scholarly journals Effect of Different Antibiotic Chemotherapies on Pseudomonas aeruginosa Infection In Vitro of Primary Human Corneal Fibroblast Cells

2017 ◽  
Vol 8 ◽  
Author(s):  
Maria del Mar Cendra ◽  
Myron Christodoulides ◽  
Parwez Hossain
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fibroblast Cells ◽  
Corneal Fibroblast ◽  
Pseudomonas Aeruginosa Infection

Exogenous rh-urokinase modifies inflammation and Pseudomonas aeruginosa infection in a rat chronic pulmonary infection model

1993 ◽  
Vol 39 (12) ◽  
pp. 1127-1134 ◽  
Author(s):  
David A. Hart ◽  
Francis Green ◽  
Paul Whidden ◽  
Jack Henkin ◽  
Donald E. Woods
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pulmonary Infection ◽  
Pulmonary Inflammation ◽  
Infection Model ◽  
Sprague Dawley ◽  
Short Term ◽  
Intratracheal Administration ◽  
Pseudomonas Aeruginosa Infection ◽  
Vitro Analysis

The effect of recombinant human urokinase (rh-UK) in a rat model of chronic Pseudomonas aeruginosa pulmonary infection was studied. Efficacy was assessed by lung histology and quantitative bacteriology. Male Sprague–Dawley rats received 1 × 104 or 1 × 105P. aeruginosa encapsulated in agar beads via the intratracheal route on day 1. Intratracheal administration of up to 12 500 units of rh-UK on day 21 led to a dose-dependent disappearance of viable organisms from the lungs by day 24 in rats receiving 104 organisms. In slightly longer term infections (30 days), rh-UK was still effective in facilitating the disappearance of the organisms from the lungs of most of the treated animals. rh-UK was effective in eliminating organisms when animals were infected with 104, but not 105 bacteria. In vitro analysis revealed that rh-UK was not directly toxic for the organisms. Histologically, lungs from short-term infected control animals exhibited acute inflammation, inflammatory cell infiltrates, and fibrin deposition. Histology of lungs from UK-treated, short-term infected rats revealed decreased airway inflammation and cellular infiltration compared with infected controls. Lungs from infected animals treated with 12 500 units of rh-UK were histologically indistinguishable from the lungs of uninfected control animals, except for the foreign body reaction. These results indicate that exogenous rh-UK may be efficacious in the treatment of pulmonary inflammation accompanying exposure to Gram-negative bacteria such as P. aeruginosa.Key words: chronic pulmonary infection, Pseudomonas aeruginosa infection, fibrinolysis, urokinase.

scholarly journals Macrolides protect against Pseudomonas aeruginosa infection via inhibition of inflammasomes

2017 ◽  
Vol 313 (4) ◽  
pp. L677-L686 ◽  
Author(s):  
Li-Chao Fan ◽  
Jie-Lu Lin ◽  
Jia-Wei Yang ◽  
Bei Mao ◽  
Hai-Wen Lu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lung Injury ◽  
Treatment Options ◽  
Therapeutic Effects ◽  
Bacterial Clearance ◽  
Pseudomonas Aeruginosa Infection ◽  
Related Proteins ◽  
Nlrp3 Inflammasomes ◽  
Chronic Lung Infection

Macrolides antibiotics have been effectively used in many chronic diseases, especially with Pseudomonas aeruginosa (P. aeruginosa) infection. The mechanisms underlying the therapeutic effects of macrolides in these diseases remain poorly understood. We established a mouse model of chronic lung infection using P. aeruginosa agar-beads, with azithromycin treatment or placebo. Lung injury, bacterial clearance, and inflammasome-related proteins were measured. In vitro, the inflammasomes activation induced by flagellin or ATP were assessed in LPS-primed macrophages with or without macrolides treatment. Plasma IL-18 levels were determined from patients who were diagnosed with bronchiectasis isolated with or without P. aeruginosa and treated with azithromycin for 3–5 days. Azithromycin treatment enhanced bacterial clearance and attenuated lung injury in mice chronically infected with P. aeruginosa, which resulted from the inhibition of caspase-1-dependent IL-1β and IL-18 secretion. In vitro, azithromycin and erythromycin inhibited NLRC4 and NLRP3 inflammasomes activation. Plasma IL-18 levels were higher in bronchiectasis patients with P. aeruginosa isolation compared with healthy controls. Azithromycin administration markedly decreased IL-18 secretion in bronchiectasis patients. The results of this study reveal that azithromycin and erythromycin exert a novel anti-inflammatory effect by attenuating inflammasomes activation, which suggests potential treatment options for inflammasome-related diseases.

scholarly journals Alkyl-Quinolones derivatives as potential biomarkers for Pseudomonas aeruginosa infection chronicity in Cystic Fibrosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Serge Michalet ◽  
Pierre-Marie Allard ◽  
Carine Commun ◽  
Van Thanh Nguyen Ngoc ◽  
Kodjo Nouwade ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Liquid Culture ◽  
Multivariate Analyses ◽  
In Vitro Cultures ◽  
Clinical Stages ◽  
Chronic Colonization ◽  
Pseudomonas Aeruginosa Infection ◽  
Definition Of

