scholarly journals A Pilin Region Affecting Host Range of the Pseudomonas aeruginosa RNA Phage, PP7

2018 ◽  
Vol 9 ◽  
Author(s):  
Eun Sook Kim ◽  
Hee-Won Bae ◽  
You-Hee Cho
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Rna Phage

scholarly journals Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy PBAD-Controlled Gene Expression in Gram-Negative Bacteria

2012 ◽  
Vol 79 (2) ◽  
pp. 718-721 ◽  
Author(s):  
F. Heath Damron ◽  
Elizabeth S. McKenney ◽  
Herbert P. Schweizer ◽  
Joanna B. Goldberg
Keyword(s):  
Gene Expression ◽  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Single Copy ◽  
Broad Host Range ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Delivery Vector ◽  
Inducible Gene

ABSTRACTWe describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the PBADpromoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, usingPseudomonas aeruginosaas the model host.

The nature of Pseudomonas aeruginosa strain PAO bacteriophage receptors

1977 ◽  
Vol 23 (6) ◽  
pp. 653-658 ◽  
Author(s):  
Andrew M. Kropinski ◽  
Lora Chan ◽  
Ken Jarrell ◽  
F. H. Milazzo
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Theoretical Approach ◽  
Rate Constants ◽  
Cell Interactions ◽  
Adsorption Rate

Receptors for phages specific to Pseudomonas aeruginosa strain PAO were studied. Phages 16, 44, 109, F8, and PBI are lipopolysaccharide (LPS) specific as shown by neutralization tests. The PhI50's of the LPS, adsorption rate constants with strain PAO and the plaque morphologies of these five phages were quite similar. Phages 1214 and 7 also appear to be LPS-specific on the basis of host–range studies. Phage 73 is pilus-specific, while phages 21 and 68 fall into a group which does not attach to pili, flagella, or LPS. A theoretical approach to the interpretation of phage–cell interactions is presented.

scholarly journals Analysis of Susceptibility Patterns of Pseudomonas aeruginosa and Isolation, Characterization of Lytic Bacteriophages Targeting Multi Drug Resistant Pseudomonas aeruginosa

2018 ◽  
Vol 11 (2) ◽  
pp. 1105-1117 ◽  
Author(s):  
Shri Natrajan Arumugam ◽  
Akarsh Chickamagalur Rudraradhya ◽  
Sathish Sadagopan ◽  
Sunilkumar Sukumaran ◽  
Ganesh Sambasivam ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Polymyxin B ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Dilution Method ◽  
Drug Resistant ◽  
Susceptibility Pattern ◽  
Infectivity Rate

Pseudomonas aeruginosa is known to be a major cause of Hospital Acquired Infections leading to high mortality in immune-compromised patients. Due to precipitous rise in antibiotic resistance, bacteriophages are significant alternative therapeutic approach for treatment and to combat resistance development. Objective of the current study was to identify MDR Pseudomonas aeruginosa from clinical isolates and to isolate bacteriophages from sewage samples against these MDR Pseudomonas aeruginosa strains. One hundred and forty-four Pseudomonas isolates were tested for their susceptibility pattern with 13 different antibiotics by micro-broth dilution method. Frequency of multidrug resistant (MDR) and Extensive Drug resistant (XDR) of Pseudomonas aeruginosa were found to be 35.5% and 23.6%, respectively. 7.61% isolates were identified as Pan drug resistant (PDR). Rate of susceptibility pattern were Piperacillin/Tazobactam 75%, Polymyxin B 74.6%, Meropenem 73.6%, Colistin 69.2%, Cefepime 54.9%, Ciprofloxacin 54.2%, Gentamicin 54.2%, Aztreonam 53.5%, Tobramycin 47.9%, Ticarcillin/Clavulanic acid 46.9%, Ertapenem 45.8%, Ceftazidime 40.3% and Imipenem 39.2%. Ninety-four bacteriophages were isolated from sewage samples against Pseudomonas aeruginosa PAO1/ATCC9027/clinical strains and host range testing study was carried out with all MDR clinical isolates. Among 51 MDR strains 34 strains were infected by phages. Phage infectivity rate were calculated for individual phages based on their host range infectivity results. AP025 and AP006 phages exhibited good infectivity rate of 39% and 30% respectively against MDR strains. Combination of 5 phages (AP002, AP006, AP011, AP025 and AP067) lysed 62.7% of the strains. Based on the obtained results, phages could be employed for treatment of infections caused by MDR strains with substantiated in-vivo experiments.

