scholarly journals Convergent Loss of ABC Transporter Genes From Clostridioides difficile Genomes Is Associated With Impaired Tyrosine Uptake and p-Cresol Production

2018 ◽  
Vol 9 ◽  
Author(s):  
Matthias Steglich ◽  
Julia D. Hofmann ◽  
Julia Helmecke ◽  
Johannes Sikorski ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Clostridioides Difficile ◽  
Tyrosine Uptake ◽  
Abc Transporter Genes

5030 POSTER Expression Profile of ABC Transporter Genes in Breast Carcinoma

2011 ◽  
Vol 47 ◽  
pp. S339-S340
Author(s):  
V. Hlavac ◽  
R. Vaclavikova ◽  
M. Ehrlichova ◽  
I. Hlavata ◽  
V. Pecha ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Abc Transporter ◽  
Expression Profile ◽  
Abc Transporter Genes

scholarly journals Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s.

Insects ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 66 ◽  
Author(s):  
Valentina Mastrantonio ◽  
Marco Ferrari ◽  
Agata Negri ◽  
Tommaso Sturmo ◽  
Guido Favia ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Abc Transporter ◽  
Abc Transporters ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Larval Mortality ◽  
Expression Patterns ◽  
Main Tool ◽  
Gene Expression Response ◽  
Abc Transporter Genes

Insecticides remain a main tool for the control of arthropod vectors. The urgency to prevent the insurgence of insecticide resistance and the perspective to find new target sites, for the development of novel molecules, are fuelling the study of the molecular mechanisms involved in insect defence against xenobiotic compounds. In this study, we have investigated if ATP-binding cassette (ABC) transporters, a major component of the defensome machinery, are involved in defence against the insecticide permethrin, in susceptible larvae of the malaria vector Anopheles gambiae sensu stricto. Bioassays were performed with permethrin alone, or in combination with an ABC transporter inhibitor. Then we have investigated the expression profiles of five ABC transporter genes at different time points following permethrin exposure, to assess their expression patterns across time. The inhibition of ABC transporters increased the larval mortality by about 15-fold. Likewise, three genes were up-regulated after exposure to permethrin, showing different patterns of expression across the 48 h. Our results provide the first evidences of ABC transporters involvement in defence against a toxic in larvae of An. gambiae s.s. and show that the gene expression response is modulated across time, being continuous, but stronger at the earliest and latest times after exposure.

scholarly journals A Low-Affinity Penicillin-Binding Protein 2x Variant Is Required for Heteroresistance in Streptococcus pneumoniae

2014 ◽  
Vol 58 (7) ◽  
pp. 3934-3941 ◽  
Author(s):  
Hansjürg Engel ◽  
Moana Mika ◽  
Dalia Denapaite ◽  
Regine Hakenbeck ◽  
Kathrin Mühlemann ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Abc Transporter ◽  
Binding Protein ◽  
Population Analysis ◽  
Resistance Testing ◽  
Penicillin Binding Protein ◽  
Secondary Resistance ◽  
Content Type ◽  
Abc Transporter Genes

ABSTRACTHeteroresistance to penicillin inStreptococcus pneumoniaeis the ability of subpopulations to grow at a higher antibiotic concentration than expected from the MIC. This may render conventional resistance testing unreliable and lead to therapeutic failure. We investigated the role of the primary β-lactam resistance determinants, penicillin-binding protein 2b (PBP2b) and PBP2x, and the secondary resistance determinant PBP1a in heteroresistance to penicillin. Transformants containing PBP genes from the heteroresistant strain Spain23F2349in the nonheteroresistant strain R6 background were tested for heteroresistance by population analysis profiling (PAP). We found thatpbp2x, but notpbp2borpbp1aalone, conferred heteroresistance to R6. However, a change ofpbp2xexpression was not observed, and therefore, expression does not correlate with an increased proportion of resistant subpopulations. In addition, the influence of the CiaRH system, mediating PBP-independent β-lactam resistance, was assessed by PAP onciaRdisruption mutants but revealed no heteroresistant phenotype. We also showed that the highly resistant subpopulations (HOM*) of transformants containing low-affinitypbp2xundergo an increase in resistance upon selection on penicillin plates that partially reverts after passaging on selection-free medium. Shotgun proteomic analysis showed an upregulation of phosphate ABC transporter subunit proteins encoded bypstS,phoU,pstB, andpstCin these highly resistant subpopulations. In conclusion, the presence of low-affinitypbp2xenables certain pneumococcal colonies to survive in the presence of β-lactams. Upregulation of phosphate ABC transporter genes may represent a reversible adaptation to antibiotic stress.

Regulation of ABC transporter genes

2009 ◽  
Vol 189 ◽  
pp. S18
Author(s):  
Ronald Oude Elferink
Keyword(s):  
Abc Transporter ◽  
Abc Transporter Genes

scholarly journals New Nomenclature of Human ABC Transporter Genes.

2000 ◽  
Vol 15 (1) ◽  
pp. 8-19
Author(s):  
Toshihisa ISHIKAWA ◽  
Rando ALLIKIMETS ◽  
Michael DEAN ◽  
Christopher HIGGINS ◽  
Victor LING ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Abc Transporter Genes

scholarly journals Ligands of Thermophilic ABC Transporters Encoded in a Newly Sequenced Genomic Region of Thermotoga maritima MSB8 Screened by Differential Scanning Fluorimetry

2011 ◽  
Vol 77 (18) ◽  
pp. 6395-6399 ◽  
Author(s):  
Nathalie Boucher ◽  
Kenneth M. Noll
Keyword(s):  
Abc Transporter ◽  
Thermotoga Maritima ◽  
Binding Constants ◽  
Genomic Region ◽  
Genomic Research ◽  
Gene Encoding ◽  
Content Type ◽  
Differential Scanning Fluorimetry ◽  
Beta Glucosidase ◽  
Abc Transporter Genes

ABSTRACTThe chromosome ofThermotoga maritimastrain MSB8 was found to have an 8,870-bp region that is not present in its published sequence. The isolate that was sequenced by The Institute for Genomic Research (TIGR) in 1999 is apparently a laboratory variant of the isolate deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSM 3109) in 1986. This newly sequenced region from the DSMZ culture was located between TM1848 (cbp, cellobiose phosphorylase) and TM1847 (the 3′ end of a truncated ROK regulator). The new region contained seven genes: a beta glucosidase gene (bglA), three trehalose ABC transporter genes (treEFG), three xylose ABC transporter genes (xylE2F2K2), and the 5′ end of a gene encoding the ROK regulator TM1847. We present a new differential scanning fluorimetry method using a low pH that was necessary to screen potential ligands of these exceptionally thermostable periplasmic substrate-binding proteins. This method showed that trehalose, sucrose, and glucose stabilized TreE, and their binding was confirmed by measuring changes in intrinsic fluorescence upon ligand binding. Binding constants of 0.024 μM, 0.300 μM, and 56.78 μM at 60°C, respectively, were measured. XylE2 ligands were similarly determined and xylose, glucose, and fucose bound withKd(dissociation constant) values of 0.042 μM, 0.059 μM, and 1.436 μM, respectively. Since there is no discernible phenotypic difference between the TIGR isolate and the DSMZ isolate despite the variance in their genomes, we propose that they be called genomovars:T. maritimaMSB8 genomovar TIGR andT. maritimaMSB8 genomovar DSM 3109, respectively.

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