scholarly journals Evaluation of Recombinant Multi-Epitope Outer Membrane Protein-Based Klebsiella pneumoniae Subunit Vaccine in Mouse Model

2017 ◽  
Vol 8 ◽  
Author(s):  
Litty Babu ◽  
Siva R. Uppalapati ◽  
Murali H. Sripathy ◽  
Prakash N. Reddy
Keyword(s):  
Membrane Protein ◽  
Klebsiella Pneumoniae ◽  
Mouse Model ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Subunit Vaccine

scholarly journals A Recombinant Chlamydia trachomatis MOMP Vaccine Elicits Cross-serogroup Protection in Mice Against Vaginal Shedding and Infertility

2019 ◽  
Vol 221 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Delia F Tifrea ◽  
Sukumar Pal ◽  
Luis M de la Maza
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Immune Responses ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Subunit Vaccine ◽  
Bacterial Pathogen ◽  
Male Mice ◽  
Fertility Rates ◽  
Sexually Transmitted

Abstract Background Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen worldwide. Here, we determined the ability of a C. trachomatis recombinant major outer membrane protein (rMOMP) vaccine to elicit cross-serogroup protection. Methods Female C3H/HeN mice were vaccinated by mucosal and systemic routes with C. trachomatis serovar D (UW-3/Cx) rMOMP and challenged in the ovarian bursa with serovars D (UW-3/Cx), D (UCI-96/Cx), E (IOL-43), or F (N.I.1). CpG-1826 and Montanide ISA 720 were used as adjuvants. Results Immune responses following vaccination were more robust against the most closely related serovars. Following a genital challenge (as determined by number of mice with positive vaginal cultures, number of positive cultures, number of inclusion forming units recovered, and number of days with positive cultures) mice challenged with C. trachomatis serovars of the same complex were protected but not those challenged with serovar F (N.I.1) from a different subcomplex. Females were caged with male mice. Based on fertility rates, number of embryos, and hydrosalpinx formation, vaccinated mice were protected against challenges with serovars D (UW-3/Cx), D (UCI-96/Cx), and E (IOL-43) but not F (N.I.1). Conclusions This is the first subunit vaccine shown to protect mice against infection, pathology, and infertility caused by different C. trachomatis serovars.

scholarly journals Enhancement of Pulmonary Clearance ofMoraxella (Branhamella) catarrhalisfollowing Immunization with Outer Membrane Protein CD in a Mouse Model

1998 ◽  
Vol 178 (6) ◽  
pp. 1667-1675 ◽  
Author(s):  
Timothy F. Murphy ◽  
Jennelle M. Kyd ◽  
Anna John ◽  
Charmaine Kirkham ◽  
Allan W. Cripps
Keyword(s):  
Membrane Protein ◽  
Mouse Model ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Pulmonary Clearance

scholarly journals In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae

2005 ◽  
Vol 49 (8) ◽  
pp. 3562-3565 ◽  
Author(s):  
Philippe Bidet ◽  
Béatrice Burghoffer ◽  
Valérie Gautier ◽  
Naïma Brahimi ◽  
Patricia Mariani-Kurkdjian ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Klebsiella Pneumoniae ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Major Outer Membrane Protein ◽  
In Vivo Selection ◽  
Selection Of

ABSTRACT We describe in vivo selection of a Klebsiella pneumoniae strain with diminished imipenem susceptibility attributable to plasmid-encoded ACC-1 β-lactamase production and loss of a 36-kDa major outer membrane protein, together with transfer of this plasmid from K. pneumoniae to Escherichia coli in a Tunisian infant.

Recombinant outer membrane protein 25c from Brucella abortus induces Th1 and Th2 mediated protection against Brucella abortus infection in mouse model

2018 ◽  
Vol 99 ◽  
pp. 9-18 ◽  
Author(s):  
Soumya Paul ◽  
Bhavani V. Peddayelachagiri ◽  
Sowmya Nagaraj ◽  
Joseph J. Kingston ◽  
Harsh V. Batra
Keyword(s):  
Membrane Protein ◽  
Mouse Model ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Brucella Abortus ◽  
Th1 And Th2
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