scholarly journals The Probiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Bifidobacterium animalis subsp. lactis BPL6 Reduces Pathogen Loads and Improves Gut Health of Weaned Piglets Orally Challenged with Salmonella Typhimurium

2017 ◽  
Vol 8 ◽  
Author(s):  
Emili Barba-Vidal ◽  
Lorena Castillejos ◽  
Victor F. B. Roll ◽  
Gloria Cifuentes-Orjuela ◽  
José A. Moreno Muñoz ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Bifidobacterium Longum ◽  
Gut Health ◽  
Weaned Piglets ◽  
Bifidobacterium Animalis

scholarly journals Using Nutritional Strategies to Shape the Gastro-Intestinal Tracts of Suckling and Weaned Piglets

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 402
Author(s):  
Anne M.S. Huting ◽  
Anouschka Middelkoop ◽  
Xiaonan Guan ◽  
Francesc Molist
Keyword(s):  
Future Research ◽  
Gut Health ◽  
Weaned Piglets ◽  
Functional Ingredients ◽  
Progressive Development ◽  
Creep Feed ◽  
Fermentation Capacity ◽  
Gastric Barrier ◽  
Gastro Intestinal Tract ◽  
Nutritional Strategies

This is a comprehensive review on the use of nutritional strategies to shape the functioning of the gastro-intestinal tract in suckling and weaned piglets. The progressive development of a piglet’s gut and the associated microbiota and immune system offers a unique window of opportunity for supporting gut health through dietary modulation. This is particularly relevant for large litters, for which sow colostrum and milk are insufficient. The authors have therefore proposed the use of supplemental milk and creep feed with a dual purpose. In addition to providing nutrients to piglets, supplemental milk can also serve as a gut modulator in early life by incorporating functional ingredients with potential long-term benefits. To prepare piglets for weaning, it is important to stimulate the intake of solid feed before weaning, in addition to stimulating the number of piglets eating. The use of functional ingredients in creep feed and a transition diet around the time of weaning helps to habituate piglets to solid feed in general, while also preparing the gut for the digestion and fermentation of specific ingredients. In the first days after weaning (i.e., the acute phase), it is important to maintain high levels of feed intake and focus on nutritional strategies that support good gastric (barrier) function and that avoid overloading the impaired digestion and fermentation capacity of the piglets. In the subsequent maturation phase, the ratio of lysine to energy can be increased gradually in order to stimulate piglet growth. This is because the digestive and fermentation capacity of the piglets is more mature at this stage, thus allowing the inclusion of more fermentable fibres. Taken together, the nutritional strategies addressed in this review provide a structured approach to preparing piglets for success during weaning and the period that follows. The implementation of this approach and the insights to be developed through future research can help to achieve some of the most important goals in pig production: reducing piglet mortality, morbidity and antimicrobial use.

scholarly journals Wheat bran fermented by mixed fungal strains improves the digestibility of crude fiber and may benefit the gut health without impacting the growth performance in weaned pigs

2021 ◽  
Vol 12 (7) ◽  
pp. 2962-2971
Author(s):  
Yuheng Luo ◽  
Jun He ◽  
Hua Li ◽  
Cong Lan ◽  
Jingyi Cai ◽  
...  
Keyword(s):  
Growth Performance ◽  
Wheat Bran ◽  
Nutrient Digestibility ◽  
Crude Fiber ◽  
Gut Health ◽  
Intestinal Health ◽  
Weaned Piglets ◽  
Weaned Pigs ◽  
Fungal Strains

This study was conducted to compare the effect of raw (WB) or mixed fungi-fermented wheat bran (FWB) on the growth, nutrient digestibility and intestinal health in weaned piglets.

scholarly journals Identification, Characterization, and Antioxidant Potential of Bifidobacterium longum subsp. longum Strains Isolated From Feces of Healthy Infants

2021 ◽  
Vol 12 ◽  
Author(s):  
Li Zhao ◽  
Song Wang ◽  
Jiahuan Dong ◽  
Jialu Shi ◽  
Jiaqi Guan ◽  
...  
Keyword(s):  
Infant Nutrition ◽  
Bifidobacterium Longum ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Heat Shock Protein 60 ◽  
Intestinal Epithelial ◽  
Intact Cells ◽  
Bifidobacterium Animalis ◽  
Healthy Infants

Increasing evidence has indicated that oxidative stress is associated with the health of infants. Bifidobacterium, especially B. longum subsp. longum strains, are abundant in the gut microbiota of infants, which may have the potential to ameliorate oxidative damage. Thus, this study aimed to isolate and screen B. longum subsp. longum strains with probiotic characters and antioxidant properties as infants’ dietary supplements. In this study, 24 B. longum subsp. longum strains were isolated from 15 healthy infants identified via 16S rRNA and heat shock protein 60 (hsp60) sequences. B. longum subsp. longum B13, F2, K4, K5, K10, K13, and K15 strains were selected based on high values obtained from autoaggregation, hydrophobicity, and adhesion assays to HT-29 cells. Among these seven strains, B. longum subsp. longum F2, K5, K10, and K15 were selected according to the high tolerance of gastrointestinal tract conditions compared to Bifidobacterium animalis subsp. lactis BB-12. Among these four strains, B. longum subsp. longum K5 was susceptible to common antibiotics and showed the highest intestinal epithelial cell proliferation of CCD 841 CoN. Additionally, B. longum subsp. longum K5 showed a strong antioxidant capacity, and its supernatant exhibited better activity of reducing power, hydroxyl radical scavenging, and DPPH radical scavenging than that of the intact cells with cell-free extracts. The findings indicated that B. longum subsp. longum K5 could be used as a probiotic candidate in infant nutrition.

