scholarly journals Lipid Bodies as Sites of Prostaglandin E2 Synthesis During Chagas Disease: Impact in the Parasite Escape Mechanism

2018 ◽  
Vol 9 ◽  
Author(s):  
Patrícia E. de Almeida ◽  
Daniel A. M. Toledo ◽  
Gabriel S. C. Rodrigues ◽  
Heloisa D’Avila
Keyword(s):  
Prostaglandin E2 ◽  
Chagas Disease ◽  
Lipid Bodies ◽  
Escape Mechanism ◽  
Disease Impact

scholarly journals Corrigendum: Lipid Bodies as Sites of Prostaglandin E2 Synthesis During Chagas Disease: Impact in the Parasite Escape Mechanism

2018 ◽  
Vol 9 ◽  
Author(s):  
Patrícia E. de Almeida ◽  
Daniel A. M. Toledo ◽  
Gabriel S. C. Rodrigues ◽  
Heloisa D'Avila
Keyword(s):  
Prostaglandin E2 ◽  
Chagas Disease ◽  
Lipid Bodies ◽  
Escape Mechanism ◽  
Disease Impact

scholarly journals Host Cell Lipid Bodies Triggered by Trypanosoma cruzi Infection and Enhanced by the Uptake of Apoptotic Cells Are Associated With Prostaglandin E2 Generation and Increased Parasite Growth

2011 ◽  
Vol 204 (6) ◽  
pp. 951-961 ◽  
Author(s):  
Heloisa D’Avila ◽  
Célio G. Freire-de-Lima ◽  
Natalia R. Roque ◽  
Livia Teixeira ◽  
Christina Barja-Fidalgo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Prostaglandin E2 ◽  
Host Cell ◽  
Parasite Growth ◽  
Apoptotic Cells ◽  
Lipid Bodies ◽  
Cell Lipid ◽  
Cruzi Infection

scholarly journals Drug Discovery for Chagas Disease: Impact of Different Host Cell Lines on Assay Performance and Hit Compound Selection

2019 ◽  
Vol 4 (2) ◽  
pp. 82 ◽  
Author(s):  
Caio Haddad Franco ◽  
Laura Maria Alcântara ◽  
Eric Chatelain ◽  
Lucio Freitas-Junior ◽  
Carolina Borsoi Moraes
Keyword(s):  
Drug Discovery ◽  
Host Cell ◽  
Cell Lines ◽  
Chagas Disease ◽  
Host Cells ◽  
Mammalian Cell Lines ◽  
Starting Point ◽  
Disease Impact ◽  
Screening Assays ◽  
Host Parasite

Cell-based screening has become the major compound interrogation strategy in Chagas disease drug discovery. Several different cell lines have been deployed as host cells in screening assays. However, host cell characteristics and host-parasite interactions may play an important role when assessing anti-T. cruzi compound activity, ultimately impacting on hit discovery. To verify this hypothesis, four distinct mammalian cell lines (U2OS, THP-1, Vero and L6) were used as T. cruzi host cells in High Content Screening assays. Rates of infection varied greatly between different host cells. Susceptibility to benznidazole also varied, depending on the host cell and parasite strain. A library of 1,280 compounds was screened against the four different cell lines infected with T. cruzi, resulting in the selection of a total of 82 distinct compounds as hits. From these, only two hits were common to all four cell lines assays (2.4%) and 51 were exclusively selected from a single assay (62.2%). Infected U2OS cells were the most sensitive assay, as 55 compounds in total were identified as hits; infected THP-1 yielded the lowest hit rates, with only 16 hit compounds. Of the selected hits, compound FPL64176 presented selective anti-T. cruzi activity and could serve as a starting point for the discovery of new anti-chagasic drugs.

scholarly journals Lipid Bodies Are Reservoirs of Cyclooxygenase-2 and Sites of Prostaglandin-E2 Synthesis in Colon Cancer Cells

2008 ◽  
Vol 68 (6) ◽  
pp. 1732-1740 ◽  
Author(s):  
Maria T. Accioly ◽  
Patricia Pacheco ◽  
Clarissa M. Maya-Monteiro ◽  
Nina Carrossini ◽  
Bruno K. Robbs ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Prostaglandin E2 ◽  
Cancer Cells ◽  
Cyclooxygenase 2 ◽  
Lipid Bodies ◽  
Colon Cancer Cells

scholarly journals Lipid Bodies: Inflammatory Organelles Implicated in Host-Trypanosoma cruziInterplay during Innate Immune Responses

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Heloisa D'Avila ◽  
Daniel A. M. Toledo ◽  
Rossana C. N. Melo
Keyword(s):  
Chagas Disease ◽  
Current Knowledge ◽  
Public Health Issue ◽  
Lipid Bodies ◽  
Monocyte Differentiation ◽  
Host Interactions ◽  
Significant Public Health ◽  
Strong Inflammatory Response ◽  
Inflammatory Macrophages ◽  
Acute Chagas Disease

The flagellated protozoaTrypanosoma cruziis the causal agent of Chagas' disease, a significant public health issue and still a major cause of morbidity and mortality in Latin America. Acute Chagas' disease elicits a strong inflammatory response. In order to control the parasite multiplication, cells of the monocytic lineage are highly mobilized. Monocyte differentiation leads to the formation of phagocytosing macrophages, which are strongly activated and direct host defense. A distinguishing feature of Chagas' disease-triggered macrophages is the presence of increased numbers of distinct cytoplasmic organelles termed lipid bodies or lipid droplets. These organelles are actively formed in response to the parasite and are sites for synthesis and storage of inflammatory mediators. This review covers current knowledge on lipid bodies elicited by the acute Chagas' disease within inflammatory macrophages and discusses the role of these organelles in inflammation. The increased knowledge of lipid bodies in pathogenic mechanisms of infections may not only contribute to the understanding of pathogen-host interactions but may also identify new targets for intervention.

706 B-type natriuretic peptide (BNP) and ventricular arrhythmias in Chagas disease

2007 ◽  
Vol 6 (1) ◽  
pp. 155-156
Author(s):  
J MARQUES ◽  
A KANSKY ◽  
I MENDOZA ◽  
L LOPEZGOMEZ ◽  
F MOLEIRO ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Chagas Disease ◽  
Ventricular Arrhythmias

Chagas Disease After Organ Transplantation - Los Angeles, California, 2006

2006 ◽  
Author(s):  
L. Mascola ◽  
B. Kubak ◽  
S. Radhakrishna ◽  
T. Mone ◽  
R. Hunter ◽  
...  
Keyword(s):  
Los Angeles ◽  
Organ Transplantation ◽  
Chagas Disease
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