scholarly journals Plant Growth Promotion Under Water: Decrease of Waterlogging-Induced ACC and Ethylene Levels by ACC Deaminase-Producing Bacteria

2018 ◽  
Vol 9 ◽  
Author(s):  
Sajid Ali ◽  
Won-Chan Kim
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase

scholarly journals Quorum Sensing System Affects the Plant Growth Promotion Traits of Serratia fonticola GS2

2020 ◽  
Vol 11 ◽  
Author(s):  
Byung Kwon Jung ◽  
Jerald Conrad Ibal ◽  
Huy Quang Pham ◽  
Min-Chul Kim ◽  
Gun-Seok Park ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Biofilm Formation ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Deletion Mutants ◽  
Phenotypic Data ◽  
Collective Behaviors ◽  
Pgp Activities

Quorum sensing (QS) enables bacteria to organize gene expression programs, thereby coordinating collective behaviors. It involves the production, release, and population-wide detection of extracellular signaling molecules. The cellular processes regulated by QS in bacteria are diverse and may be used in mutualistic coordination or in response to changing environmental conditions. Here, we focused on the influence of the QS-dependent genes of our model bacterial strain Serratia fonticola GS2 on potential plant growth promoting (PGP) activities including indole-3-acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and biofilm formation. Based on genomic and phenotypic experimental data we identified and investigated the function of QS genes in the genome of the model strain. Our gene deletion study confirmed the biological functionality of the QS auto-inducer (gloI) and receptor (gloR) on potential PGP activities of GS2. A transcriptomic approach was also undertaken to understand the role of QS genes in regulation of genes primarily involved in PGP activities (IAA, ACC deaminase activity, and biofilm formation). Both transcriptomic and phenotypic data revealed that the QS-deletion mutants had considerably less PGP activities, as compared to the wild type. In addition, in vivo plant experiments showed that plants treated with GS2 had significantly higher growth rates than plants treated with the QS-deletion mutants. Overall, our results showed how QS-dependent genes regulate the potential PGP activities of GS2. This information may be helpful in understanding the relationship between QS-dependent genes and the PGP activity of bacteria, which aid in the production of practical bio-fertilizers for plant growth promotion.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

Isolation, selection, and characterization of beneficial rhizobacteria from pea, lentil, and chickpea grown in western Canada

2008 ◽  
Vol 54 (4) ◽  
pp. 248-258 ◽  
Author(s):  
Russell K. Hynes ◽  
Grant C.Y. Leung ◽  
Danielle L.M. Hirkala ◽  
Louise M. Nelson
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Growth Promotion ◽  
Profile Analysis ◽  
Acc Deaminase ◽  
Plant Growth Promoting ◽  
Growth Promoting

The use of beneficial soil microorganisms as agricultural inputs for improved crop production requires selection of rhizosphere-competent microorganisms with plant growth-promoting attributes. A collection of 563 bacteria originating from the roots of pea, lentil, and chickpea grown in Saskatchewan was screened for several plant growth-promoting traits, for suppression of legume fungal pathogens, and for plant growth promotion. Siderophore production was detected in 427 isolates (76%), amino-cyclopropane-1-carboxylic acid (ACC) deaminase activity in 29 isolates (5%), and indole production in 38 isolates (7%). Twenty-six isolates (5%) suppressed the growth of Pythium sp. strain p88-p3, 40 isolates (7%) suppressed the growth of Fusarium avenaceum , and 53 isolates (9%) suppressed the growth of Rhizoctonia solani CKP7. Seventeen isolates (3%) promoted canola root elongation in a growth pouch assay, and of these, 4 isolates promoted the growth of lentil and one isolate promoted the growth of pea. Fatty acid profile analysis and 16S rRNA sequencing of smaller subsets of the isolates that were positive for the plant growth-promotion traits tested showed that 39%–42% were members of the Pseudomonadaceae and 36%–42% of the Enterobacteriaceae families. Several of these isolates may have potential for development as biofertilizers or biopesticides for western Canadian legume crops.