scholarly journals 398 Towards new feeding approaches for optimizing nutritional status, immunity and host-microbiota interactions in neonatal and weaned piglets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 181-181
Author(s):  
Martin Lessard ◽  
Mylène Blais ◽  
Guylaine Talbot ◽  
J Jacques Matte ◽  
Ann Letellier ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Intestinal Microbiota ◽  
System Development ◽  
Feed Additives ◽  
Epithelial Cell Line ◽  
Host Immune System ◽  
Gut Health ◽  
Weaned Piglets ◽  
Immune System Development

Abstract Lactation, feeding conditions, microbial interventions and piglet growth in the first few weeks of life have important impact on the intestinal microbiota establishment and immune system development of piglets. Indeed, colostrum and milk contain various bioactive components such as immune factors, antimicrobial peptides and oligosaccharides that contribute to maintain intestinal homeostasis and regulate interactions between microbiota and host immune system. Recent results revealed that low birth weight piglet (LBWP) with poor weight gain during the first two weeks of life develop different intestinal microbiota and immune response profiles compared to high BWP (HBWP) littermates. Consequently, piglets within litters may have different resilience to infections after weaning and benefit from feed additives in a specific manner. A study has been performed to evaluate the potential of bovine colostrum extract (BC) as replacement to plasma proteins for improving gut health and resilience to Salmonella infection in piglets. Results revealed that in weaned piglets fed BC, intestinal microbiota was differently modulated and bacterial dysbiosis induced by Salmonella was restored faster. Moreover, expression of genes involved in innate immunity such as β-defensin-2 and glutathione peroxidase-2 was respectively down- and up-regulated in BC fed piglets. A combination of dietary supplementation with BC, cupper and vitamins A and D has also been tested in LBWP and HBWP, and there is clear evidence that BC in combination with other feed additives promote growth and gut health in both LBWP and HBWP. The porcine intestinal epithelial cell line IPEC-J2 was used to better understand the functional properties of BC. Results indicated that BC improves wound healing, enhances barrier function and modulates the expression of several genes involved in innate immune response. Finally, as microbial intervention, the potential of fecal transplantation to modulate intestinal microbiota and immune system development of piglets is under investigation and will be discussed.

PSX-A-11 Late-Breaking: Effects of phytogenic supplementation on growth performance and intestinal barrier function in nursery pigs challenged with E. coli

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 372-374
Author(s):  
Rachita Maniyar ◽  
Bonjin Koo ◽  
Debora Holanda ◽  
Chengbo Yang ◽  
Karmin O ◽  
...  
Keyword(s):  
Ussing Chamber ◽  
Average Daily Gain ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Barrier Function ◽  
Experimental Unit ◽  
Post Inoculation ◽  
Gut Health ◽  
Weaned Piglets ◽  
Health Promoting ◽  
Fecal Score

Abstract The objective of this study was to investigate the health-promoting effects of red osier dogwood (ROD) extract as an alternative to antibiotics in weaned piglets challenged with enterotoxigenic Escherichia coli (ETEC). Twenty-eight weaned piglets (9.15±0.95 kg BW) confirmed to genetically susceptible to ETEC were individually assigned to one of the four dietary treatments in a completely randomized design. Experimental diets were, negative control (NC), corn-wheat soybean meal diet with no additives; positive control (PC), NC plus antibiotics; ROD1, NC plus 0.1% ROD extract; ROD2, NC plus 0.2% ROD extract. Piglets were orally challenged on d 7 with ETEC F4. Feed disappearance, body weight, fecal score, and rectal temperature were recorded. On d 14, piglets were euthanized to collect intestinal tissue samples for histomorphology and Ussing chamber analysis. Data were analyzed using the MIXED procedure of SAS using individual piglet as the experimental unit. There were no differences (P > 0.10) in histomorphology and intestinal permeability. Piglets fed the NC diet tended (P < 0.10) to have higher average daily gain, post-inoculation than those fed ROD1 or ROD2. Fecal score of piglets fed the PC diet tended to (P < 0.10) or was significantly lower (P < 0.05) than for piglets fed ROD1 or ROD2 on 0 and 2 days post-inoculation (dpi), respectively. On 0 dpi, piglets fed the ROD1 diet had significantly higher (P < 0.05) body temperature than those fed PC or ROD2. In conclusion, ROD extract supplementation might have some health-promoting effects on ETEC challenged piglets but could not improve gut health to the same extent as antibiotics.

scholarly journals Detection of Bifidobacterium animalis subsp. lactis (Bb12) in the Intestine after Feeding of Sows and Their Piglets

