scholarly journals Phenalen-1-one-Mediated Antimicrobial Photodynamic Therapy: Antimicrobial Efficacy in a Periodontal Biofilm Model and Flow Cytometric Evaluation of Cytoplasmic Membrane Damage

2018 ◽  
Vol 9 ◽  
Author(s):  
Fabian Cieplik ◽  
Viktoria-Sophia Steinwachs ◽  
Denise Muehler ◽  
Karl-Anton Hiller ◽  
Thomas Thurnheer ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cytoplasmic Membrane ◽  
Membrane Damage ◽  
Antimicrobial Efficacy ◽  
Antimicrobial Photodynamic Therapy ◽  
Biofilm Model ◽  
Flow Cytometric ◽  
Flow Cytometric Evaluation

scholarly journals Phenalen-1-One-Mediated Antimicrobial Photodynamic Therapy and Chlorhexidine Applied to a Novel Caries Biofilm Model

2018 ◽  
Vol 52 (6) ◽  
pp. 447-453 ◽  
Author(s):  
Fabian Cieplik ◽  
Fabian Wimmer ◽  
Denise Muehler ◽  
Thomas Thurnheer ◽  
Georgios N. Belibasakis ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
High Throughput ◽  
High Throughput Screening ◽  
Antimicrobial Efficacy ◽  
Antimicrobial Photodynamic Therapy ◽  
Actinomyces Naeslundii ◽  
Biofilm Model ◽  
Pronounced Reduction

Antimicrobial photodynamic therapy (aPDT) may be useful as a supportive antimicrobial measure for caries-active subjects. In this study, the antimicrobial efficacy of aPDT with a phenalen-1-one photosensitizer was evaluated in a novel in vitro biofilm model comprising Actinomyces naeslundii, Actinomyces odontolyticus, and Streptococcus mutans and was compared to chlorhexidine. The proposed biofilm model allows high-throughput screening for antimicrobial efficacy while exhibiting a differentiated response to different antimicrobial approaches. While chlorhexidine 0.2% showed a reduction of ≈4 log10 for all species, aPDT led to a more pronounced reduction of S. mutans (2.8 log10) than of Actinomyces spp. (1.2 or 1.3 log10). A similar effect was also observed in monospecies biofilms. Therefore, aPDT may be more effective against S. mutans than against Actinomyces spp. when in biofilms, and this antimicrobial approach merits further investigations.

scholarly journals Antifungal Effect of Antimicrobial Photodynamic Therapy Mediated by Haematoporphyrin Monomethyl Ether and Aloe Emodin on Malassezia furfur

2021 ◽  
Vol 12 ◽  
Author(s):  
Zixin Cui ◽  
Miaomiao Zhang ◽  
Songmei Geng ◽  
Xinwu Niu ◽  
Xiaopeng Wang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Monomethyl Ether ◽  
Antimicrobial Efficacy ◽  
Irradiation Condition ◽  
Dependent Manner ◽  
Antimicrobial Photodynamic Therapy ◽  
Malassezia Furfur ◽  
Dermatological Diseases ◽  
Intracellular Ros ◽  
Aloe Emodin

Infectious dermatological diseases caused by Malassezia furfur are often chronic, recurrent, and recalcitrant. Current therapeutic options are usually tedious, repetitive, and associated with adverse effects. Alternatives that broaden the treatment options and reduce side effects for patients are needed. Antimicrobial photodynamic therapy (aPDT) is an emerging approach that is quite suitable for superficial infections. The aim of this study is to investigate the antimicrobial efficacy and effect of aPDT mediated by haematoporphyrin monomethyl ether (HMME) and aloe emodin (AE) on clinical isolates of M. furfur in vitro. The photodynamic antimicrobial efficacy of HMME and AE against M. furfur was assessed by colony forming unit (CFU) assay. The uptake of HMME and AE by M. furfur cells was investigated by fluorescence microscopy. Reactive oxygen species (ROS) probe and flow cytometry were employed to evaluate the intracellular ROS level. The effect of HMME and AE-mediated aPDT on secreted protease and lipase activity of M. furfur was also investigated. The results showed that HMME and AE in the presence of light effectively inactivated M. furfur cells in a photosensitizer (PS) concentration and light energy dose-dependent manner. AE exhibited higher antimicrobial efficacy against M. furfur than HMME under the same irradiation condition. HMME and AE-mediated aPDT disturbed the fungal cell envelop, significantly increased the intracellular ROS level, and effectively inhibited the activity of secreted protease and lipase of M. furfur cells. The results suggest that HMME and AE have potential to serve as PSs in the photodynamic treatment of dermatological diseases caused by M. furfur, but further ex vivo or in vivo experiments are needed to verify that they can meet the requirements for clinical practice.

The impact of antimicrobial photodynamic therapy in an artificial biofilm model

2011 ◽  
Vol 27 (3) ◽  
pp. 615-620 ◽  
Author(s):  
Martin Schneider ◽  
Gregor Kirfel ◽  
Michael Berthold ◽  
Matthias Frentzen ◽  
Felix Krause ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Biofilm Model ◽  
Artificial Biofilm ◽  
The Impact

The impact of laser irradiation during antimicrobial photodynamic therapy in an artificial biofilm model

2011 ◽  
Author(s):  
Martin Schneider ◽  
Gregor Kirfel ◽  
Michael Berthold ◽  
Olivier Brede ◽  
Matthias Frentzen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Laser Irradiation ◽  
Antimicrobial Photodynamic Therapy ◽  
Biofilm Model ◽  
Artificial Biofilm ◽  
The Impact

The impact of antimicrobial photodynamic therapy on Streptococcus mutans in an artificial biofilm model

2010 ◽  
Author(s):  
Martin Schneider ◽  
Gregor Kirfel ◽  
Felix Krause ◽  
Michael Berthold ◽  
Olivier Brede ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
Antimicrobial Photodynamic Therapy ◽  
Biofilm Model ◽  
Artificial Biofilm ◽  
The Impact

Regenerative treatment of peri-implantitis following implant surface decontamination with titanium brush and antimicrobial photodynamic therapy: a case series with reentry

2020 ◽  
Author(s):  
Pier Poli ◽  
Francisley Avila Souza ◽  
Mattia Manfredini ◽  
Carlo Maiorana ◽  
Mario Beretta
Keyword(s):  
Photodynamic Therapy ◽  
Clinical Case ◽  
Case Series ◽  
Antimicrobial Photodynamic Therapy ◽  
Implant Surface ◽  
Surface Decontamination

Not required for Clinical case letters according to the authors' guidelines.

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