scholarly journals The Neurospora crassa PP2A Regulatory Subunits RGB1 and B56 Are Required for Proper Growth and Development and Interact with the NDR Kinase COT1

2017 ◽  
Vol 8 ◽  
Author(s):  
Hila Shomin-Levi ◽  
Oded Yarden
Keyword(s):  
Neurospora Crassa ◽  
Growth And Development ◽  
Regulatory Subunits ◽  
Ndr Kinase

scholarly journals Neurospora crassa Protein Arginine Methyl Transferases Are Involved in Growth and Development and Interact with the NDR Kinase COT1

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e80756 ◽  
Author(s):  
Daria Feldman ◽  
Carmit Ziv ◽  
Rena Gorovits ◽  
Michal Efrat ◽  
Oded Yarden
Keyword(s):  
Neurospora Crassa ◽  
Growth And Development ◽  
Ndr Kinase

scholarly journals Methylation of Histone H3 Lysine 36 Is Required for Normal Development in Neurospora crassa

2005 ◽  
Vol 4 (8) ◽  
pp. 1455-1464 ◽  
Author(s):  
Keyur K. Adhvaryu ◽  
Stephanie A. Morris ◽  
Brian D. Strahl ◽  
Eric U. Selker
Keyword(s):  
Neurospora Crassa ◽  
Growth And Development ◽  
Histone H3 ◽  
Normal Growth ◽  
Wild Type ◽  
Set Domain ◽  
Amino Terminal ◽  
Histone H3 Gene ◽  
Type Gene

ABSTRACT The SET domain is an evolutionarily conserved domain found predominantly in histone methyltransferases (HMTs). The Neurospora crassa genome includes nine SET domain genes (set-1 through set-9) in addition to dim-5, which encodes a histone H3 lysine 9 HMT required for DNA methylation. We demonstrate that Neurospora set-2 encodes a histone H3 lysine 36 (K36) methyltransferase and that it is essential for normal growth and development. We used repeat induced point mutation to make a set-2 mutant (set-2 RIP1 ) with multiple nonsense mutations. Western analyses revealed that the mutant lacks SET-2 protein and K36 methylation. An amino-terminal fragment that includes the AWS, SET, and post-SET domains of SET-2 proved sufficient for K36 HMT activity in vitro. Nucleosomes were better substrates than free histones. The set-2 RIP1 mutant grows slowly, conidiates poorly, and is female sterile. Introducing the wild-type gene into the mutant complemented the defects, confirming that they resulted from loss of set-2 function. We replaced the wild-type histone H3 gene (hH3) with an allele producing a Lys to Leu substitution at position 36 and found that this hH3 K36L mutant phenocopied the set-2 RIP1 mutant, confirming that the observed defects in growth and development result from inability to methylate K36 of H3. Finally, we used chromatin immunoprecipitation to demonstrate that actively transcribed genes in Neurospora crassa are enriched for H3 methylated at lysines 4 and 36. Taken together, our results suggest that methylation of K36 in Neurospora crassa is essential for normal growth and development.

GROWTH AND DEVELOPMENT OF ISOLATED HYPHAL TIPS OF NEUROSPORA CRASSA IN THE SORBITOL-CONTAINING MEDIUM

Tsitologiya ◽  
2018 ◽  
Vol 60 (1) ◽  
pp. 42-48
Author(s):  
T. V. Potapova ◽  
◽  
L. Yu. Boitzova ◽  
S. A. Golyshev ◽  
A. Ya. Dunina-Barkovskaya ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Growth And Development

scholarly journals GPR-4 Is a Predicted G-Protein-Coupled Receptor Required for Carbon Source-Dependent Asexual Growth and Development in Neurospora crassa

2006 ◽  
Vol 5 (8) ◽  
pp. 1287-1300 ◽  
Author(s):  
Liande Li ◽  
Katherine A. Borkovich
Keyword(s):  
Carbon Source ◽  
Neurospora Crassa ◽  
G Protein ◽  
Growth And Development ◽  
Carbon Sources ◽  
Wild Type ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Camp Levels ◽  
Mass Accumulation

ABSTRACT The filamentous fungus Neurospora crassa is able to utilize a wide variety of carbon sources. Here, we examine the involvement of a predicted G-protein-coupled receptor (GPCR), GPR-4, during growth and development in the presence of different carbon sources in N. crassa. Δgpr-4 mutants have reduced mass accumulation compared to the wild type when cultured on high levels of glycerol, mannitol, or arabinose. The defect is most severe on glycerol and is cell density dependent. The genetic and physical relationship between GPR-4 and the three N. crassa Gα subunits (GNA-1, GNA-2, and GNA-3) was explored. All three Gα mutants are defective in mass accumulation when cultured on glycerol. However, the phenotypes of Δgna-1 and Δgpr-4 Δgna-1 mutants are identical, introduction of a constitutively activated gna-1 allele suppresses the defects of the Δgpr-4 mutation, and the carboxy terminus of GPR-4 interacts most strongly with GNA-1 in the yeast two-hybrid assay. Although steady-state cyclic AMP (cAMP) levels are normal in Δgpr-4 strains, exogenous cAMP partially remediates the dry mass defects of Δgpr-4 mutants on glycerol medium and Δgpr-4 strains lack the transient increase in cAMP levels observed in the wild type after addition of glucose to glycerol-grown liquid cultures. Our results support the hypothesis that GPR-4 is coupled to GNA-1 in a cAMP signaling pathway that regulates the response to carbon source in N. crassa. GPR-4-related GPCRs are present in the genomes of several filamentous ascomycete fungal pathogens, raising the possibility that a similar pathway regulates carbon sensing in these organisms.