scholarly journals Transfer-deficient mutants of the narrow-host-range plasmid R91-5 of Pseudomonas aeruginosa.

1978 ◽  
Vol 135 (3) ◽  
pp. 911-919 ◽  
Author(s):  
J M Carrigan ◽  
Z M Helman ◽  
V Krishnapillai
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Narrow Host Range

scholarly journals Complete Genome Sequences of Broad-Host-Range Pseudomonas aeruginosa Bacteriophages ΦR18 and ΦS12-1

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Takaaki Furusawa ◽  
Hidetomo Iwano ◽  
Hidetoshi Higuchi ◽  
Masaru Usui ◽  
Fumito Maruyama ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Complete Genome ◽  
Broad Host Range ◽  
Bacteriophage Therapy ◽  
Genome Sequences ◽  
Prevention And Treatment

Pseudomonas aeruginosa is an important cause of racehorse keratitis. Bacteriophage therapy has the potential to aid in the prevention and treatment of diseases caused by P. aeruginosa . We present here the complete genome sequences of two phages, ΦR18 and ΦS12-1, which exhibit infectivity for a broad range of P. aeruginosa isolates.

Maintenance of broad host range plasmid RK2 replicons in Pseudomonas aeruginosa

Nature ◽  
1982 ◽  
Vol 298 (5875) ◽  
pp. 674-676 ◽  
Author(s):  
Christopher M. Thomas ◽  
Atta A. K. Hussain ◽  
Christopher A. Smith
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Plasmid Rk2

Further properties of P-2 R-factors of Pseudomonas aeruginosa and their relationship to other plasmid groups

1975 ◽  
Vol 21 (5) ◽  
pp. 592-605 ◽  
Author(s):  
M. S. Shahrabadi ◽  
L. E. Bryan ◽  
H. M. Van Den Elzen
Keyword(s):  
Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Dna Hybridization ◽  
Classification Scheme ◽  
Incompatibility Group ◽  
R Factor ◽  
Significant Homology ◽  
Pseudomonas Species ◽  
R Factors

R-factors of the P-2 (prototype R-factor R931) incompatibility group pf plasmids detected in Pseudomonas are compatible with group P, C, W, and N R-factors which are plasmids that can be transferred to Pseudomonas aeruginosa recipients. Members of the P-2 group (R130, R931) have significant homology by DNA–DNA hybridization. R-factors of the P-group (RP1, RP9) and F-group (R1) exhibited homology with P-2 R-factors but to a lesser extent than R130 with R931. Members of the 1, C, and W groups showed no significant homology with P-2 R-factors. Minicircular DNA of strain 931(R931) was not homologous with R931 DNA. The host range of R931 and R130 is limited mainly to certain Pseudomonas species including P. aeruginosa, P. fluorescens, P. putida, and P. stutzeri. These R-factors could not be transferred at detectable frequencies to any member of the Enterobacteriaceae examined. R-factor-specified pili were strongly suggested by the detection of pili by electron microscopy in R+ but not R− non-piliated mutants of P. aeruginosa strain PA01. The combined properties of R-factors 931 and similar R-factors reported before and in this study strongly support our previous contention that this group of R-factors form a significant new group of plasmids. A classification scheme previously proposed for plasmids occurring in Pseudomonas has been modified and four groups have been specified.

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