scholarly journals Genomic Analysis of the 1-Aminocyclopropane-1-Carboxylate Deaminase-Producing Pseudomonas thivervalensis SC5 Reveals Its Multifaceted Roles in Soil and in Beneficial Interactions With Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Francisco X. Nascimento ◽  
Paola Urón ◽  
Bernard R. Glick ◽  
Admir Giachini ◽  
Márcio J. Rossi
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Genomic Analysis ◽  
Acc Deaminase ◽  
Quorum Quenching ◽  
Cucumber Plant

Beneficial 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing bacteria promote plant growth and stress resistance, constituting a sustainable alternative to the excessive use of chemicals in agriculture. In this work, the increased plant growth promotion activity of the ACC deaminase-producing Pseudomonas thivervalensis SC5, its ability to limit the growth of phytopathogens, and the genomics behind these important properties are described in detail. P. thivervalensis SC5 displayed several active plant growth promotion traits and significantly increased cucumber plant growth and resistance against salt stress (100mmol/L NaCl) under greenhouse conditions. Strain SC5 also limited the in vitro growth of the pathogens Botrytis cinerea and Pseudomonas syringae DC3000 indicating active biological control activities. Comprehensive analysis revealed that P. thivervalensis SC5 genome is rich in genetic elements involved in nutrient acquisition (N, P, S, and Fe); osmotic stress tolerance (e.g., glycine-betaine, trehalose, and ectoine biosynthesis); motility, chemotaxis and attachment to plant tissues; root exudate metabolism including the modulation of plant phenolics (e.g., hydroxycinnamic acids), lignin, and flavonoids (e.g., quercetin); resistance against plant defenses (e.g., reactive oxygens species-ROS); plant hormone modulation (e.g., ethylene, auxins, cytokinins, and salicylic acid), and bacterial and fungal phytopathogen antagonistic traits (e.g., 2,4-diacetylphloroglucinol, HCN, a fragin-like non ribosomal peptide, bacteriocins, a lantipeptide, and quorum-quenching activities), bringing detailed insights into the action of this versatile plant-growth-promoting bacterium. Ultimately, the combination of both increased plant growth promotion/protection and biological control abilities makes P. thivervalensis SC5 a prime candidate for its development as a biofertilizer/biostimulant/biocontrol product. The genomic analysis of this bacterium brings new insights into the functioning of Pseudomonas and their role in beneficial plant-microbe interactions.

scholarly journals Metagenomic insights into plant growth promoting genes inherent in bacterial endophytes of Emilia sonchifolia (Linn.) DC

2021 ◽  
Vol 8 (sp1) ◽  
pp. 6-16
Author(s):  
Sithara K Urumbil ◽  
M Anilkumar
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Host Plants ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Metabolic Potential ◽  
Illumina Hiseq ◽  
Characteristic Functional ◽  
Plant Growth Hormones ◽  
Metagenome Analysis

Studies on the genome of endophytes reveal the metabolic potential of endophytic microbiome including both culturable and unculturable fractions. The metagenome analysis through the Illumina HiSeq platform gives access to the genetic data encrypted for the molecular machinery, which takes part in plant growth promotion activity of the endophyte in various aspects including production of plant growth hormones and enhancing nutrient availability for the host plant. The present work was undertaken to identify the genes involved in plant growth promotion activities from the endophytes of Emilia sonchifolia(Linn.) DC. through metagenome analysis. Metagenomic studies include the analysis of functional annotations which aid in the detection of biocatalysts taking part in the metabolic pathway of host plants. The annotations of expressed genes in different databases like NCBI Nr, KEGG, eggnog and CAZy resulted in enlisting the vast array of information on the genetic diversity of the endophytic microbiome. The metagenome analysis of endophytic bacteria from the medicinal plant E.sonchifolia unveiled characteristic functional genes involved in plant growth promotion such as nitrogen metabolism (nif) and siderophore production (enterobactin category), ipdC and tnaA (IAA producing), ACC deaminase coding genes (regulation of elevated ethylene levels in host tissues), Mo-Nitrogenase, nitrous-oxide reductase (nosZ), nitrate reductase (narG, napA), nitrite reductase (nirD) (nutrient assimilation and absorption) enterobactin siderophore synthetase components F and D and acid phosphatase genes. This clearly explains the effective plant-microbe relationship and the role of bacterial endophytic microbes in regulating the growth of host plants.

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