scholarly journals The NDR Kinase Scaffold HYM1/MO25 Is Essential for MAK2 MAP Kinase Signaling in Neurospora crassa

PLoS Genetics ◽  
2012 ◽  
Vol 8 (9) ◽  
pp. e1002950 ◽  
Author(s):  
Anne Dettmann ◽  
Julia Illgen ◽  
Sabine März ◽  
Timo Schürg ◽  
Andre Fleissner ◽  
...  
Keyword(s):  
Map Kinase ◽  
Neurospora Crassa ◽  
Kinase Signaling ◽  
Map Kinase Signaling ◽  
Ndr Kinase

scholarly journals Shared and Independent Roles for a Gαi Protein and Adenylyl Cyclase in Regulating Development and Stress Responses in Neurospora crassa

2002 ◽  
Vol 1 (4) ◽  
pp. 634-642 ◽  
Author(s):  
F. Douglas Ivey ◽  
Ann M. Kays ◽  
Katherine A. Borkovich
Keyword(s):  
Neurospora Crassa ◽  
Adenylyl Cyclase ◽  
Stress Responses ◽  
Growth And Development ◽  
Submerged Culture ◽  
Wild Type Strain ◽  
Wild Type ◽  
Liquid Cultures ◽  
Aerial Hyphae ◽  
Gαi Protein

ABSTRACT Growth and development are regulated using cyclic AMP (cAMP)-dependent and -independent pathways in Neurospora crassa. The cr-1 adenylyl cyclase mutant lacks detectable cAMP and exhibits numerous defects, including colonial growth habit, short aerial hyphae, premature conidiation on plates, inappropriate conidiation in submerged culture, and increased thermotolerance. Evidence suggests that the heterotrimeric Gα protein GNA-1 is a direct positive regulator of adenylyl cyclase. Δgna-1 strains are female-sterile, and Δgna-1 strains have reduced apical extension rates on normal and hyperosmotic medium, greater resistance to oxidative and heat stress, and stunted aerial hyphae compared to the wild-type strain. In this study, a Δgna-1 cr-1 double mutant was analyzed to differentiate cAMP-dependent and -independent signaling pathways regulated by GNA-1. Δgna-1 cr-1 mutants have severely restricted colonial growth and do not produce aerial hyphae on plates or in standing liquid cultures. Addition of cAMP to plates or standing liquid cultures rescues cr-1, but not Δgna-1 cr-1, defects, which is consistent with previous results demonstrating that Δgna-1 mutants do not respond to exogenous cAMP. The females of all strains carrying the Δgna-1 mutation are sterile; however, unlike cr-1 and Δgna-1 strains, the Δgna-1 cr-1 mutant does not produce protoperithecia. The Δgna-1 and cr-1 mutations were synergistic with respect to inappropriate conidiation during growth in submerged culture. Thermotolerance followed the order wild type < Δgna-1 < cr-1 = Δgna-1 cr-1, consistent with a cAMP-dependent process. Taken together, the results suggest that in general, GNA-1 and CR-1 regulate N. crassa growth and development using parallel pathways, while thermotolerance is largely dependent on cAMP.

scholarly journals Global Analysis of Serine-Threonine Protein Kinase Genes in Neurospora crassa

2011 ◽  
Vol 10 (11) ◽  
pp. 1553-1564 ◽  
Author(s):  
Gyungsoon Park ◽  
Jacqueline A. Servin ◽  
Gloria E. Turner ◽  
Lorena Altamirano ◽  
Hildur V. Colot ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Neurospora Crassa ◽  
Growth And Development ◽  
Global Analysis ◽  
Large Fraction ◽  
Kinase Gene ◽  
Content Type ◽  
Chemical Screening ◽  
Chemical Treatments ◽  
Total Percentage

ABSTRACTSerine/threonine (S/T) protein kinases are crucial components of diverse signaling pathways in eukaryotes, including the model filamentous fungusNeurospora crassa. In order to assess the importance of S/T kinases toNeurosporabiology, we embarked on a global analysis of 86 S/T kinase genes inNeurospora. We were able to isolate viable mutants for 77 of the 86 kinase genes. Of these, 57% exhibited at least one growth or developmental phenotype, with a relatively large fraction (40%) possessing a defect in more than one trait. S/T kinase knockouts were subjected to chemical screening using a panel of eight chemical treatments, with 25 mutants exhibiting sensitivity or resistance to at least one chemical. This brought the total percentage of S/T mutants with phenotypes in our study to 71%. Mutants lackingapg-1, an S/T kinase required for autophagy in other organisms, possessed the greatest number of phenotypes, with defects in asexual and sexual growth and development and in altered sensitivity to five chemical treatments. We showed that NCU02245/stk-19is required for chemotropic interactions between female and male cells during mating. Finally, we demonstrated allelism between the S/T kinase gene NCU00406 andvelvet(vel), encoding a p21-activated protein kinase (PAK) gene important for asexual and sexual growth and development inNeurospora.

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