scholarly journals Insight into the Molecular Mechanism of the Transcriptional Regulation of amtB Operon in Streptomyces coelicolor

2018 ◽  
Vol 9 ◽  
Author(s):  
Zhendong Li ◽  
Xinqiang Liu ◽  
Jingzhi Wang ◽  
Ying Wang ◽  
Guosong Zheng ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Molecular Mechanism ◽  
Streptomyces Coelicolor ◽  
Insight Into

scholarly journals Crystal Structure of the Streptomyces coelicolor Sortase E1 Transpeptidase Provides Insight into the Binding Mode of the Novel Class E Sorting Signal

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0167763 ◽  
Author(s):  
Michele D. Kattke ◽  
Albert H. Chan ◽  
Andrew Duong ◽  
Danielle L. Sexton ◽  
Michael R. Sawaya ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Streptomyces Coelicolor ◽  
Binding Mode ◽  
The Novel ◽  
Sorting Signal ◽  
Insight Into ◽  
Class E

Insight into the molecular mechanism of a herbal injection by integrating network pharmacology and in vitro

2015 ◽  
Vol 173 ◽  
pp. 91-99 ◽  
Author(s):  
Yi-min Ma ◽  
Xin-zhuang Zhang ◽  
Zhen-zhen Su ◽  
Na Li ◽  
Liang Cao ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Network Pharmacology ◽  
Insight Into

Quantitative proteomic analysis of global effect of LLL12 on U87 cell's proteome: An insight into the molecular mechanism of LLL12

2015 ◽  
Vol 113 ◽  
pp. 127-142 ◽  
Author(s):  
Rekha Jain ◽  
Prajakta Kulkarni ◽  
Snigdha Dhali ◽  
Srikanth Rapole ◽  
Sanjeeva Srivastava
Keyword(s):  
Molecular Mechanism ◽  
Proteomic Analysis ◽  
Global Effect ◽  
Quantitative Proteomic Analysis ◽  
Insight Into

scholarly journals Insight into the molecular mechanism of the multitasking kinesin-8 motor

2010 ◽  
Vol 29 (20) ◽  
pp. 3437-3447 ◽  
Author(s):  
Carsten Peters ◽  
Katjuša Brejc ◽  
Lisa Belmont ◽  
Andrew J Bodey ◽  
Yan Lee ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Insight Into

scholarly journals Regulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria

2018 ◽  
Vol 200 (8) ◽  
Author(s):  
Yang Zhang ◽  
Jun Yang ◽  
Guangchun Bai
Keyword(s):  
Transcriptional Regulation ◽  
Adaptive Immunity ◽  
Parent Strain ◽  
Tuberculosis Complex ◽  
Content Type ◽  
Multifunctional Protein ◽  
Type Iii ◽  
Associated Proteins ◽  
Cyclic Dinucleotides ◽  
Insight Into

ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins (Cas) provide bacteria and archaea with adaptive immunity to specific DNA invaders. Mycobacterium tuberculosis encodes a type III CRISPR-Cas system that has not been experimentally explored. In this study, we found that the CRISPR-Cas systems of both M. tuberculosis and Mycobacterium bovis BCG were highly upregulated by deletion of Rv2837c ( cnpB ), which encodes a multifunctional protein that hydrolyzes cyclic di-AMP (c-di-AMP), cyclic di-GMP (c-di-GMP), and nanoRNAs (short oligonucleotides of 5 or fewer residues). By using genetic and biochemical approaches, we demonstrated that the CnpB-controlled transcriptional regulation of the CRISPR-Cas system is mediated by an Orn-like activity rather than by hydrolyzing the cyclic dinucleotides. Additionally, our results revealed that tuberculosis (TB) complex mycobacteria are functional in processing CRISPR RNAs (crRNAs), which are also more abundant in the Δ cnpB strain than in the parent strain. The elevated crRNA levels in the Δ cnpB strain could be partially reduced by expressing Escherichia coli orn . Our findings provide new insight into transcriptional regulation of bacterial CRISPR-Cas systems. IMPORTANCE Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins (Cas) provide adaptive immunity to specific DNA invaders. M. tuberculosis encodes a type III CRISPR-Cas system that has not been experimentally explored. In this study, we first demonstrated that the CRISPR-Cas systems in tuberculosis (TB) complex mycobacteria are functional in processing CRISPR RNAs (crRNAs). We also showed that Rv2837c (CnpB) controls the expression of the CRISPR-Cas systems in TB complex mycobacteria through an oligoribonuclease (Orn)-like activity, which is very likely mediated by nanoRNA. Since little is known about regulation of CRISPR-Cas systems, our findings provide new insight into transcriptional regulation of bacterial CRISPR-Cas systems.

New insight into lignin aggregation guiding efficient synthesis and functionalization of a lignin nanosphere with excellent performance

2021 ◽  
Author(s):  
Liheng Chen ◽  
Si-Man Luo ◽  
Cong-Min Huo ◽  
Yun-Feng Shi ◽  
Jun Feng ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Efficient Synthesis ◽  
Excellent Performance ◽  
Biomass Valorization ◽  
Insight Into

Understanding the molecular mechanism of lignin nanoparticle (LNP) formation will precisely instruct its functionalization, which is of importance for biomass valorization.

scholarly journals Cys2/His2 Zinc-Finger Proteins in Transcriptional Regulation of Flower Development

2018 ◽  
Vol 19 (9) ◽  
pp. 2589 ◽  
Author(s):  
Tianqi Lyu ◽  
Jiashu Cao
Keyword(s):  
Transcriptional Regulation ◽  
Molecular Mechanism ◽  
Zinc Finger ◽  
Flower Development ◽  
Hormonal Regulation ◽  
Regulatory Networks ◽  
Floral Organ ◽  
Zinc Finger Proteins ◽  
Higher Plant ◽  
Flowering Induction

Flower development is the core of higher-plant ontogenesis and is controlled by complex gene regulatory networks. Cys2/His2 zinc-finger proteins (C2H2-ZFPs) constitute one of the largest transcription factor families and are highly involved in transcriptional regulation of flowering induction, floral organ morphogenesis, and pollen and pistil maturation. Nevertheless, the molecular mechanism of C2H2-ZFPs has been gradually revealed only in recent years. During flowering induction, C2H2-ZFPs can modify the chromatin of FLOWERING LOCUS C, thereby providing additional insights into the quantification of transcriptional regulation caused by chromatin regulation. C2H2-ZFPs are involved in cell division and proliferation in floral organ development and are associated with hormonal regulation, thereby revealing how a flower is partitioned into four developmentally distinct whorls. The studies reviewed in this work integrate the information from the endogenous, hormonal, and environmental regulation of flower development. The structure of C2H2-ZFPs determines their function as transcriptional regulators. The findings indicate that C2H2-ZFPs play a crucial role in flower development. In this review, we summarize the current understanding of the structure, expression, and function of C2H2-ZFPs and discuss their molecular mechanism in flower development.

Microbially Originated Polyhydroxyalkanoate (PHA) Biopolymers: An Insight into the Molecular Mechanism and Biogenesis of PHA Granules

2018 ◽  
pp. 355-398 ◽  
Author(s):  
Akhilesh Kumar Singh ◽  
Laxuman Sharma ◽  
Janmejai Kumar Srivastava ◽  
Nirupama Mallick ◽  
Mohammad Israil Ansari
Keyword(s):  
Molecular Mechanism ◽  
Insight Into
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