass Transfer

Previous studies have shown that pretreatment of corn slurries using ultrasound improves starch release and ethanol yield during biofuel production. However, studies on its effects on the mass transfer of substrates and products during fermentation have shown that it can have both beneficial and inhibitory effects. In this study, the effects of ultrasound on mass transfer limitations during fermentation were examined. Calculation of the external and intraparticle observable moduli under a range of conditions indicate that no external or intraparticle mass transfer limitations should exist for the mass transfer of glucose, ethanol, or carbon dioxide. Fermentations of glucose to ethanol using Saccharomyces cerevisiae were conducted at different ultrasound intensities to examine its effects on glucose uptake, ethanol production, and yeast population and viability. Four treatments were compared: direct ultrasound at intensities of 23 and 32 W/L, indirect ultrasound (1.4 W/L), and no-ultrasound. Direct and indirect ultrasound had negative effects on yeast performance and viability, and reduced the rates of glucose uptake and ethanol production. These results indicate that ultrasound during fermentation, at the levels applied, is inhibitory and not expected to improve mass transfer limitations.

Some physiological parameters for ethanol production from beet molasses by Saccharomyces cerevisiae Y-7

1992 ◽  
Vol 42 (3) ◽  
pp. 183-189 ◽  
Author(s):  
A.H. El-Refai ◽  
M.S. El-Abyad ◽  
A.I. El-Diwany ◽  
L.A. Sallam ◽  
Reda F. Allam
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Physiological Parameters ◽  
Beet Molasses

Genetic Enhancement of Saccharomyces Cerevisiae for First and Second Generation Ethanol Production

2017 ◽  
pp. 239-279
Author(s):  
Fernanda Bravim ◽  
Melina Campagnaro Farias ◽  
Oeber De Freitas Quadros ◽  
Patricia Machado Bueno Fernandes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Second Generation ◽  
Genetic Enhancement ◽  
First And Second Generation ◽  
Second Generation Ethanol

scholarly journals Supplementation of sugarcane molasses for maximization of ethanol production by Saccharomyces cerevisiae using response surface method

ScienceAsia ◽  
2019 ◽  
Vol 45 (3) ◽  
pp. 229 ◽  
Author(s):  
Pongpannee Phomikhet ◽  
Wanlapa Lorliam ◽  
Suthep Thaniyavarn ◽  
Somboon Tanasupawat ◽  
Ancharida Savarajara
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Response Surface ◽  
Response Surface Method ◽  
Sugarcane Molasses ◽  
Surface Method
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