Killing Effects of an Isolated Serratia marcescens KH-001 on Diaphorina citri via Lowering the Endosymbiont Numbers

Evaluating the Biological Potential of Prodigiosin from Serratia Marcescens KH-001 Against Asian Citrus Psyllid

Journal of Economic Entomology ◽

10.1093/jee/toab041 ◽

2021 ◽

Author(s):

Wei Hu ◽

Rongkun Zheng ◽

Yihong Liao ◽

Fan Kuang ◽

Zhaolan Yang ◽

...

Keyword(s):

Serratia Marcescens ◽

Diaphorina Citri ◽

Antifeedant Activity ◽

Control Efficacy ◽

Egg Hatch ◽

Biological Potential ◽

Citrus Orchards ◽

Citrus Leaves ◽

Increasing Temperature ◽

Effective Compound

Abstract The insecticides of microbial origin may be promising biocontrol agents to control pests. In this study, a major metabolite was extracted and purified from Serratia marcescens KH-001 and identified as prodigiosin with a molecule weight of 324.2094, and our primary aim was to evaluate the effects of prodigiosin against Diaphorina citri Kuwayama (Hemiptera: Liviidae) in the laboratory and in the field. Toxicity of purified prodigiosin to nymphs increased with increasing temperature from 25 to 35°C. The laboratory experiment results observed inhibition effects of purified prodigiosin at LC50 on oviposition and egg hatch of D. citri at 30°C. Furthermore, adults that fed on citrus leaves treated with LC20 and LC50 solution of purified prodigiosin at 30°C excreted less honeydew (28–35%) compared with controls, suggesting moderate antifeedant activity of prodigiosin. The results of field experiment showed that 10% prodigiosin emulsifiable concentrate exhibited better control efficacy (70–100%) at three concentrations (100, 200, and 500 mg/liter) in July and August than in October. These data indicate that prodigiosin will be a highly effective compound for the control of D. citri depending on the prevailing temperature conditions and may become a commercially available product to be widely used in citrus orchards.

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Serratia marcescens endocarditis. Report of a patient

Archives of Internal Medicine ◽

10.1001/archinte.125.6.1038 ◽

1970 ◽

Vol 125 (6) ◽

pp. 1038-1040 ◽

Cited By ~ 1

Author(s):

J. C. Williams

Keyword(s):

Serratia Marcescens

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Serratia marcescens septicemia

Archives of Internal Medicine ◽

10.1001/archinte.121.2.145 ◽

1968 ◽

Vol 121 (2) ◽

pp. 145-150 ◽

Cited By ~ 13

Author(s):

W. H. Dodson

Keyword(s):

Serratia Marcescens

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Thysanoptera diversity associated with Mexican lemon (Citrus aurantifolia Christm.)

RECUBRIMIENTO COMESTIBLE CON BASE DE PIÑON MEXICANO (Jatropha curcas) SOBRE LA CALIDAD DE CHAYOTE MINIMAMENTE PROCESADO ◽

10.32854/agrop.vi.1761 ◽

2020 ◽

Vol 13 (9) ◽

Author(s):

Francisco Javier Avendaño-Gutiérrez ◽

Salvador Aguirre Paleo ◽

Alejandro Morales Hernández ◽

Venecia Quesadas-Béjar

Keyword(s):

Biosphere Reserve ◽

Simple Random Sampling ◽

Diversity Indices ◽

Diaphorina Citri ◽

Program Calculation ◽

Homogeneous Relation ◽

Candidatus Liberibacter ◽

Scirtothrips Citri ◽

Species Richness And Abundance ◽

First Time

Objective: To calculate the monthly relative abundance of Thysanoptera species, according to the Margalef, Simpson and Shannon-Wiener diversity indices. Design / Methodology / Approach: The work was carried out in three geographic units with conventional management, during January-December, 2019 in the Reserva de la Biosfera Zicuirán-Infiernillo (Biosphere Reserve), Michoacán, Mexico. In each unit, 10 trees were selected through simple random sampling. Thrips counts were performed on ten shoots per tree every 15 d, for a total of 7200 shoots in the three geographic units. Thysanoptera individuals were placed in entomological jars. The variables were: number of thrips collected per shoot in sampled tree and geographic unit (orchard). To estimate the specific richness and structure of species, the program "calculation of diversity indices DIVERS" was used. Results: In the three geographical units studied, the recorded presence of Thysanoptera accounted for 12 to 17 species. For Nueva Italia 12 recorded species, two were permanent (16.66%), five abundant (41.66%), one scarce (8.3%) and four rare (33.33%). In Zicuirán, three species were permanent (17.64%), six abundant (35.29%), two scarce (11.76%) and six rare (35.29%). In Los Hoyos, four species were permanent (26.66%), four abundant (26.66%) and seven rare (46.66%). The abundance of species was represented by the genus Frankliniella and the species Scolothrips sexmaculatus and Scirtothrips citri. The highest species richness and abundance was found from January to May. In October and November, the value of the calculated indices was zero, which shows less richness and abundance of individuals. The best species uniformity was recorded during January and December, which meant a more stable and homogeneous relation. Study limitations/Implications. Pest resurgence, presence of Candidatus Liberibacter spp. and its vector Diaphorina citri. Findings / Conclusions: in Nueva Italia, 12 species were taxonomically determined; in Los Hoyos 15, and in Zicuirán 17 species, which are reported for the first time in the state of Michoacán, Mexico. At the geographic unit "Los Hoyos" diversity was higher, uniform and stable.

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