scholarly journals Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis

2018 ◽  
Vol 9 ◽  
Author(s):  
Zhenchao Zhang ◽  
Yuhua Li ◽  
Mingyong Wang ◽  
Qing Xie ◽  
Pengju Li ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Vaccine ◽  
Immune Protection ◽  
Rhoptry Protein

Molecular characterization and protective immunity of rhoptry protein 35 (ROP35) of Toxoplasma gondii as a DNA vaccine

2018 ◽  
Vol 260 ◽  
pp. 12-21 ◽  
Author(s):  
Zhenchao Zhang ◽  
Yuhua Li ◽  
Yuanyuan Liang ◽  
Shuai Wang ◽  
Qing Xie ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization ◽  
Dna Vaccine ◽  
Protective Immunity ◽  
Rhoptry Protein

scholarly journals Protective Efficacy of a Toxoplasma gondii Rhoptry Protein 13 Plasmid DNA Vaccine in Mice

2012 ◽  
Vol 19 (12) ◽  
pp. 1916-1920 ◽  
Author(s):  
Pei-Yuan Wang ◽  
Zi-Guo Yuan ◽  
Eskild Petersen ◽  
Jie Li ◽  
Xiu-Xiang Zhang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Vaccine ◽  
Cellular Response ◽  
Protective Efficacy ◽  
Eukaryotic Expression ◽  
Economic Losses ◽  
Type I ◽  
Content Type ◽  
Strain Type ◽  
Rhoptry Protein

ABSTRACTToxoplasma gondiiis an obligate intracellular parasite infecting humans and other warm-blooded animals, resulting in serious public health problems and economic losses worldwide. Rhoptries are involved inT. gondiiinvasion and host cell interaction and have been implicated as important virulence factors. In the present study, a DNA vaccine expressing rhoptry protein 13 (ROP13) ofT. gondiiinserted into eukaryotic expression vector pVAX I was constructed, and the immune protection it induced in Kunming mice was evaluated. Kunming mice were immunized intramuscularly with pVAX-ROP13 and/or with interleukin-18 (IL-18). Then, we evaluated the immune response using a lymphoproliferative assay, cytokine and antibody measurements, and the survival times of mice challenged with the virulentT. gondiiRH strain (type I) and the cyst-forming PRU strain (type II). The results showed that pVAX-ROP13 alone or with pVAX/IL-18 induced a high level of specific anti-T. gondiiantibodies and specific lymphocyte proliferative responses. Coinjection of pVAX/IL-18 significantly increased the production of gamma interferon (IFN-γ), IL-2, IL-4, and IL-10. Further, challenge experiments showed that coimmunization of pVAX-ROP13 with pVAX/IL-18 significantly (P< 0.05) increased survival time (32.3 ± 2.7 days) compared with pVAX-ROP13 alone (24.9 ± 2.3 days). Immunized mice challenged withT. gondiicysts (strain PRU) had a significant reduction in the number of brain cysts, suggesting that ROP13 could trigger a strong humoral and cellular response againstT. gondiicyst infection and that it is a potential vaccine candidate against toxoplasmosis, which provided the foundation for further development of effective vaccines againstT. gondii.

scholarly journals In Silico Prediction of T and B Cell Epitopes of SAG1-Related Sequence 3 (SRS3) Gene for Developing Toxoplasma gondii Vaccine

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Abolfazl Mirzadeh ◽  
Geita Saadatnia ◽  
Majid Golkar ◽  
Jalal Babaie ◽  
Samira Amiri ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Vaccine ◽  
In Silico ◽  
Effective Vaccine ◽  
T Cell Epitopes ◽  
In Silico Prediction ◽  
Vaccine Strategy ◽  
Related Sequence ◽  
Ideal Tool ◽  
Major Surface

: Toxoplasmosis is a worldwide infection that can lead to serious problems in immune-compromised individuals and fetuses. A DNA vaccine strategy would be an ideal tool against Toxoplasma gondii. One of the necessary measures to provide an effective vaccine is the selection of proteins with high antigenicity. The SAG1-related sequence 3 (SRS3) protein is a major surface antigen in T. gondii that can be used as a vaccine candidate. In the present study, bioinformatics and computational methods were utilized to predict protein characteristics, as well as secondary and tertiary structures. The in silico approach is highly suited to analyze, design, and evaluate DNA vaccine strategies. Hence, in silico prediction was used to identify B and T cell epitopes and compare the antigenicity of SRS3 and other candidate genes of Toxoplasma previously applied in the production of vaccines. The results of the analysis theoretically showed that SRS3 has multiple epitopes with high antigenicity, proposing that SRS3 is a promising immunogenic candidate for the development of DNA vaccines against toxoplasmosis.

Virus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection

Vaccine ◽  
2018 ◽  
Vol 36 (38) ◽  
pp. 5692-5700 ◽  
Author(s):  
Su-Hwa Lee ◽  
Hae-Ji Kang ◽  
Dong-Hun Lee ◽  
Sang-Moo Kang ◽  
Fu-Shi Quan
Keyword(s):  
Toxoplasma Gondii ◽  
Virus Like Particle ◽  
Microneme Protein ◽  
Rhoptry Protein

The virulence-related rhoptry protein 5 (ROP5) of Toxoplasma Gondii is a novel vaccine candidate against toxoplasmosis in mice

Vaccine ◽  
2013 ◽  
Vol 31 (41) ◽  
pp. 4578-4584 ◽  
Author(s):  
Bin Zheng ◽  
Shaohong Lu ◽  
Qunbo Tong ◽  
Qingming Kong ◽  
Di Lou
Keyword(s):  
Toxoplasma Gondii ◽  
Vaccine Candidate ◽  
Rhoptry Protein

scholarly journals A novel dual promoter DNA vaccine induces CD8+ response against Toxoplasma gondii sporozoite specific surface protein “SporoSAG” through non-apoptotic cells

2014 ◽  
Vol 3 ◽  
pp. 81-88 ◽  
Author(s):  
Sultan Gülçe İz ◽  
Mert Döşkaya ◽  
Ayşe Caner ◽  
Aysu Değirmenci Döşkaya ◽  
Fernando Rodriguez ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Specific Surface ◽  
Dna Vaccine ◽  
Surface Protein ◽  
Apoptotic Cells ◽  
Dual Promoter ◽  
Promoter Dna
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