scholarly journals Development and Use of a Real-Time Quantitative PCR Method for Detecting and Quantifying Equol-Producing Bacteria in Human Faecal Samples and Slurry Cultures

2017 ◽  
Vol 8 ◽  
Author(s):  
Lucía Vázquez ◽  
Lucía Guadamuro ◽  
Froilán Giganto ◽  
Baltasar Mayo ◽  
Ana B. Flórez
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Quantitative Pcr ◽  
Faecal Samples ◽  
Pcr Method

scholarly journals Development of a Real-Time qPCR Method for Detection and Enumeration of Mycobacterium spp. in Surface Water

2010 ◽  
Vol 76 (21) ◽  
pp. 7348-7351 ◽  
Author(s):  
Nicolas Radomski ◽  
Françoise S. Lucas ◽  
Régis Moilleron ◽  
Emmanuelle Cambau ◽  
Sophie Haenn ◽  
...  
Keyword(s):  
Surface Water ◽  
16S Rrna ◽  
Real Time ◽  
Quantitative Pcr ◽  
Environmental Samples ◽  
Real Time Quantitative Pcr ◽  
Qpcr Method ◽  
Pcr Method ◽  
Real Time Qpcr ◽  
Mycobacterium Spp

ABSTRACT A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).

scholarly journals Real-Time Quantitative PCR for Assessment of Abundance of Pseudoalteromonas Species in Marine Samples

2004 ◽  
Vol 70 (4) ◽  
pp. 2373-2382 ◽  
Author(s):  
Torben L. Skovhus ◽  
Niels B. Ramsing ◽  
Carola Holmström ◽  
Staffan Kjelleberg ◽  
Ingela Dahllöf
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Green Alga ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Rrna Gene ◽  
Marine Animals ◽  
Real Time Quantitative Pcr ◽  
Marine Samples ◽  
Marine Habitats ◽  
Pcr Method

ABSTRACT A real-time quantitative PCR (RTQ-PCR) method for measuring the abundance of Pseudoalteromonas species in marine samples is presented. PCR primers targeting a Pseudoalteromonas-specific region of the 16S rRNA gene were tested at three different levels using database searches (in silico), a selection of pure cultures (in vitro), and a combined denaturing gradient gel electrophoresis and cloning approach on environmental DNA (in situ). The RTQ-PCR method allowed for the detection of SYBR Green fluorescence from double-stranded DNA over a linear range spanning six orders of magnitude. The detection limit was determined as 1.4 fg of target DNA (1,000 gene copies) measured in the presence of 20 ng of nontarget DNA from salmon testes. In this study, we discuss the importance of robust post-PCR analyses to overcome pitfalls in RTQ-PCR when samples from different complex marine habitats are analyzed and compared on a nonroutine basis. Representatives of the genus Pseudoalteromonas were detected in samples from all investigated habitats, suggesting a widespread distribution of this genus across many marine habitats (e.g., seawater, rocks, macroalgae, and marine animals). Three sample types were analyzed by RTQ-PCR to determine the relative abundance of Pseudoalteromonas ribosomal DNA (rDNA) compared to the total abundance of eubacterial rDNA. The rDNA fractions of Pseudoalteromonas compared to all Eubacteria were 1.55% on the green alga Ulva lactuca, 0.10% on the tunicate Ciona intestinalis, and 0.06% on the green alga Ulvaria fusca.

scholarly journals SNPs and real-time quantitative PCR method for constitutional allelic copy number determination, the VPREB1 marker case

2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Marcello Frigerio ◽  
Elena Passeri ◽  
Tiziana de Filippis ◽  
Daniela Rusconi ◽  
Rea Valaperta ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Copy Number ◽  
Real Time Quantitative Pcr ◽  
Copy Number Determination ◽  
Pcr Method ◽  
Allelic Copy Number
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