2008 ◽  
Vol 74 (20) ◽  
pp. 6338-6347 ◽  
Author(s):  
Gloria Solano-Aguilar ◽  
Harry Dawson ◽  
Marta Restrepo ◽  
Kate Andrews ◽  
Bryan Vinyard ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Protein Biosynthesis ◽  
Bifidobacterium Longum ◽  
Elongation Factor ◽  
Single Copy ◽  
Bifidobacterium Animalis ◽  
Gene Copies ◽  
Intestinal Contents ◽  
High Level

ABSTRACT A real-time PCR method has been developed to distinguish Bifidobacterium animalis subspecies in the gastrointestinal tracts of pigs. Identification of a highly conserved single-copy tuf gene encoding the elongation factor Tu involved in bacterial protein biosynthesis was used as a marker to differentiate homologous Bifidobacterium animalis subsp. lactis (strain Bb12) from Bifidobacterium animalis subsp. animalis, as well as Bifidobacterium suis, Bifidobacterium breve, Bifidobacterium longum, several species of Lactobacillus, and Enterococcus faecium. Real-time PCR detection of serially diluted DNA extracted from a pure culture of Bb12 was linear for bacterial numbers ranging from 10 to 10,000 tuf gene copies per PCR (r 2 = 0.99). Relative differences in Bb12 bacterial numbers in pigs fed daily with Bb12 were determined after detection of Bb12 tuf gene copies in DNA extracted from the intestinal contents. Piglets treated with Bb12 immediately after birth maintained a high level of Bb12 in their large intestines with continuous daily administration of Bb12. Piglets born to Bb12-treated sows during the last third of their gestation and also treated with Bb12 at birth (T/T group) had a higher number of Bb12 organisms per gram of intestinal contents compared to placebo-treated piglets born to placebo-treated sows (C/C group), Bb12-treated sows (T/C group), or piglets born to placebo sows but treated with Bb12 immediately after birth (C/T group). In addition, there was a significant increase in gene expression for Toll-like receptor 9 (TLR9) in piglets from the T/T group, with no change in TLR2 and TLR4. These findings suggest that the tuf gene represents a specific and functional marker for detecting Bifidobacterium animalis subsp. lactis strain Bb12 within the microbiota of the intestine.

scholarly journals Husbandry practices and gut health outcomes in weaned piglets: A review

2017 ◽  
Vol 3 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Balachandar Jayaraman ◽  
Charles M. Nyachoti
Keyword(s):  
Health Outcomes ◽  
Gut Health ◽  
Weaned Piglets ◽  
Husbandry Practices

scholarly journals Exopolysaccharides Produced by Probiotic Strains Modify the Adhesion of Probiotics and Enteropathogens to Human Intestinal Mucus

2006 ◽  
Vol 69 (8) ◽  
pp. 2011-2015 ◽  
Author(s):  
PATRICIA RUAS-MADIEDO ◽  
MIGUEL GUEIMONDE ◽  
ABELARDO MARGOLLES ◽  
CLARA G. de los REYES-GAVILÁN ◽  
SEPPO SALMINEN
Keyword(s):  
Salmonella Enterica Serovar Typhimurium ◽  
Bifidobacterium Longum ◽  
Lactobacillus Rhamnosus ◽  
Physiological Role ◽  
Adhesion Properties ◽  
Bifidobacterium Animalis ◽  
Intestinal Mucus ◽  
Serovar Typhimurium ◽  
Probiotic Strains ◽  
Encoding Genes

Exopolysaccharides (EPSs) are exocellular polymers present in the surface of many bacteria, including Lactobacillus and Bifidobacterium. The genome sequence of several strains revealed the presence of EPS-encoding genes. However, the physiological role that EPSs play in the bacterial ecology still remains uncertain. In this study, we have assessed the effect of EPSs produced by Lactobacillus rhamnosus GG, Bifidobacterium longum NB667, and Bifidobacterium animalis IPLA-R1 on the adhesion of probiotic and enteropathogen strains to human intestinal mucus. The EPS fraction GG had no significant effect on the adhesion of L. rhamnosus GG and B. animalis IPLA-R1. However, the EPS fractions NB667 and IPLA-R1 significantly reduced the adherence of both probiotic strains. In contrast, the three EPS fractions increased the adhesion of Enterobacter sakazakii ATCC 29544 and Escherichia coli NCTC 8603. Higher adherence of Salmonella enterica serovar Typhimurium ATCC 29631 and Clostridium difficile ATCC 9689 was detected in the presence of the EPS fractions GG and NB667. In general, these effects were obtained at EPS concentrations of up to 5 mg/ml, and they were EPS dose dependent. The competitive exclusion of probiotics in the presence of EPS could suggest the involvement of these biopolymers in the adhesion to mucus. The increase in the adherence of enteropathogens could be explained if components of the pathogen surface are able to bind to specific EPSs and the bound EPSs are able to adhere to mucus. To the best of our knowledge, this is the first work reporting the effect of EPSs from probiotics on bacterial adhesion properties.

Sign in / Sign up

Export Citation Format

Share Document