AbstractIn Cystic Fibrosis (CF), a rapid and standardized definition of chronic infection would allow a better management of Pseudomonas aeruginosa (Pa) infections, as well as a quick grouping of patients during clinical trials allowing better comparisons between studies. With this purpose, we compared the metabolic profiles of 44 in vitro cultures of Pa strains isolated from CF patients at different stages of infection in order to identify metabolites differentially synthetized according to these clinical stages. Compounds produced and secreted by each strain in the supernatant of a liquid culture were analysed by metabolomic approaches (UHPLC-DAD-ESI/QTOF, UV and UPLC-Orbitrap, MS). Multivariate analyses showed that first colonization strains could be differentiated from chronic colonization ones, by producing notably more Alkyl-Quinolones (AQs) derivatives. Especially, five AQs were discriminant: HQC5, HQNOC7, HQNOC7:1, db-PQS C9 and HQNOC9:1. However, the production of HHQ was equivalent between strain types. The HHQ/HQNOC9:1 ratio was then found to be significantly different between chronic and primo-colonising strains by using both UV (p = 0.003) and HRMS data (p = 1.5 × 10–5). Our study suggests that some AQ derivatives can be used as biomarkers for an improved management of CF patients as well as a better definition of the clinical stages of Pa infection.

scholarly journals Studies on Pseudomonas aeruginosa Infection in Hatcheries and Chicken

2020 ◽  
Vol 71 (1) ◽  
pp. 1953
Author(s):  
R. D. ERAKY ◽  
W. A. ABD EL-GHANY ◽  
K. M. SOLIMAN
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Abdominal Cavity ◽  
Broiler Chicks ◽  
Pathogenicity Test ◽  
Internal Organs ◽  
Highly Pathogenic ◽  
Pseudomonas Aeruginosa Infection ◽  
Polymerase Chain

The aim of this work was to spot light on the presence of Pseudomonas aeruginosa (P. aeruginosa) strains in hatcheries and dead in shell embryos. A total of 406 samples representing 200 and 206 swabs from hatcheries environment and yolk sacs of dead in shell embryos were collected from Damietta governorate, Egypt. P. aeruginosa was isolated and identified. Some virulent genes (toxA, psIA and fliC) of P. aeruginosa were detected using polymerase chain reaction (PCR). The antimicrobial susceptibility of P. aeruginosa was tested in vitro. Day and 11 days old broiler chicks were challenged with P. aeruginosa to determine the pathogenicity of the isolated strains. The results showed that P. aeruginosa was recovered from 16 (8%) out of 200 hatcheries and from 17 (8.25%) out of 206 chicken embryos samples. Isolated strains of P. aeruginosa showed presence of toxA, psIA and fliC virulent genes. P. aeruginosa strains were sensitive (100%) to ciprofloxacin, levofloxacin and gentamycin but resistant (100%) to amoxycillin/clavulanic acid, doxycycline and erythromycin. The pathogenicity test of day and 11 days old chicks revealed that P. aeruginosa was highly pathogenic induced mortality rates of 72 and 40%, respectively. Septicaemia of internal organs, unabsorbed yolk sacs, pneumonia, greenish exudates in the abdominal cavity, liver necrosis and enteritis were the predominant lesions. Histopathological changes supported the previous lesions. In conclusion, P. aeruginosa is of great importance pathogen of embryos and newly hatched chicks based on presence of virulent genes as well as in vivo pathogenicity study; respectively.

scholarly journals Mechanism of Eucalyptus Volatile Oil Application for Preventing and Treating Pseudomonas Aeruginosa Infection in Vitro

2020 ◽  
Author(s):  
Lei Yang ◽  
Qing Huang ◽  
Xiaodong Huang ◽  
Zhimin Lin ◽  
Xingxin Gao ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Volatile Oil ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Rt Pcr ◽  
Zone Of Inhibition ◽  
Biofilm Model ◽  
Concentration Test ◽  
Pseudomonas Aeruginosa Infection

Abstract Background The mechanism of eucalyptus volatile oil application on the prevention and treatment of burn infections caused by Pseudomonas aeruginosa (PA) was need to examined.Methods The effect of eucalyptus volatile oil on bacterial biofilm was investigated using a PA biofilm model. The expression of LasI mRNA in PA was detected by RT-PCR. Results The minimum inhibitory concentration test showed that the volatile oil of Eucalyptus urophylla in 20% concentration or more could exert an antibacterial effect. However, neither high nor low concentration of the eucalyptus volatile oil had a zone of inhibition. In scanning electron microscopy, the volatile oil group exhibited a significant delay compared with the control group. The volatile oil group also had a significantly lower LasI mRNA expression than the control group. Conclusion As a Chinese medicine, the volatile oil of E. urophylla can affect PA proliferation and biofilm formation by interfering with the expression of LasI, thereby successfully preventing and treating burn infection caused by PA.

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