scholarly journals The Dynamics of Plasma Membrane, Metabolism and Respiration (PM-M-R) in Penicillium ochrochloron CBS 123824 in Response to Different Nutrient Limitations—A Multi-level Approach to Study Organic Acid Excretion in Filamentous Fungi

2017 ◽  
Vol 8 ◽  
Author(s):  
Pamela Vrabl ◽  
Christoph W. Schinagl ◽  
Desirée J. Artmann ◽  
Anja Krüger ◽  
Markus Ganzera ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Organic Acid ◽  
Filamentous Fungi ◽  
Acid Excretion ◽  
Nutrient Limitations ◽  
Multi Level

scholarly journals Challenging the charge balance hypothesis: reconsidering buffer effect and reuptake of previously excreted organic acids by Penicillium ochrochloron

2020 ◽  
Vol 367 (4) ◽  
Author(s):  
D J Artmann ◽  
P Vrabl ◽  
R Gianordoli ◽  
W Burgstaller
Keyword(s):  
Zinc Oxide ◽  
Plasma Membrane ◽  
Organic Acids ◽  
Organic Acid ◽  
Growth Parameters ◽  
Facilitated Diffusion ◽  
Acid Excretion ◽  
Buffer Effect ◽  
Charge Balance ◽  
Balance Hypothesis

ABSTRACT Penicillium ochrochloron was used in the past for the leaching of zinc from a zinc oxide containing filter dust via excreted organic acids. Organic acid excretion by P. ochrochloron was stimulated by the addition of an extracellular buffer (2-(N-Morpholino)ethanesulfonic acid, MES; or zinc oxide, ZnO: ZnO + 2 H+ → Zn2+ + H2O). It was tested if the buffer stimulated excretion of organic acid anions is due to the necessity of an anion efflux across the plasma membrane to maintain electroneutrality by balancing the excretion of protons by the H+-ATPase. This charge balance hypothesis was previously postulated for P. ochrochloron. Two strains of P. ochrochloron were studied, which differed in growth parameters and amount of excreted organic acids. From the results, it was concluded that charge balance at the plasma membrane is not the main reason for organic acid excretion in these two strains of P. ochrochloron. Furthermore, the phenomenon of reuptake of excreted organic acids in the presence of about 100 mM of glucose is confirmed. It is suggested that the equilibrium between extracellular and intracellular organic acid anions may be maintained passively by a facilitated diffusion transporter.

Carnitine Metabolism and Urinary Organic Acid Excretion in Reye's Syndrome and Salicylate Intoxication.

1990 ◽  
Vol 32 (4) ◽  
pp. 406-409 ◽  
Author(s):  
Masaki Takayanagi ◽  
Hiroaki Kakinuma ◽  
Shigenori Yamamoto ◽  
Hironori Nakajima
Keyword(s):  
Organic Acid ◽  
Acid Excretion ◽  
Urinary Organic Acid ◽  
Reye's Syndrome ◽  
Carnitine Metabolism ◽  
Salicylate Intoxication

scholarly journals Organic Acid Excretion in Penicillium ochrochloron Increases with Ambient pH

2012 ◽  
Vol 3 ◽  
Author(s):  
Pamela Vrabl ◽  
Viktoria Fuchs ◽  
Barbara Pichler ◽  
Christoph W. Schinagl ◽  
Wolfgang Burgstaller
Keyword(s):  
Organic Acid ◽  
Acid Excretion

scholarly journals Functional Analysis of Sterol Transporter Orthologues in the Filamentous Fungus Aspergillus nidulans

2015 ◽  
Vol 14 (9) ◽  
pp. 908-921 ◽  
Author(s):  
Nicole Bühler ◽  
Daisuke Hagiwara ◽  
Norio Takeshita
Keyword(s):  
Plasma Membrane ◽  
Aspergillus Nidulans ◽  
Filamentous Fungi ◽  
Gene Deletion ◽  
Fluorescent Protein ◽  
Fungal Growth ◽  
Hyphal Growth ◽  
Apical Plasma Membrane ◽  
Important Species ◽  
Content Type

ABSTRACT Polarized growth in filamentous fungi needs a continuous supply of proteins and lipids to the growing hyphal tip. One of the important membrane compounds in fungi is ergosterol. At the apical plasma membrane ergosterol accumulations, which are called sterol-rich plasma membrane domains (SRDs). The exact roles and formation mechanism of the SRDs remained unclear, although the importance has been recognized for hyphal growth. Transport of ergosterol to hyphal tips is thought to be important for the organization of the SRDs. Oxysterol binding proteins, which are conserved from yeast to human, are involved in nonvesicular sterol transport. In Saccharomyces cerevisiae seven oxysterol-binding protein homologues (OSH1 to -7) play a role in ergosterol distribution between closely located membranes independent of vesicle transport. We found five homologous genes ( oshA to oshE ) in the filamentous fungi Aspergillus nidulans . The functions of OshA-E were characterized by gene deletion and subcellular localization. Each gene-deletion strain showed characteristic phenotypes and different sensitivities to ergosterol-associated drugs. Green fluorescent protein-tagged Osh proteins showed specific localization in the late Golgi compartments, puncta associated with the endoplasmic reticulum, or diffusely in the cytoplasm. The genes expression and regulation were investigated in a medically important species Aspergillus fumigatus , as well as A. nidulans . Our results suggest that each Osh protein plays a role in ergosterol distribution at distinct sites and contributes to proper fungal growth.

Organic acid excretion in a patient with 3-hydroxy-3-methylglutaryl-Coa lyase deficiency: Facts and artefacts

1978 ◽  
Vol 90 (2) ◽  
pp. 187-193 ◽  
Author(s):  
M. Duran ◽  
D. Retting ◽  
S.K. Wadman ◽  
C. Jakobs ◽  
R.B.H. Schutgens ◽  
...  
Keyword(s):  
Organic Acid ◽  
Acid Excretion ◽  
Lyase Deficiency

V-ATPases of the plasma membrane.

1992 ◽  
Vol 172 (1) ◽  
pp. 29-37 ◽  
Author(s):  
S Gluck
Keyword(s):  
Plasma Membrane ◽  
Acid Excretion ◽  
Electrophysiological Properties

V-ATPases reside in high densities on the plasma membrane in specialized types of insect and vertebrate cells. They provide unique biochemical and electrophysiological properties that allow them to function in energizing the plasma membrane in insects, and in cellular acid excretion in vertebrates.

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